Explorations and Applications of Enzyme-linked Bioremediation of Synthetic Dyes

Ogola, Henry Joseph Oduor; Ashida, Hiroyuki; Ishikawa, Takahiro; Sawa, Yoshihiro

doi:10.5772/60753

Cited by 10 publications

(7 citation statements)

References 157 publications

(235 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzymes responsible for dye decolorization belong to the family of oxidoreductases (EC: 1), which catalyze oxidation and reduction reactions, finding application in various domains varying from diagnostics to wastewater treatment and production of chemicals or potentially biofuels. − They have been studied extensively for dye decolorzation and bioremediation, with much research focusing on the oxidative action of laccases and peroxidases as well as the reductive action of azoreductases (azo dye specific enzymes), with many review papers available targeting dye degradation in general ,,− or focusing on azo dyes, ,, but none focusing specifically on anthraquinone dyes.…”

Section: Focus On Enzymatic Decolorizationmentioning

confidence: 99%

Degradation of Anthraquinone Dyes from Effluents: A Review Focusing on Enzymatic Dye Degradation with Industrial Potential

Routoula

Patwardhan

2020

Environ. Sci. Technol.

437

View full text Add to dashboard Cite

Up to 84 000 tons of dye can be lost in water, and 90 million tons of water are attributed annually to dye production and their application, mainly in the textile and leather industry, making the dyestuff industry responsible for up to 20% of the industrial water pollution. The majority of dyes industrially used today are aromatic compounds with complex, reinforced structures, with anthraquinone dyes being the second largest produced in terms of volume. Despite the progress on decolorization and degradation of azo dyes, very little attention has been given to anthraquinone dyes. Anthraquinone dyes pose a serious environmental problem as their reinforced structure makes them difficult to degrade naturally. Existing methods of decolorization might be effective but are neither efficient nor practical due to extended time, space, and cost requirements. Attention should be given to the emerging routes for dye decolorization via the enzymatic action of oxidoreductases, which have already a strong presence in various other bioremediation applications. This review will discusses the presence of anthraquinone dyes in the effluents and ways for their remediation from dyehouse effluents, focusing on enzymatic processes.

show abstract

Section: Focus On Enzymatic Decolorizationmentioning

confidence: 99%

Degradation of Anthraquinone Dyes from Effluents: A Review Focusing on Enzymatic Dye Degradation with Industrial Potential

Routoula

Patwardhan

2020

Environ. Sci. Technol.

437

View full text Add to dashboard Cite

show abstract

“…Bacteria have enzymes as such oxidoreductive, veratryl alcohol oxidase, azoreductase, laccase, and peroxidase, which allow them to break down the dyes in industrial wastewater and effluents [ 41 , 42 ]. Bacterial degradation of azo dyes in textile and tanning effluents usually involves two steps.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Azo Dye Methyl Red by Pseudomonas aeruginosa: Optimization of Process Conditions

Ikram

Naeem

Zahoor

et al. 2022

IJERPH

View full text Add to dashboard Cite

Water pollution due to textile dyes is a serious threat to every life form. Bacteria can degrade and detoxify toxic dyes present in textile effluents and wastewater. The present study aimed to evaluate the degradation potential of eleven bacterial strains for azo dye methyl red. The optimum degradation efficiency was obtained using P. aeruginosa. It was found from initial screening results that P. aeruginosa is the most potent strain with 81.49% degradation activity and hence it was subsequently used in other degradation experiments. To optimize the degradation conditions, a number of experiments were conducted where only one variable was varied at a time and where maximum degradation was observed at 20 ppm dye concentration, 1666.67 mg/L glucose concentration, 666.66 mg/L sodium chloride concentration, pH 9, temperature 40 °C, 1000 mg/L urea concentration, 3 days incubation period, and 66.66 mg/L hydroquinone (redox mediator). The interactive effect of pH, incubation time, temperature, and dye concentration in a second-order quadratic optimization of process conditions was found to further enhance the biodegradation efficiency of P. aeruginosa by 88.37%. The metabolites of the aliquot mixture of the optimized conditions were analyzed using Fourier transform infrared (FTIR), GC-MS, proton, and carbon 13 Nuclear Magnetic Resonance (NMR) spectroscopic techniques. FTIR results confirmed the reduction of the azo bond of methyl red. The Gas Chromatography–Mass Spectrometry (GC-MS) results revealed that the degraded dye contains benzoic acid and o-xylene as the predominant constituents. Even benzoic acid was isolated from the silica gel column and identified by 1H and 13C NMR spectroscopy. These results indicated that P. aeruginosa can be utilized as an efficient strain for the detoxification and remediation of industrial wastewater containing methyl red and other azo dyes.

show abstract

“…Moreover, mixed bacterial cultures are more efficient than pure cultures in decolorization with a higher rate and complete degradation with multiple enzymes that attack the dye molecule at different sites [ 16 ]. Biodegradation of wastewater occurs by dye-degrading oxidoreductive enzymes, such as tyrosinases, laccases, peroxidases, veratryl alcohol oxidase, and azoreductases [ 17 , 18 ]. Azoreductases are one of the most common enzymes found in many bacteria, such as Pseudomonas sp., Enterococcus faecalis YZ 66, and Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation, Decolorization, and Detoxification of Di-Azo Dye Direct Red 81 by Halotolerant, Alkali-Thermo-Tolerant Bacterial Mixed Cultures

Abdeltawab

Farag

2022

Microorganisms

View full text Add to dashboard Cite

Azo dyes impact the environment and deserve attention due to their widespread use in textile and tanning industries and challenging degradation. The high temperature, pH, and salinity used in these industries render industrial effluent decolorization and detoxification a challenging process. An enrichment technique was employed to screen for cost-effective biodegraders of Direct Red 81 (DR81) as a model for diazo dye recalcitrant to degradation. Our results showed that three mixed bacterial cultures achieved ≥80% decolorization within 8 h of 40 mg/L dye in a minimal salt medium with 0.1% yeast extract (MSM-Y) and real wastewater. Moreover, these mixed cultures showed ≥70% decolorization within 24 h when challenged with dye up to 600 mg/L in real wastewater and tolerated temperatures up to 60 °C, pH 10, and 5% salinity in MSM-Y. Azoreductase was the main contributor to DR81 decolorization based on crude oxidative and reductive enzymatic activity of cell-free supernatants and was stable at a wide range of pH and temperatures. Molecular identification of azoreductase genes suggested multiple AzoR genes per mixed culture with a possible novel azoreductase gene. Metabolite analysis using hyphenated techniques suggested two reductive pathways for DR81 biodegradation involving symmetric and asymmetric azo-bond cleavage. The DR81 metabolites were non-toxic to Artemia salina nauplii and Lepidium sativum seeds. This study provided evidence for DR81 degradation using robust stress-tolerant mixed cultures with potential use in azo dye wastewater treatment.

show abstract

Explorations and Applications of Enzyme-linked Bioremediation of Synthetic Dyes

Cited by 10 publications

References 157 publications

Degradation of Anthraquinone Dyes from Effluents: A Review Focusing on Enzymatic Dye Degradation with Industrial Potential

Degradation of Anthraquinone Dyes from Effluents: A Review Focusing on Enzymatic Dye Degradation with Industrial Potential

Biodegradation of Azo Dye Methyl Red by Pseudomonas aeruginosa: Optimization of Process Conditions

Biodegradation, Decolorization, and Detoxification of Di-Azo Dye Direct Red 81 by Halotolerant, Alkali-Thermo-Tolerant Bacterial Mixed Cultures

Contact Info

Product

Resources

About