Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

Lègba, Boris; Dougnon, Victorien; Ahoyo, Angèle; Agbankpé, Alidehou Jerrold; Hounmanou, Yaovi Mahuton Gildas; Aniambossou, Alidah; Hounsa, Edna; Fabiyi, Kafayath; Amadou, Affousssath; Assogba, Phénix; Bankolè, Honoré; Dougnon, Jacques; Baba-Moussa, Lamine

doi:10.4081/mm.2017.6998

Cited by 12 publications

(17 citation statements)

References 13 publications

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“…Toxicological, chemical and antibacterial (to ten enteropathogens) characterization have been done. This study showed interesting contents in polyphenols and flavonoids and an effective antibacterial activity at 100 mg/mL with Minimal Inhibitory Concentration (MIC) between 100 and 25 mg/mL and inhibition diameters between 7.5 and 21 mm [16].…”

Section: Introductionmentioning

confidence: 80%

“…Method described by Legba et al [16] were used. After their collection, the organs (leaves and root) were dried at a temperature of 16°C in the laboratory.…”

Section: Production Of Aqueous and Ethanolic Extractsmentioning

confidence: 99%

“…Inoculum was obtained by emulsifying a 24-h pure colony of each strain from the Mueller Hinton (MH) medium in physiological water (5 ml). A turbidity of 0.5 Mc Farland is obtained [16].…”

Section: Production Of Aqueous and Ethanolic Extractsmentioning

confidence: 99%

“…After 1 h pre-diffusion at room temperature, the petri dish was incubated at 37°C. After 24 h of incubation, the dish was examined and the zones of inhibition were measured [16]. Method used by Legba et al [33] were used for determination of Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC).…”

Section: Production Of Aqueous and Ethanolic Extractsmentioning

confidence: 99%

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Evaluation of in-vivo anti-Salmonella activity of Uvaria chamae, Lantana camara and Phyllantus amarus used in Benin, West Africa

Lègba

Dougnon

Chabi³

et al. 2020

BMC Vet Res

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Background: Uvaria chamae (Annonaceae), Phyllantus amarus (Phyllantaceae) and Lantana camara (Verbenaceae) are empirically alleged to be used as Beninese medicinal plants in the treatment of salmonellosis. This study aimed to produce scientific data on in vitro and in vivo efficacy of Uvaria chamae, Lantana camara and Phyllantus amarus on multiresistant Salmonella spp isolated in Benin. Results: After performing in vitro tests on aqueous and ethanolic extracts of these plants, only the aqueous extract of Uvaria chamae (leaves) showed the best anti-Salmonella's activity and was used for this in vivo experiment. The induction of salmonellosis revealed 9 × 10 8 CFU/ml was the optimal concentration triggering and maintaining symptoms in chicks. This infective concentration was used for in vivo assessment. Twenty-four hours post inoculation, the symptoms of salmonellosis (wet cloaca, diarrhea stools and somnolence) were observed in infected groups. After 7 days of treatment, the reduction of bacterial load at 100 mg/L, 200 mg/L, 400 mg/L of the extract was respectively 85%, 52.38% and 98% for Uvaria chamae, Phyllantus amarus and Lantana camara in the chick's groups infected with Salmonella Typhimurium ATCC 14028. On the other hand, colistin completely cancelled the bacterial load (reduction rate of 100%). With the groups infected with Salmonella spp (virulent strain), the reduction rate of bacterial load at 100 mg/L, 200 mg/L, 400 mg/L of extract was 0%, 98.66%, and 99.33%. The extracts at 200 and 400 mg/L were more active than colistin, which reduced the bacterial load by 33.33%. The toxicity tests did not show any negative effect of Colistin and the Uvaria chamae's extract on the biochemical and hematological parameters of the chicks. Conclusion:The aqueous extract of Uvaria chamae is active in vitro and in vivo on multiresistant strains of Salmonella enterica. This plant is a good candidate for the development of an improved traditional medicine for the management of salmonellosis.

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Section: Introductionmentioning

confidence: 80%

“…Method described by Legba et al [16] were used. After their collection, the organs (leaves and root) were dried at a temperature of 16°C in the laboratory.…”

Section: Production Of Aqueous and Ethanolic Extractsmentioning

confidence: 99%

“…Inoculum was obtained by emulsifying a 24-h pure colony of each strain from the Mueller Hinton (MH) medium in physiological water (5 ml). A turbidity of 0.5 Mc Farland is obtained [16].…”

Section: Production Of Aqueous and Ethanolic Extractsmentioning

confidence: 99%

Section: Production Of Aqueous and Ethanolic Extractsmentioning

confidence: 99%

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Evaluation of in-vivo anti-Salmonella activity of Uvaria chamae, Lantana camara and Phyllantus amarus used in Benin, West Africa

