Exploring Starch Sources for the Refreshment Process of Acetone-Butanol-Ethanol Fermentation with Clostridium Saccharoperbutylacetonicum N1-4

Abstract

Biobutanol is a renewable fuel that can be used as a gasoline substitute and a chemical feedstock. Its production using the Clostridial bacterial strain involves three steps: refreshment from a stock, a preculture for bacterial propagation, and primary fermentation for butanol production. Refreshment is an important process to activate the bacteria and multiply the stock. This process uses potato glucose media for C. saccharoperbutylacetonicum N1-4, while the use of starch from other sources has not been studied. This study aimed to understand various carbon sources' effects on this refreshment process as part of ABE (acetone-butanol-ethanol) fermentation. Starch was substituted in refreshment media with several types of potato, rice, sweet corn, and sweet potato at 15% w/v. After 24 hours of refreshment at an ambient temperature, fermentation was run for 48 hours in TYA (tryptone-yeast-acetate) glucose media. All the starch sources could be used in the refreshment process, resulting in butanol and total solvent concentration ranging from 7.58 to 8.76 g/L and 12.5 to 14.6 g/L, respectively. Among the samples, sweet corn provided the highest fermentation performance, with butanol of 8.76 g/L, total solvents of 14.6 g/L, average butanol productivity of 0.182 g/L/h, and a butanol yield per substrate of 0.481 C-mol/C-mol. All the starchy materials used in this experiment offered potential for ABE fermentation, while sweet corn performed remarkably-producing the highest final butanol concentration, productivity, and yield.

The Industrial Fermentation Process and Clostridium Species Used to Produce Biobutanol

“…Due to its unique characteristics, the N1-4 strain has been used in several fundamental and applied studies. These include an assessment of performance on various alternative substates with potential for industrial solvent production [75,76]. Improvements in process technology using the N1-4 strain include achieving high butanol production in fed-batch fermentations using continuous butyric acid and glucose feeding and the prolonged conversion of butyrate to butanol in a two-stage continuous culture with in situ product removal [77,78].…”

PENGARUH PERENDAMAN, WAKTU DAN KETEBALAN PADA PENGERINGAN JAHE PUTIH (Zingiber officinale var. Amarum) MENGGUNKAN TRAY DYER DAN SOLAR DRYER

“…Kadar air terendah didapatkan pada ketebalan 2 mm dan lama perendaman 25 jam yaitu sebesar 1,0785 g. Hal ini dikarenakan komponen jahe putih berinteraksi dengan larutan osmosis. Penurunan kadar air yang tinggi berhubungan dengan komponen sukrosa yang tinggi [16]- [19]. Salah satu sifat gula (sukrosa) adalah higroskopis, yaitu memiliki kemampuan dalam mengikat air bebas dalam jahe.…”

Techno-Economic Analysis of Extractive Butanol Fermentation by Immobilized Cells with Large Extractant Volume

“…The production process of butanol designed in this work is based on the recent studies [19][20][21][22]. The selected raw material is rice straw which was pretreated by low concentration of sulphuric acid to reduce inhibitor components.…”