Exploring Mechanical Responses of Cells to Geometric Information Using Micropatterned DNA-Based Molecular Tension Probes

Abstract

The geometric shape of a cell is strongly influenced by the cytoskeleton, which, in turn, is regulated by integrin-mediated cell–extracellular matrix (ECM) interactions. To investigate the mechanical role of integrin in the geometrical interplay between cells and the ECM, we proposed a single-cell micropatterning technique combined with molecular tension fluorescence microscopy (MTFM), which allows us to characterize the mechanical properties of cells with prescribed geometries. Our results show that the curvature is a key geometric cue for cells to differentiate shapes in a membrane-tension- and actomyosin-dependent manner. Specifically, curvatures affect the size of focal adhesions (FAs) and induce a curvature-dependent density and spatial distribution of strong integrins. In addition, we found that the integrin subunit β 1 plays a critical role in the detection of geometric information. Overall, the integration of MTFM and single-cell micropatterning offers a robust approach for investigating the nexus between mechanical cues and cellular responses, holding potential for advancing our understanding of mechanobiology.

Cutting-Edge Research in Nanoscience and Nanotechnology: Celebrating the 130th Anniversary of Wuhan University

“…Additionally, Li and Deng created a dual growth factor release system that allows for the time-controlled release of bone morphogenetic protein 2 (BMP2) and connective tissue growth factor, providing a promising strategy for promoting bone healing . By utilizing micropatterned DNA molecular tension probes to successfully characterize the mechanical properties of cells at prescribed geometries, Liu’s group has provided a powerful method to study the relationship between mechanical cues and cellular responses . Hu et al developed a paraformaldehyde cross-linking strategy that provides a generalized strategy for studying the composition of protein crowns around intercellular nanoparticles …”

“…Advancements of nanomaterials in biorecognition, biosensing, bone defect treatment, and mechanobiology have contributed to their diversified developments in biomedical applications . To achieve prompt and reliable biofilm identification, Yuan et al developed a nanosystem of lanthanide nanoparticles with different emissions, surface charges.…”