Exploring Potential Germline-Associated Roles of the TRIM-NHL Protein NHL-2 Through RNAi Screening

Davis, Gregory F.; Low, Wai Yee; Anderson, Joshua Wt; Boag, Peter R.

doi:10.1534/g3.117.300166

Cited by 11 publications

(7 citation statements)

References 43 publications

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“…To uncover any pathways in which NHL-2 may function to regulate germline development, we conducted a genome-wide RNAi screen for genetic interactions (Davis et al, 2017). Out of 11,511 genes screened, we identified 42 genes, that when knocked down in the nhl-2(ok818) null background resulted in strong synthetic phenotypes, including sterility, embryonic lethality and larval arrest.…”

Section: Resultsmentioning

confidence: 99%

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans

Davis

Anderson

et al. 2018

eLife

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Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

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Section: Resultsmentioning

confidence: 99%

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans

Davis

Anderson

et al. 2018

eLife

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“…In order to investigate roles of each family member, an RNAi screening approach was used where each family member was knocked down individually to investigate loss of function phenotypes (zipt 2.1, 2.2, 7.2 and 13 excluded due to technical limitations). This approach has been used to investigate the roles of individual genes or genes associated with select biological processes [29][30][31]. The development of C. elegans hermaphrodites involves an 8-h period of embryogenesis proceeded by four larval stages (L1-L4) before reaching adulthood [32].…”

Section: Zipt Family Members Contribute To Normal Lifespan In Caenorhabiditis Elegansmentioning

confidence: 99%

Zinc transporters maintain longevity by influencing insulin/IGF‐1 activity in Caenorhabditis elegans

et al. 2020

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Adequate dietary intake of essential metals such as zinc is important for maintaining homeostasis. Abnormal zinc intake in Caenorhabditis elegans has been shown to increase or decrease normal lifespan by influencing the insulin/ IGF-1 pathway. Distribution of zinc is achieved by a family of highly conserved zinc transport proteins (ZIPT in C. elegans). This study investigated the role of the zipt family of genes and showed that depletion of individual zipt genes results in a decreased lifespan. Moreover, zipt-16 and zipt-17 mutants synthetically interact with the insulin/IGF cofactors daf-16 and skn-1, and cause abnormal localisation of DAF-16. This study suggests that the zipt family of genes are required for maintaining normal lifespan through influencing the insulin/IGF-1 pathway.

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“…To uncover any pathways in which NHL-2 may function to regulate germline development, we conducted a genome-wide RNAi screen for synthetic interactions (Davis, et al, 2017). Out of 11,511 genes screened, we identified 42 genes, that when knocked down in the nhl-2(ok818) null background resulted in strong synthetic phenotypes, including sterility, embryonic lethality and larval arrest.…”

Section: Resultsmentioning

confidence: 99%

The TRIM-NHL protein NHL-2 is a Novel Co-Factor of the CSR-1 and HRDE-1 22G-RNA Pathways

Davis

et al. 2018

Preprint

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21Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. 22In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of 23 endogenous germline genes and limit the expression of deleterious transcripts to maintain genome 24 homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in 25 the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We 26 uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting 27 germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we 28 demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a 29 small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. 30

show abstract

Exploring Potential Germline-Associated Roles of the TRIM-NHL Protein NHL-2 Through RNAi Screening

Cited by 11 publications

References 43 publications

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans

The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans

Zinc transporters maintain longevity by influencing insulin/IGF‐1 activity in Caenorhabditis elegans

The TRIM-NHL protein NHL-2 is a Novel Co-Factor of the CSR-1 and HRDE-1 22G-RNA Pathways

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