Exploring the Application of Stem Cells in Tendon Repair and Regeneration

Ahmad, Zuber; Wardale, John; Brooks, Roger A.; Henson, Frances; Noorani, Ali; Rushton, Neil

doi:10.1016/j.arthro.2011.12.009

Cited by 92 publications

(58 citation statements)

References 56 publications

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“…multipotent mesenchymal stromal cells. Mesenchymal stromal cells of various origins have been implanted directly into a tendon injury or attached to biodegradable scaffolds used to repair the tendon; they have shown an apparent beneficial effect on the healing process [4] and migrated to the injured tendon [5]. It was repeatedly shown that the delivery of mesenchymal stromal cells can create an optimal environment to support tendon tissue regeneration via the formation of extracellular matrix, enhanced vascularization, the production of supporting factors, modulation of the immunoresponse and the replacement of damaged cells [4,6].…”

Section: Introductionmentioning

confidence: 99%

Human multipotent mesenchymal stem cells improve healing after collagenase tendon injury in the rat

et al. 2014

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BackgroundMesenchymal stromal cells attract much interest in tissue regeneration because of their capacity to differentiate into mesodermal origin cells, their paracrine properties and their possible use in autologous transplantations. The aim of this study was to investigate the safety and reparative potential of implanted human mesenchymal stromal cells (hMSCs), prepared under Good Manufacturing Practice (GMP) conditions utilizing human mixed platelet lysate as a culture supplement, in a collagenase Achilles tendon injury model in rats.MethodsEighty-one rats with collagenase-induced injury were divided into two groups. The first group received human mesenchymal stromal cells injected into the site of injury 3 days after lesion induction, while the second group received saline. Biomechanical testing, morphometry and semiquantitative immunohistochemistry of collagens I, II and III, versican and aggrecan, neovascularization, and hMSC survival were performed 2, 4, and 6 weeks after injury.ResultsHuman mesenchymal stromal cell-treated rats had a significantly better extracellular matrix structure and a larger amount of collagen I and collagen III. Neovascularization was also increased in hMSC-treated rats 2 and 4 weeks after tendon injury. MTCO2 (Cytochrome c oxidase subunit II) positivity confirmed the presence of hMSCs 2, 4 and 6 weeks after transplantation. Collagen II deposits and alizarin red staining for bone were found in 6 hMSC- and 2 saline-treated tendons 6 weeks after injury. The intensity of anti-versican and anti-aggrecan staining did not differ between the groups.ConclusionshMSCs can support tendon healing through better vascularization as well as through larger deposits and better organization of the extracellular matrix. The treatment procedure was found to be safe; however, cartilage and bone formation at the implantation site should be taken into account when planning subsequent in vivo and clinical trials on tendinopathy as an expected adverse event.

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Section: Introductionmentioning

confidence: 99%

Human multipotent mesenchymal stem cells improve healing after collagenase tendon injury in the rat

et al. 2014

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“…[4][5][6][7] Consequently, attempts have been made to augment rotator cuff repairs with biologic agents. [8][9][10][11][12][13][14] Nondifferentiated progenitor cells (mesenchymal stem cells [MSCs]) can be isolated from a small volume of bone marrow aspirate and expanded in culture without undergoing differentiation. 15 These cells are capable of producing collagen in vitro, indicating their fibroblastic lineage potential.…”

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confidence: 99%

The Effect of Purified Human Bone Marrow–Derived Mesenchymal Stem Cells on Rotator Cuff Tendon Healing in an Athymic Rat

Degen

Carbone

Carballo

et al. 2016

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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“…Several animal and clinical studies have confirmed that the application of MSCs can enhance tendon repair and regeneration. 12 However, harvesting these autologous MSCs may cause injury and impairment at the donor site and potential ectopic ossification. DPSCs are easily available from discarded biological samples (such as extracted teeth) or cryopreserved dental pulp, and their self-renewal and multilineage differentiation potential provide advantages that make them a favourable option over other types of stem cells.…”

Section: Discussionmentioning

confidence: 99%

Dental pulp stem cells express tendon markers under mechanical loading and are a potential cell source for tissue engineering of tendon-like tissue

Chen

Yan

et al. 2016

Int J Oral Sci

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Postnatal mesenchymal stem cells have the capacity to differentiate into multiple cell lineages. This study explored the possibility of dental pulp stem cells (DPSCs) for potential application in tendon tissue engineering. The expression of tendon-related markers such as scleraxis, tenascin-C, tenomodulin, eye absent homologue 2, collagens I and VI was detected in dental pulp tissue. Interestingly, under mechanical stimulation, these tendon-related markers were significantly enhanced when DPSCs were seeded in aligned polyglycolic acid (PGA) fibre scaffolds. Furthermore, mature tendon-like tissue was formed after transplantation of DPSC-PGA constructs under mechanical loading conditions in a mouse model. This study demonstrates that DPSCs could be a potential stem cell source for tissue engineering of tendon-like tissue.

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Exploring the Application of Stem Cells in Tendon Repair and Regeneration

Cited by 92 publications

References 56 publications

Human multipotent mesenchymal stem cells improve healing after collagenase tendon injury in the rat

Human multipotent mesenchymal stem cells improve healing after collagenase tendon injury in the rat

The Effect of Purified Human Bone Marrow–Derived Mesenchymal Stem Cells on Rotator Cuff Tendon Healing in an Athymic Rat

Dental pulp stem cells express tendon markers under mechanical loading and are a potential cell source for tissue engineering of tendon-like tissue

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