Exploration of the cellular mediated immunity by the blastogenesis test during chronic brucellosis in human

Person, J.M; Frottier, J; Garrec, Y. Le; Barrat, F.; Bastin, R; Pilet, C

doi:10.1016/0147-9571(87)90035-x

Comparative Immunology, Microbiology and Infectious Diseases

1987

DOI: 10.1016/0147-9571(87)90035-x

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Exploration of the cellular mediated immunity by the blastogenesis test during chronic brucellosis in human

J.M Person

J Frottier

Y. Le Garrec

et al.

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1988

1993

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Cited by 3 publications

(3 citation statements)

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“…Immunity to intracellular bacteria is mainly dependent on activation of the appropriate host cellular response (8,13). Several lines of evidence suggest that cell-mediated immunity can limit the pathogenesis of brucellosis (4,23,34). B. abortus proteins that induce a T-lymphocyte-mediated response are most likely responsible for the observed protection.…”

mentioning

confidence: 99%

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes

1993

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BruceUa abortus proteins from virulent S2308 expressed from a pBluescript II SK-genomic library stimulated peripheral blood mononuclear (PBM) cell proliferation from cattle vaccinated with B. abortus S19. The method described here permits a rapid and directed approach to isolate genes encoding antigens of B. abortus that interact with lymphocytes primed to the living bacterium. The supernatants from the bacterial host JM109 (DE3) were cultured with freshly isolated bovine PBM cells. A total of 300 clones were evaluated. Ten clones were identified that stimulated T-lymphocyte proliferation. Among them, one clone with a 2.5-kb insert stimulated T-lymphocyte proliferation in all three animals, suggesting that the proteins encoded by genes within this fragment may represent immunodominant antigens. DNA sequencing of this clone reveals two large open reading frames (ORFs). ORF II has a high degree of similarity to the Escherichia coli ssb gene, which codes for the single-stranded DNA binding protein. ORF I, in the opposite direction to ORF II, shows similarity to the N terminus of the E. coli uvrA gene, which codes for one of the three subunits of the E. coli ABC excision nuclease. The observation that the PBM cells recognized and proliferated in response to proteins expressed from single clones provides a novel strategy to select bacterial antigens that may prove useful in designing alternative vaccines against brucellosis.B. abortus genomic library. The library of B. abortus S2308, a virulent strain of B. abortus, was constructed in the HindIII restriction site of the pBluescript II SK-phagemid. 5339 on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

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confidence: 99%

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes

1993

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“…Stimulation of cellular responses are important in the development of immunity to facultative intracellular pathogens (6,13), such as Brucella organisms (18). Protective immunity induced against one species of Brucella has been demonstrated in many animal species (7,9,20), including cows (19), to be effective for protection against other Brucella species.…”

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confidence: 99%

Sodium dodecyl sulfate- and salt-extracted antigens from various Brucella species induce proliferation of bovine lymphocytes

Brooks-Worrell

Splitter

1992

Infect Immun

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Oneand two-dimensional gel electrophoresis and cellular immunoblotting were used to compare the protein profiles and immunogenic capabilities of salt-and sodium dodecyl sulfate-extracted components isolated from different BruceUla species. Cellular immunoblotting demonstrated that freshly isolated bovine peripheral blood mononuclear cells proliferated to similar molecular mass components from the various Brucella extractions. One-and two-dimensional gel electrophoresis showed similarities in protein profiles among the different BruceUla species that correlated with lymphocyte reactivity. The results presented in this study provide preliminary evidence that common proteins that induce lymphocyte proliferation are present among different Brucella species, suggesting that a genuswide subunit vaccine may be feasible.

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“…Bruicella abortus, a facultative intracellular bacterium, causes febrile disease in humans (1) and contagious abortions in many animal species (38). De-velopment of a protective response to B. abortus requires activation of host cell-mediated responses (2,22,28). Efforts to understand and identify the bacterial antigens associated with activation of host cellular responses has led researchers to isolate and characterize outer membrane proteins from many bacteria (25,26), including B. abortius (8,29,30).…”

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confidence: 99%

Determination of bovine lymphocyte responses to extracted proteins of Brucella abortus by using protein immunoblotting

Brooks-Alder

Splitter

1988

Infect Immun

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Isolation and identification of Brucella antigenic determinants important to cellular responses have been difficult. In this study, bovine peripheral blood mononuclear (PBM) cells from cattle vaccinated with Brucella abortus 19 proliferated to extracted bacterial proteins blotted onto nitrocellulose. Proteins were extracted from gamma-irradiated B. abortus 19 with a sodium dodecyl sulfate extraction buffer. The extracted proteins were * Corresponding author.

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Exploration of the cellular mediated immunity by the blastogenesis test during chronic brucellosis in human

Cited by 3 publications

References 2 publications

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes

Isolation of Brucella abortus ssb and uvrA genes from a genomic library by use of lymphocytes as probes

Sodium dodecyl sulfate- and salt-extracted antigens from various Brucella species induce proliferation of bovine lymphocytes

Determination of bovine lymphocyte responses to extracted proteins of Brucella abortus by using protein immunoblotting

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