Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition Along Different Structural Levels of Human Chorionic Gonadotropin Employing Mass Spectrometry

Lebede, Maximilian; Marco, Fiammetta Di; Esser‐Skala, Wolfgang; Hennig, René; Wohlschlager, Therese; Huber, Christian G.

doi:10.26434/chemrxiv.14153249

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…In an elegant combination of glycoproteoform profiling and noncovalent mass spectrometry, Lebede et al 387 used highresolution native MS to analyze recombinant human chorionic gonadotropin (hCG, Ovitrelle). The protein hormone hCG is involved in early embryo−maternal communication and maintenance of pregnancy.…”

Section: Human Chorionic Gonadotropin (Ovitrelle)mentioning

confidence: 99%

High-Resolution Native Mass Spectrometry

2021

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Native mass spectrometry (MS) involves the analysis and characterization of macromolecules, predominantly intact proteins and protein complexes, whereby as much as possible the native structural features of the analytes are retained. As such, native MS enables the study of secondary, tertiary, and even quaternary structure of proteins and other biomolecules. Native MS represents a relatively recent addition to the analytical toolbox of mass spectrometry and has over the past decade experienced immense growth, especially in enhancing sensitivity and resolving power but also in ease of use. With the advent of dedicated mass analyzers, sample preparation and separation approaches, targeted fragmentation techniques, and software solutions, the number of practitioners and novel applications has risen in both academia and industry. This review focuses on recent developments, particularly in high-resolution native MS, describing applications in the structural analysis of protein assemblies, proteoform profiling ofamong othersbiopharmaceuticals and plasma proteins, and quantitative and qualitative analysis of protein–ligand interactions, with the latter covering lipid, drug, and carbohydrate molecules, to name a few.

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Section: Human Chorionic Gonadotropin (Ovitrelle)mentioning

confidence: 99%

High-Resolution Native Mass Spectrometry

2021

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“…In an elegant combination of glycoproteoform profiling and non-covalent mass spectrometry, Lebede et al 387 used high-resolution native MS to analyze recombinant human chorionic gonadotropin (hCG, Ovitrelle). The protein hormone hCG is involved in early embryo-maternal communication and maintenance of pregnancy.…”

Section: Human Chorionic Gonadotropin (Ovitrelle)mentioning

confidence: 99%

Exploring Humoral Immune Responses by Mass Spectrometry

den Boer

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In his thesis “Exploring Humoral Immune Responses by Mass Spectrometry”, Maurits den Boer uses mass spectrometry to shed new light on antibody responses. Antibodies play a crucial role in the immune protection against threats like bacteria, viruses, and cancers. When valuable antibodies are discovered, they can therefore be reproduced for use as a medicine. A better understanding of their structures, interactions, and repertoires is therefore key to finding novel treatments for many diseases. In the first part of his thesis, Maurits and coworkers used mass spectrometry to study antibody structures and interactions, leading to two major findings. They first uncovered a mechanism by which Staphylococcus aureus bacteria can evade antibody responses, and how this mechanism may be circumvented in future therapies. Second, he redefined the textbook structure of circulating IgM antibodies by showing that they are universally attached to an extra protein. This may have major implications for how these antibodies function, and their use as therapeutics. In a second line of research, Maurits focused on the development of innovative techniques for antibody repertoire analysis and discovery. Together with coworkers, he explored the use of electron-based fragmentation mass spectrometry, developing methods to obtain valuable pieces of antibody sequence information. Finally, he combined multiple layers of mass spectrometry analysis to discover and fully determine the sequence of a malignant patient antibody. Combined, this demonstrates the promise of mass spectrometry as a compelling new approach for therapeutic antibody discovery.

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Exploring the Chemical Space of Protein Glycosylation in Noncovalent Protein Complexes: An Expedition Along Different Structural Levels of Human Chorionic Gonadotropin Employing Mass Spectrometry

Cited by 2 publications

References 37 publications

High-Resolution Native Mass Spectrometry

High-Resolution Native Mass Spectrometry

Exploring Humoral Immune Responses by Mass Spectrometry

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