Exploring interactions between xenobiotics, microbiota, and neurotoxicity in zebrafish

Bertotto, Luísa Becker; Catron, Tara R.; Tal, Tamara

doi:10.1016/j.neuro.2019.11.008

Cited by 48 publications

(21 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a larval zebrafish model, a powerful system for assessing the function of host-microbe interactions [10,11], heat-killed bacteria and selected microbe-associated molecular patterns were both insufficient to modify axenic-related hyperactivity [4]. Furthermore, rather than restore normal behavior, monoassociation with either Aeromonas veronii or Vibrio cholerae resulted in a hypoactive phenotype [4].…”

Section: Introductionmentioning

confidence: 99%

Monoassociation with bacterial isolates reveals the role of colonization, community complexity and abundance on locomotor behavior in larval zebrafish

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Across taxa, animals with depleted intestinal microbiomes show disrupted behavioral phenotypes. Axenic (i.e., microbe-free) mice, zebrafish, and fruit flies exhibit increased locomotor behavior, or hyperactivity. The mechanism through which bacteria interact with host cells to trigger normal neurobehavioral development in larval zebrafish is not well understood. Here, we monoassociated zebrafish with either one of six different zebrafish-associated bacteria, mixtures of these host-associates, or with an environmental bacterial isolate. Results As predicted, the axenic cohort was hyperactive. Monoassociation with three different host-associated bacterial species, as well as with the mixtures, resulted in control-like locomotor behavior. Monoassociation with one host-associate and the environmental isolate resulted in the hyperactive phenotype characteristic of axenic larvae, while monoassociation with two other host-associated bacteria partially blocked this phenotype. Furthermore, we found an inverse relationship between the total concentration of bacteria per larvae and locomotor behavior. Lastly, in the axenic and associated cohorts, but not in the larvae with complex communities, we detected unexpected bacteria, some of which may be present as facultative predators. Conclusions These data support a growing body of evidence that individual species of bacteria can have different effects on host behavior, potentially related to their success at intestinal colonization. Specific to the zebrafish model, our results suggest that differences in the composition of microbes in fish facilities could affect the results of behavioral assays within pharmacological and toxicological studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Monoassociation with bacterial isolates reveals the role of colonization, community complexity and abundance on locomotor behavior in larval zebrafish

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…As far as we know, there are no specific works on the microbiome and ABC proteins interaction. However, xenobiotics, like antibiotics, other drugs, and microplastics, can affect the microbiome composition and metabolism and, therefore, the intestinal function in general and the function of ABC proteins in particular (see Bertotto et al, 2019 ; Catron et al, 2019 and citations reviewed therein; Ding et al, 2021 ).…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

Expression and Function of ABC Proteins in Fish Intestine

et al. 2021

View full text Add to dashboard Cite

In fish, the intestine is fundamental for digestion, nutrient absorption, and other functions like osmoregulation, acid-base balance, and excretion of some metabolic products. These functions require a large exchange surface area, which, in turn, favors the absorption of natural and anthropogenic foreign substances (xenobiotics) either dissolved in water or contained in the food. According to their chemical nature, nutrients, ions, and water may cross the intestine epithelium cells’ apical and basolateral membranes by passive diffusion or through a wide array of transport proteins and also through endocytosis and exocytosis. In the same way, xenobiotics can cross this barrier by passive diffusion or taking advantage of proteins that transport physiological substrates. The entry of toxic substances is counterbalanced by an active efflux transport mediated by diverse membrane proteins, including the ATP binding cassette (ABC) proteins. Recent advances in structure, molecular properties, and functional studies have shed light on the importance of these proteins in cellular and organismal homeostasis. There is abundant literature on mammalian ABC proteins, while the studies on ABC functions in fish have mainly focused on the liver and, to a minor degree, on the kidney and other organs. Despite their critical importance in normal physiology and as a barrier to prevent xenobiotics incorporation, fish intestine’s ABC transporters have received much less attention. All the ABC subfamilies are present in the fish intestine, although their functionality is still scarcely studied. For example, there are few studies of ABC-mediated transport made with polarized intestinal preparations. Thus, only a few works discriminate apical from basolateral transport activity. We briefly describe the main functions of each ABC subfamily reported for mammals and other fish organs to help understand their roles in the fish intestine. Our study considers immunohistochemical, histological, biochemical, molecular, physiological, and toxicological aspects of fish intestinal ABC proteins. We focus on the most extensively studied fish ABC proteins (subfamilies ABCB, ABCC, and ABCG), considering their apical or basolateral location and distribution along the intestine. We also discuss the implication of fish intestinal ABC proteins in the transport of physiological substrates and aquatic pollutants, such as pesticides, cyanotoxins, metals, hydrocarbons, and pharmaceutical products.

