2017
DOI: 10.1101/132084
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Exploring the Diversity ofBacilluswhole genome sequencing projects using Peasant, the Prokaryotic Assembly and Annotation Tool

Abstract: BackgroundThe persistent decrease in cost and difficulty of whole genome sequencing of microbial organisms has led to a dramatic increase in the number of species and strains characterized from a wide variety of environments. Microbial genome sequencing can now be conducted by small laboratories and as part of undergraduate curriculum. While sequencing is routine in microbiology, assembly, annotation and downstream analyses still require computational resources and expertise, often necessitating familiarity wi… Show more

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Cited by 6 publications
(6 citation statements)
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References 47 publications
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“…AF234172.1) and other Enterobacteriaceae plasmid sequences. Genome annotations were produced using the software tool Peasant ( 3 ). Eight rRNA genes, 82 tRNA genes, and 4,450 protein-coding sequences were detected.…”
Section: Genome Announcementmentioning
confidence: 99%
“…AF234172.1) and other Enterobacteriaceae plasmid sequences. Genome annotations were produced using the software tool Peasant ( 3 ). Eight rRNA genes, 82 tRNA genes, and 4,450 protein-coding sequences were detected.…”
Section: Genome Announcementmentioning
confidence: 99%
“…BLASTn queries to the NR/NT database revealed that 12 of these contigs show greatest homology to plasmid sequences of other E. faecalis strains and enterococci, including three putative complete plasmids that are 21, 31, and 35 kbp in length. Annotations were produced using the software tool Peasant ( 4 ). Six rRNAs, 52 tRNAs, and 2,852 protein-coding sequences were detected.…”
Section: Genome Announcementmentioning
confidence: 99%
“…The total genome size was 2,411,958 bp with an observed G+C content of 73.14%. Annotations were produced using the software tool Peasant ( 7 ). Five rRNAs, 48 tRNAs, and 2,317 protein-coding sequences were detected.…”
Section: Genome Announcementmentioning
confidence: 99%