Lègba

Dougnon

Chabi³

et al. 2020

BMC Vet Res

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“…In Benin, an ethnopharmacological survey performed in Benin identified 57 medicinal plants used by herbalists in the traditional management of Senna siamea (Lam) was one of the most quoted plants [8]. A study performed by Legba et al [9] provided interesting data on the antibacterial activity of Senna siamea on enteric pathogens, and its chemical and toxicological characteristics. In addition, several authors demonstrated in vitro anti-Salmonella activity of S. siamea.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Anti-Salmonella Activity of Aqueous and Ethanolic Extract of Senna siamae, Used in Traditional Management of Salmonellosis in Benin

Lègba¹,

Dougnon²,

Agbankpé³

et al. 2020

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Recent ethnopharmacological data cited Senna siamea as one of the most widely used medicinal plants in the management of salmonellosis in Benin. However, data related to its activity on non-typhoidal Salmonella spp are limited. This study aimed to assess the antibacterial activity of Senna siamea on multidrug-resistant Salmonella. Ethanolic and aqueous extracts of S. siamea were tested for their antibacterial activity on four multidrug-resistant Salmonella: Salmonella Typhimurium ATCC 14028 and three Salmonella spp. isolated from animals intended for human consumption in Benin. Well diffusion technique combined with the determination by microdilution of Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) were used for antibacterial testing. From antibacterial testing, inhibition diameters of the extracts ranged from 7 to 11 mm, for the susceptible strains. Colistin (reference antibiotic) was active on all Salmonella spp. with inhibition diameters between 18 and 19 mm. The MICs ranged from 3.125 to 25 mg/ml while MBCs of the extracts are greater than 100 mg/ml, so none of the extracts have antibacterial power (p.a). From these results, it appears that the use of Senna siamea in the traditional treatment of salmonellosis is justified. These results must be valued in the development of anti-salmonella phytomedicines.

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Toxicological Characterization of Six Plants of the Beninese Pharmacopoeia Used in the Treatment of Salmonellosis

Lègba

Dougnon

Déguénon

et al. 2019

Journal of Toxicology

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Recent studies reported interesting ethnopharmacological, antibacterial, and phytochemical data on some medicinal plants used in the traditional treatment of salmonellosis in Benin. Unfortunately, very little data exists on the toxicity of these species. This study aims to evaluate chemical characteristic of six Benin pharmacopoeial plants used in the traditional treatment of salmonellosis in Benin. The acute toxicity of aqueous and ethanolic extracts ofPsidium guajava,Vernonia amygdalina,Cajanus cajan,Phyllanthus amarus,Uvaria chamae, andLantana camarawas evaluated according to OECD Guideline 423 at a single dose of 2000 mg/kg body weight on Wistar rats. Histological sections were performed on the liver and kidneys to confirm hematological and biochemical data. The content of aluminum, chromium, cadmium, copper, iron, lead, zinc, arsenic, selenium, and manganese was measured in 10 mg of each extract by the inductively coupled plasma optical emission spectroscopy (ICPOES) method. The results of our study generally show the absence of significant effect of the extracts on the hematological and biochemical parameters of the rats. However, with the exception of the aqueous and ethanolic extracts ofPsidium guajavaroot and the ethanolic extract ofPhyllanthus amarus(P>0.05), all the extracts have a significant effect on the aspartate aminotransferase (ASAT) level, with a variable threshold of significance (0.0001< P ≤ 0.05). No mortalities and no renal histological conditions were recorded in the treated rats. In general, the heavy metal contents of the extracts do not exceed the standards set by the WHO/FDA except for a few extracts. Arsenic was not detected in any extract, while aluminum and chromium were detected at levels above the WHO/FDA standards. On the basis of these data, it appears that the six plants studied do not show any toxicity. In view of the pharmacological and chemical data previously available, these plants are good candidates for the development of improved traditional medicines with antibacterial and particularly anti-Salmonellaproperties.

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Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

Cited by 12 publications

References 13 publications

Evaluation of in-vivo anti-Salmonella activity of Uvaria chamae, Lantana camara and Phyllantus amarus used in Benin, West Africa

Evaluation of in-vivo anti-Salmonella activity of Uvaria chamae, Lantana camara and Phyllantus amarus used in Benin, West Africa

Assessment of Anti-Salmonella Activity of Aqueous and Ethanolic Extract of Senna siamae, Used in Traditional Management of Salmonellosis in Benin

Toxicological Characterization of Six Plants of the Beninese Pharmacopoeia Used in the Treatment of Salmonellosis

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