Exploring the antimicrobial, antiviral, antioxidant, and antitumor potentials of marine Streptomyces tunisiensis W4MT573222 pigment isolated from Abu-Qir sediments, Egypt

Abstract

Due to the therapeutic importance of microbial pigments, these pigments are receiving the attention of researchers. In this present study 60 isolates were isolated from sediments of Abu-Qir coast of the Mediterranean sea, Alexandria, Egypt, out of which 12 were considered as pigmented actinomycetes. Streptomyces sp. W4 was characterized by small round green pigmented colonies when grown on starch-casein agar medium. The green pigment was extracted using a mixture of acetone-methanol (7:3 v/v). The antimicrobial, antioxidant, antiviral, and anticancer activities of the green pigment produced by Streptomyces sp.W4 were investigated. The pigment was characterized using FTIR, Raman spectroscopy, EDX and GC–MS. The results revealed that the pigment has antibacterial and antifungal activity and also showed inhibition of HAV 78% but its antiviral activity against the Adenovirus was weak. The results proved the safety of the pigment toward normal cells and anticancer activity against three different cancer cell lines HepG-2 (liver cancer cell line), A549 (lung cancer cell line), and PAN1 (pancreas cancer cell line). The pigment was combined with 9 antibiotics and then tested against the Gram-negative bacterium Enterococcus faecalis using disc diffusion bioassay. LEV showed an antagonistic effect, while CXM and CIP showed a synergistic effect.

Isolation, Characterization of Pyraclostrobin Derived from Soil Actinomycete Streptomyces sp. HSN-01 and Its Antimicrobial and Anticancer Activity

“…The red color (Figures 7 and 8) indicates the NMR signals and green color (Figure 7) represents integration value of protons. The assignment of carbons in the molecular structure was confirmed using 13 C NMR, which confirmed the presence of 19 carbon signals (Figure 8). The chemical shifts of carbons at δC 129.60 (C-8), 95.02 (C-9), and 164.28 (C-10) suggested pyrazole moiety.…”

“…Therefore, based on the obtained data from UV, FTIR, HR-ESI-MS, and NMR analyses, the structure of the compound was determined to be pyraclostrobin with the chemical formula C19H18ClN3O4 (Figure 9). The assignment of carbons in the molecular structure was confirmed using 13…”

“…Nuclear magnetic resonance (NMR) spectroscopy was performed using an FT-NMR spectrometer (400 MHz, JNM-ECZ 400S, JEOL, 11 Dearborn Road, Peabody, MA, USA). 1 H NMR and 13 C NMR spectra were recorded. Trimethylsaline was used as an internal standard, and the chemical shifts of the compound were recorded in Deuterated DMSO (DMSO-d6).…”

Bioproduction and optimization of newly characterized melanin pigment from Streptomyces djakartensis NSS-3 with its anticancer, antimicrobial, and radioprotective properties

“…Soil actinomycetes are able to produce a wide variety of products, including enzymes, bioactive secondary metabolites, melanin, and antibiotics [ 1 ] that cannot be produced by other types of bacteria because of their unique environment. Actinobacteria are highly desirable due to their potent production capacity of bioactive compounds [ 60 ].…”

“…There is a wide variety of actinomycetes, all of which are gram-positive and filamentous bacteria. They are found in natural and artificial environments and play an important role in the degradation of organic matter in soil [ 1 ]. These saprophytic, free-living bacteria are considered a major source in the development of antibiotics [ 2 , 3 ].…”