show abstract

“…Mikroçip çalışmalarından MOA bilgisi sonucu çıkarmak için çok sayıda metot vardır. Zebra balığı beyninde nörofarmasötiklerin etkilerinin araştırıldığı son çalışmalara göre, bu balık türünde görülen etkiler memelilerdekine benzer bir örüntü göstermektedir [68,69]. Bundandır ki türler arası ekstrapolasyonlarla, balıklardan yararlanıp, bu ilaçların uzun dönemli etkileri hakkında tahminler yapabilmek mümkün olmuştur.…”

Section: Etki Mekanizmasını Anlamada Genomik Yaklaşımunclassified

Ecotoxicogenomics Concept and Its Contributions to Ecological Risk Assessment

Şahin

2020

Türk Doğa Ve Fen Dergisi

View full text Add to dashboard Cite

Öz: Ekotoksikolojide, ekotoksikogenomik yaklaşımlar önemli bir alan olmaya başlamıştır. Toksikogenomikler önceleri kimyasalların insanlara risklerini belirlemek amacıyla kullanımdayken, bu konudaki son gelişmeler bu yaklaşımın diğer organizmalara da uygulanabilirliğini göstermiştir. Ekotoksikogenomik, ekosistemi temsil eden ve bireyler üzerinde olduğu kadar ekosistem üzerinde de kimyasalların zararlı etkilerinin çalışıldığı, organizmalara yönelik toksikogenomik bir uygulamadır. Ekotoksikogenomik belli bir toksikanta biyolojik sistemlerin bir yanıtı olarak, öncül gen ekspresyon çalışmalarından gelişmiştir. Zaman içinde olgunlaşan çalışmalar çeşitli-omik alanlarının toksikoloji ve patolojide kullanılmasına olanak sağlamıştır. Bu bağlamda ekolojik risk değerlendirme çalışmalarında çeşitli enzimler ve proteinlerin (örneğin Glutatyon S-transferaz, metallotiyonin, kolinesterazlar, ısı-şok proteinleri) biyobelirteç olarak kullanılması canlılar üzerindeki potansiyel etkilerin gözlemlenmesine olanak sağlamıştır. Ayrıca birçok omurgalı ve omurgasız canlıda toksik etkiye maruz kalmanın belirlenmesinde mikroçip veya gen çiplerinden yararlanılarak hangi genlerin hücrede, dokuda, organda "up/down regüle" olarak ifade edilmesinin belirlenmesi de bu yaklaşımın bir diğer alanıdır. Etki ve genin sonuç özgü örüntüsü, protein ve metabolit profilleri, toksisitenin biyobelirteçleri olarak moleküler değişimleri tanımlamada kullanılmakta ve bu profiller, toksisite mekanizmalarını açıklamayı da sağlamaktadır. Bu yaklaşım ile çok sayıda farklı etkenin etki mekanizmalarını belirlenerek, belli tür ve populasyon alt gruplarında bu mekanizmaları yorumlayabilen genetik özellikleri gösterebilmektedir. Son yıllarda çevresel maruz kalma ile oluşan etkilerden korunma ya da etkinin azaltılmasında ekotoksikogenomik çalışmalar, multidisipliner kimliği ile hem erken uyarı değerlendirilmesini hem de maruz kalmanın ekosistemde oluşturduğu risklerin moleküler düzeyde etki mekanizmalarının açıklanmasını sağlayan bir bilim dalı olarak gelişmesini sürdürmektedir.

show abstract

Exploring interactions between xenobiotics, microbiota, and neurotoxicity in zebrafish

Cited by 48 publications

References 109 publications

Monoassociation with bacterial isolates reveals the role of colonization, community complexity and abundance on locomotor behavior in larval zebrafish

Monoassociation with bacterial isolates reveals the role of colonization, community complexity and abundance on locomotor behavior in larval zebrafish

Expression and Function of ABC Proteins in Fish Intestine

Ecotoxicogenomics Concept and Its Contributions to Ecological Risk Assessment

Contact Info

Product

Resources

About