2014
DOI: 10.1186/1754-6834-7-99
|View full text |Cite
|
Sign up to set email alerts
|

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

Abstract: BackgroundMolecular engineering of the intermediary physiology of cyanobacteria has become important for the sustainable production of biofuels and commodity compounds from CO2 and sunlight by “designer microbes.” The chemical commodity product L-lactic acid can be synthesized in one step from a key intermediary metabolite of these organisms, pyruvate, catalyzed by a lactate dehydrogenase. Synthetic biology engineering to make “designer microbes” includes the introduction and overexpression of the product-form… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

5
119
0
1

Year Published

2015
2015
2021
2021

Publication Types

Select...
5
3

Relationship

0
8

Authors

Journals

citations
Cited by 141 publications
(125 citation statements)
references
References 53 publications
5
119
0
1
Order By: Relevance
“…Synechocystis is a fully-sequenced, naturally transformable strain of cyanobacterium that is gaining popularity as a model production chassis [23]. It is currently being investigated for a variety of different products including precursors such as isoprenoids and lactic acid from CO 2 [24,25], as well as biofuels like ethanol, butanol and hydrogen .…”
Section: Case Study: Synechocystis Pcc6803mentioning
confidence: 99%
“…Synechocystis is a fully-sequenced, naturally transformable strain of cyanobacterium that is gaining popularity as a model production chassis [23]. It is currently being investigated for a variety of different products including precursors such as isoprenoids and lactic acid from CO 2 [24,25], as well as biofuels like ethanol, butanol and hydrogen .…”
Section: Case Study: Synechocystis Pcc6803mentioning
confidence: 99%
“…Entretanto, os ácidos orgânicos não são sintetizados em grande quantidade pelas cianobactérias, devido à utilização do carbono fixado para a manutenção celular (ANGERMAYR et al, 2014). Com relação aos biocombustíveis, que são uma das alternativas para suprir a escassez futura de recursos fósseis, pois são advindos de fontes renováveis e apresentam baixos custos de produção, o etanol pode ser sintetizado pelas cianobactérias, o qual é um subproduto do ciclo do Calvin (HALLENBECK, 2012).…”
Section: Introductionunclassified
“…Since Pyk is inhibited under light condition in Synechosystis, heterologous expression of Pyk can enhance the pyruvate production and in turn enhance the lactate production, where Pyk is allosterically activated by fructose 1,6-bisphosphate (FBP) in the case of Pyk-F originated form E. coli, while the original Pyk does not show such characteristics [136]. Moreover, Ppc may be knocked down to direct the carbon flow from PEP towards lactate production via PYR, but the cell growth is depressed, since Ppc is also an important pathway for CO 2 fixation [136].…”
Section: Metabolic Modification For Various Biofuel and Biochemical Pmentioning
confidence: 99%
“…Since Pyk is inhibited under light condition in Synechosystis, heterologous expression of Pyk can enhance the pyruvate production and in turn enhance the lactate production, where Pyk is allosterically activated by fructose 1,6-bisphosphate (FBP) in the case of Pyk-F originated form E. coli, while the original Pyk does not show such characteristics [136]. Moreover, Ppc may be knocked down to direct the carbon flow from PEP towards lactate production via PYR, but the cell growth is depressed, since Ppc is also an important pathway for CO 2 fixation [136]. In most bacteria, LDH requires cofactor NADH, whereas NADPH is abundant in Synechosystis as mentioned before, and thus NADPH-dependent LDH may increase the lactate production, where this may be partly attained by introducing the LDH from B. subtilis, of which LDH co-utilizes NADH and NADPH [136,137] (Table 1).…”
Section: Metabolic Modification For Various Biofuel and Biochemical Pmentioning
confidence: 99%
See 1 more Smart Citation