Exploration of Novel Phytocomponents of Siddha Formulation Pungampoo Chooranam by Qualitative, Quantitative and Fluorescent analysis

Abstract

The importance of medicinal plant in drug development is known to us and humans have used them for different diseases from the beginning of human history. Traditional folk treatment from wild plants has always guided researchers to search for novel medications to develop healthy life for humans and animals. In addition, some medicinal plants are still obscured within the plants which need to be scientifically evaluated. Pongamia pinnata is one such Indian traditional herb which offers numerous therapeutic benefits to mankind since several centuries. Siddha system of traditional medicine uses several valuable medicinal herbs for its formulations. Pungampoo Chooranam (PPC) is a novel siddha preparation which majorly comprises of Pongamia pinnata .The main aim of the present investigation is to carry out the qualitative, quantitative, fluorescent analysis and sterility evaluation of the formulation PPC. The result of the qualitative phytochemical analysis shows presence of alkaloids, steroids, triterpenoids, phenols, tannins, and carbohydrates. Results of quantitative analysis reveals that the total alkaloid level of the sample PPC was found to be 0.48 ± 0.05 mg/gm and the level of total tannins was 0.38 ± 0.02 mg/gm, whereas the level of Total phenols was found to be 0.78 ± 0.04 (GAE mg/gm).Further the sample is of highly sterile and free from E-coli, Salmonella, Staphylococcus aureus, Pseudomonas aeruginosa. In conclusion the results of the present investigation render some evidence based information about the siddha formulation Pungampoo Chooranam with respect to the nature and quantity of phytochemical present in it and also about the sterility and pattern of fluorescent emitting nature of the formulation PPC to the future researchers.