Exploring the design implications of bacteriophages in mixed suspensions by considering attachment and break-up

Hicks, Ethan; Wiesner, Mark R.

doi:10.1016/j.watres.2022.118303

Cited by 5 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We revealed that the primary parameter is the zeta potential of polymers. This was in line with previous studies describing the heteroaggregation of phages (predominantly icosahedral, e.g., MS2) and microparticles (usually mineral or clay) using DLVO or extended DLVO theory (Chrysikopoulos and Syngouna, 2012;Armanious et al, 2016;Dang and Tarabara, 2019;Hicks and Wiesner, 2022).…”

Section: Discussionsupporting

confidence: 92%

“…forces (Hicks and Wiesner, 2022). This was in agreement with a study from 2012 by Chrysikopoulos and Syngouna.…”

Section: Introducing Physicochemical Factors Into the Modelsupporting

confidence: 92%

“…In a recent paper, Hicks and Wiesner studied bacteriophage and kaolinite heteroaggregation. Regardless of the ionic strength or the tested phage (T4) to kaolinite ratios, the phenomenon occurred rapidly and was likely driven by DLVO forces (Hicks & Wiesner, 2022). This was in agreement with a study from 2012 by Chrysikopoulos and Syngouna.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Heteroaggregation of virions and microplastics reduces the number of active bacteriophages in aqueous environments

et al. 2023

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 92%

“…forces (Hicks and Wiesner, 2022). This was in agreement with a study from 2012 by Chrysikopoulos and Syngouna.…”

Section: Introducing Physicochemical Factors Into the Modelsupporting

confidence: 92%

See 1 more Smart Citation

Heteroaggregation of virions and microplastics reduces the number of active bacteriophages in aqueous environments

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The advantage of using such a continuous system is in maintaining an adequate phage-host ratio which in turn would ensure a productive infection of the target pathogen. More importantly, this would compensate for the decay of phages and the loss of phages through hetero aggregation 20 by decoys such as sludge flocs, particulate matter and other non-targets through non-specific adsorption 38 .…”

Section: Discussionmentioning

confidence: 99%

Escherichia coli ST155 as a production-host of three different polyvalent phages and their characterisation with a prospect for wastewater disinfection

Salim

Madhavan

Subhash

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Bacteriophages are generally specific, and a cocktail of phages is needed to combat different bacterial targets. Their production usually requires pathogenic isolation hosts. We identified a novel strain, Escherichia coli ST155, that could serve as a production host for three different polyvalent phages (ϕPh_SE03, ϕPh_SD01, and ϕPh_EC01), thus superseding the use of individual isolation hosts. Upon propagation in E. coli ST155, the phages demonstrated differential intergeneric infectivity against Salmonella enterica, E. coli OP50, Shigella dysenteriae, E. coli MDR, and Acinetobacter baumannii. Phages were characterised based on morphology, latent period, burst size, the efficiency of plating, and restriction enzyme profile. Survival assay on Caenorhabditis elegans, the absence of Shiga toxin, and enterotoxigenic E. coli virulence genes indicated that E. coli ST155 could be non-pathogenic. Lack of antibiotic resistance and absence of functional prophages rendered the host suitable for environmental applications. As a proof-of-concept, phage ϕPh_SE03 was produced in ST155 by employing a unique Bacteriophage Amplification Reactor-Lytics Broadcasting System and was simultaneously disseminated into S. enterica augmented wastewater, which resulted in a 3-log reduction in 24 h. The study establishes the potential of E. coli ST155 as a phage production host thereby minimising the possibility of accidental release of pathogenic hosts into wastewater.

show abstract

“…This equation can be integrated by using a relationship for λ, which is expressed as a function of the single collector efficiency, η 0 , the attachment efficiency, the diameter of a single collector (e.g., a single glass bead), and the porosity of the porous medium. 23 Substituting for the filtration coefficient and rearranging the attachment efficiency can be expressed as a function of the experimentally observed concentrations of particles in the porous medium effluent, C and influent C 0 i k j j j j j y { z z z z z i k j j j j j y…”

Section: Calculation Of Attachment Efficiency (α)mentioning

confidence: 99%

Characterizing the Transport and Surface Affinity of Extracellular Vesicles Isolated from Yeast and Bacteria in Well-Characterized Porous Media

Rogers,

Hicks,

Kan

et al. 2023

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are membrane-bounded, nanosized particles, produced and secreted by all biological cell types. EVs are ubiquitous in the environment, operating in various roles including intercellular communication and plant immune modulation. Despite their ubiquity, the role of EV surface chemistry in determining transport has been minimally investigated. Using the zeta (ζ)-potential as a surrogate for surface charge, this work considers the deposition of EVs from the yeast, Saccharomyces cerevisiae , and two bacterial species, Staphylococcus aureus and Pseudomonas fluorescens , in well-characterized porous medium under various background conditions shown to influence the transport of other environmental colloidal particles: ionic strength and humic acid concentration. The affinity of S. cerevisiae EVs for the porous medium (glass beads) appeared to be sensitive to changes in ionic strength, as predicted by colloid stability (Derjaguin, Landau, Verwey, and Overbeek or DLVO) theory, and humic acid concentration, while P. fluorescens EVs deviated from DLVO predictions, suggesting that mechanisms other than charge stabilization may control the deposition of P. fluorescens . Calculations of attachment efficiency from these deposition studies were used to estimate EV transport using a clean-bed filtration model. Based on these calculations, EVs could be transported through such homogeneous porous media up to 15 m.

show abstract

Exploring the design implications of bacteriophages in mixed suspensions by considering attachment and break-up

Cited by 5 publications

References 37 publications

Heteroaggregation of virions and microplastics reduces the number of active bacteriophages in aqueous environments

Heteroaggregation of virions and microplastics reduces the number of active bacteriophages in aqueous environments

Escherichia coli ST155 as a production-host of three different polyvalent phages and their characterisation with a prospect for wastewater disinfection

Characterizing the Transport and Surface Affinity of Extracellular Vesicles Isolated from Yeast and Bacteria in Well-Characterized Porous Media

Contact Info

Product

Resources

About