Exploring MALDI-TOF MS approach for a rapid identification of<i>Mycobacterium avium</i>ssp.<i>paratuberculosis</i>field isolates

Ricchi, M.; Mazzarelli, Antonio; Piscini, Alessandro; Di, Antonino; Cannas, Angela; Leo, S.; Russo, Simone; Arrigoni, N.

doi:10.1111/jam.13357

Cited by 11 publications

(12 citation statements)

References 23 publications

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“…Although the threshold recommended by the manufacturer enabled the correct identification of only 72.1% of the isolates, a lower cut-off for mycobacteria is commonly used (Alcolea-Medina et al, 2019;Buchan, Riebe, Timke, Kostrzewa, & Ledeboer, 2014;Mediavilla-Gradolph et al, 2015;Saleeb et al, 2011). In the authors' opinion, an important drawback of the MALDI-TOF MS technology in comparison with sequence analysis of housekeeping genes such as 16S rRNA, is that yet undescribed species will go undetected, either without any identification or as misidentifi- (Ravva, Harden, & Sarreal, 2017;Ricchi et al, 2017). The 25 M. avium isolates tested in the present study were all confirmed to be Mah by sequence analysis, and the first subspecies suggestion provided by MALDI-TOF MS was generally correct (80%).…”

Section: Identification Of Mycobacteria With Maldi-tof Mass Spectrometrymentioning

confidence: 99%

Mycobacterial infections in wild boars ( Sus scrofa ) from Southern Switzerland: Diagnostic improvements, epidemiological situation and zoonotic potential

Ghielmetti

Hilbe

Friedel

et al. 2020

Transbounding Emerging Dis

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Section: Identification Of Mycobacteria With Maldi-tof Mass Spectrometrymentioning

confidence: 99%

Mycobacterial infections in wild boars ( Sus scrofa ) from Southern Switzerland: Diagnostic improvements, epidemiological situation and zoonotic potential

Ghielmetti

Hilbe

Friedel

et al. 2020

Transbounding Emerging Dis

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“…other mycobacterial groupings that were not previously possible, including Mycobacterium kansasii (2), differentiating Mycobacterium chimaera from Mycobacterium intracellulare (3), and distinguishing Mycobacterium avium subsp. paratuberculosis from other M. avium subspecies (4). In this study, we set out to examine whether MALDI-TOF MS could be used as a typing tool for outbreak investigations.…”

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confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for Investigation of Mycobacterium tuberculosis Complex Outbreaks: a Type Dream?

et al. 2020

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T he positive impact that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has had on clinical microbiology over the past decade has been well established (1). However, unfortunately, the reliable identification to the species level of Mycobacterium tuberculosis complex (MTC) isolates has not to date been achievable by MALDI-TOF MS. Nevertheless, some researchers have shown that MALDI-TOF MS coupled with in silico analysis has been useful as a typing tool for FIG 1 Summary of processing protocol for raw mass spectra and subsequent analysis, including creation of dendrograms. CWT, continuous wavelet transformation; UPGMA, unweighted pair group method with arithmetic mean; WPGMA, weighted pair group method with arithmetic mean.

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“…Ziehl-Neelsen stain-and PCR-positive colonies were then confirmed as MAP using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) [23]. Briefly, colonies were directly transferred to an MSP 384 target polished steel BC plate.…”

Section: Isolation and Identification Of Mapmentioning

confidence: 99%

Characteristics and Epidemiological Investigation of Paratuberculosis in Dairy Cattle in Tai’an, China

Cheng

Liu

Wang

et al. 2020

BioMed Research International

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Paratuberculosis, a chronic and sometimes fatal disease of ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, we examined paratuberculosis cases among 2–4-year-old dairy cows at farms in Shandong Province, China. Paratuberculosis cases were diagnosed based on clinical symptoms, pathological autopsy, and histopathological inspection. Characteristics of paratuberculosis in the affected dairy cattle included poor body condition, persistent diarrhea, subcutaneous edema, granulomatous ileitis (multibacillary), mesenteric lymphadenitis, and hepatitis. Acid-fast bacilli from fecal specimens and lymphocytes were putatively identified as MAP based on Ziehl-Neelsen staining, then confirmed using polymerase chain reaction-based testing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses. Overall, only one MAP strain was isolated from a herd with symptomatic diarrhea. However, analysis of 586 serum samples from nine herds in Tai’an City revealed that 66.7% of herds and 14.2% of animals were seropositive for MAP. Our findings suggest that paratuberculosis is widely prevalent and therefore a significant threat to the dairy industry in Tai’an City, Shandong Province, China.

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Exploring MALDI-TOF MS approach for a rapid identification ofMycobacterium aviumssp.paratuberculosisfield isolates

Cited by 11 publications

References 23 publications

Mycobacterial infections in wild boars ( Sus scrofa ) from Southern Switzerland: Diagnostic improvements, epidemiological situation and zoonotic potential

Mycobacterial infections in wild boars ( Sus scrofa ) from Southern Switzerland: Diagnostic improvements, epidemiological situation and zoonotic potential

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for Investigation of Mycobacterium tuberculosis Complex Outbreaks: a Type Dream?

Characteristics and Epidemiological Investigation of Paratuberculosis in Dairy Cattle in Tai’an, China

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