Exploration of surfactin production by newly isolated Bacillus and Lysinibacillus strains from food-related sources

Akintayo, Stephen Olusanmi; Treinen, Chantal; Vahidinasab, Maliheh; Pfannstiel, Jens; Bertsche, Ute; Fadahunsi, I. F.; Oellig, Claudia; Granvogl, Michael; Henkel, Marius; Lilge, Lars; Hausmann, Rudolf

doi:10.1111/lam.13731

Cited by 13 publications

(11 citation statements)

References 31 publications

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“…OF-1, produces an anti-pneumococcal compound of proteinaceous nature that we named lysinicin OF. A few previous studies have reported that members of the genus Lysinibacillus produce antimicrobials of proteinaceous nature such as heat-labile bacteriocins (inactivated >80 °C) and lipopeptides [59–61]. Since lysinicin OF could resist both heat (100 °C) and polymyxin acylase treatment (cleaves off the peptide part of lipopeptides), we concluded that it most probably is a peptide.…”

Section: Discussionmentioning

confidence: 79%

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

et al. 2023

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Infections caused by antibiotic-resistant Streptococcus pneumoniae are of growing concern for healthcare systems, which need new treatment options. Screening microorganisms in terrestrial environments has proved successful for discovering antibiotics, while production of antimicrobials by marine microorganisms remains underexplored. Here we have screened microorganisms sampled from the Oslo Fjord in Norway for production of molecules that prevent the human pathogen S. pneumoniae from growing. A bacterium belonging to the genus Lysinibacillus was identified. We show that this bacterium produces a molecule that kills a wide range of streptococcal species. Genome mining in BAGEL4 and AntiSmash suggested that it was a new antimicrobial compound, and we therefore named it lysinicin OF. The compound was resistant to heat (100 °C) and polymyxin acylase but susceptible to proteinase K, showing that it is of proteinaceous nature, but most probably not a lipopeptide. S. pneumoniae became resistant to lysinicin OF by obtaining suppressor mutations in the ami locus, which encodes the AmiACDEF oligo peptide transporter. We created ΔamiC and ΔamiEF mutants to show that pneumococci expressing a compromised Ami system were resistant to lysinicin OF. Furthermore, by creating mutants expressing an intact but inactive Ami system (AmiED184A and AmiFD175A) we could conclude that the lysinicin OF activity depended on the active form (ATP-hydrolysing) of the Ami system. Microscopic imaging and fluorescent labelling of DNA showed that S. pneumoniae treated with lysinicin OF had an average reduced cell size with condensed DNA nucleoid, while the integrity of the cell membrane remained intact. The characteristics and possible mode of action of lysinicin OF are discussed.

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Section: Discussionmentioning

confidence: 79%

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

et al. 2023

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“…To check the antiadherent activity, the P. aeruginosa culture was incubated for 72 h with different concentrations (2. 5,5,10,20,30,40,50,100,150, and 250 μg/mL) of SUR-AgNPs in 3 mL glass tubes. After treatment, the tubes were washed with PBS (pH 7.4), and the biofilm was stained using crystal violet dye (Figure 3A).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Its amphiphilic properties allows surfactin to be used in both hydrophobic and hydrophilic environments and on phospholipid membranes. 20 Based on its interactive properties with plasma membrane and other biomolecules, 21 it was hypothesized that conjugation of surfactin to NPs could synergistically enhance their antimicrobial activity and facilitate the NPs delivery through the complex environment (e.g., cysts and biofilms etc.) developed by bacteria to escape antimicrobial agents.…”

Section: ■ Introductionmentioning

confidence: 99%

Surfactin-Conjugated Silver Nanoparticles as an Antibacterial and Antibiofilm Agent against Pseudomonas aeruginosa

Rahman,

Sarwar,

Khaliq

et al. 2023

ACS Appl. Mater. Interfaces

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The emergence of antimicrobial resistance is an alarming global health concern and has stimulated the development of novel functional nanomaterials to combat multi-drug-resistant (MDR) bacteria. In this work, we demonstrate for the first time the synthesis and application of surfactin-coated silver nanoparticles as an efficient antibacterial and antibiofilm agent against the drug-resistant bacteria Pseudomonas aeruginosa for safe dermal applications. Our in vivo studies showed no significant superficial dermal irritation, edema, and erythema, while microscopic analysis revealed that surfactin-coated silver nanoparticles caused no pathological alterations at the applied concentrations. These results support the potential use of surfactin-coated silver nanoparticles against drug-resistant bacterial biofilm infections and in skin wound dressing applications.

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“…A volume of 1 ml of dilutions of 10 −12 , 10 −10 and 10 −8 was plated on nutrient agar (NA). The plates were incubated at 37 °C for 48 h. Distinct colonies were selected based on colony morphology on plate and were subcultured three times on fresh plates to obtain clear single colonies from one bacterial species [5].…”

Section: Isolation and Ecologymentioning

confidence: 99%

“…They have also been isolated from water [4] and the rhizosphere of plants [2]. In a recent study, several strains were also isolated from food and food production sites [5]. Ecological functions of strains of this genus include nitrogen fixation, phosphorus solubilization, adsorption of toxic metals [6], as well as antimicrobial activity against bacteria and fungi [7].…”

Section: Introductionmentioning

confidence: 99%

Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans

Akintayo,

Neumann,

Fischer

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01T was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening process from food-related sources. IRB4-01T grew at 10–50 °C (optimum, 35–37 °C), pH 6–10 (optimum, pH 7) and in 0–6 % NaCl (optimum, 1–3 %). Phylogenetic analyses based on 16S rRNA and combined short- and long-read genome sequencing revealed that IRB4-01T is closely related to Lysinibacillus cavernae SYSU K30005T and Lysinibacillus boronitolerans 10aT. The cell-wall peptidoglycan type was A4α (Lys–Asp), containing the diagnostic diamino acid lysine. The major polar lipids in strain IRB4-01T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid, while the predominant menaquinone was MK-7. The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. Genomic DNA G+C content was 37.4 mol%, while the digital DNA–DNA hybridization revealed 33.6 and 32.3 % relatedness to L. cavernae SYSU K30005T and L. boronitolerans 10aT, respectively. Based on phenotypic, physiological and chemotaxonomic characteristics, as well as genome comparisons, strain IRB4-01T represents a novel species of the genus Lysinibacillus , for which the name Lysinibacillus irui sp. nov. is proposed. The type strain is IRB4-01T (NCIMB 15452T=LMG 32887T). Hybrid genome data are provided on the NCBI database using the Bioproject number PRJNA906010 and accession numbers CP113527 and CP113528. Additionally, a representative 16S rRNA sequence is available with the GenBank accession number OQ566940.

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Exploration of surfactin production by newly isolated Bacillus and Lysinibacillus strains from food-related sources

Cited by 13 publications

References 31 publications

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

A novel proteinaceous molecule produced by Lysinibacillus sp. OF-1 depends on the Ami oligopeptide transporter to kill Streptococcus pneumoniae

Surfactin-Conjugated Silver Nanoparticles as an Antibacterial and Antibiofilm Agent against Pseudomonas aeruginosa

Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans

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