Exploring graphene oxide intrinsic electroactivity to elucidate the non-covalent interactions with DNA oligonucleotides

Liang, Yaquan; Ang, Wei Li; Lim, Rachel Rui Xia; Bonanni, Alessandra

doi:10.1039/d1cc06657a

Cited by 5 publications

(2 citation statements)

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“…Many studies have indicated that GO exhibits weak adsorption on DNA. That is, the adsorption capacity of GO for double‐stranded DNA is weaker than that of single‐stranded DNA, and is affected by factors such as temperature, pH, salt concentration, type of organic solvent and temperature 20–22 . Therefore, in the cell lysates of microbial or plant tissues, GO can strongly adsorb a large number of heavy metal ions, proteins and other impurities that affect PCRs, but only a small amount of DNA, resulting in relatively pure DNA in the supernatant after GO sedimentation 23 …”

Section: Introductionmentioning

confidence: 99%

“…That is, the adsorption capacity of GO for double-stranded DNA is weaker than that of single-stranded DNA, and is affected by factors such as temperature, pH, salt concentration, type of organic solvent and temperature. [20][21][22] Therefore, in the cell lysates of microbial or plant tissues, GO can strongly adsorb a large number of heavy metal ions, proteins and other impurities that affect PCRs, but only a small amount of DNA, resulting in relatively pure DNA in the supernatant after GO sedimentation. 23 Therefore, we exploited the advantages of GO to develop a GObased DNA purification method and present a simple procedure for extracting DNA from plant and microbial samples.…”

Section: Introductionmentioning

confidence: 99%

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A simple graphene oxide‐based DNA purification strategy for plant pathogen detection

Zhang,

Wang,

et al. 2024

Pest Management Science

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BACKGROUNDThe on‐site molecular detection of plant pathogens is particularly important for the development of sustainable agriculture. Extracting DNA from plant tissues, microbes, or coexisting environments is complex, labor‐intensive, and time‐consuming. To facilitate this process, we propose a DNA purification strategy based on graphene oxide (GO).RESULTSThe excellent adsorption ability of GO was verified by visualizing changes in its microscopic surface and macroscopic mixture. To further optimize the DNA purification, we determined the optimal GO concentration and treatment time at 95 °C (2 mg/mL and 2 min, respectively). We confirmed that our strategy is effective on plant tissues and various microorganisms, and that the obtained DNA can be directly used for polymerase chain reaction amplification. Combining the proposed GO‐based DNA purification method with the loop‐mediated isothermal amplification method is superior to the cetyltrimethylammonium bromide method and a commercial kit for detecting plant pathogens in terms of the required steps, time, cost, and detection effect.CONCLUSIONWe present a feasible, rapid, simple, and low‐cost DNA purification method with high practical value for scientific applications in plant pathogen detection. This strategy can also provide important technical support for future research on plant–microbial microenvironments.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%