Exploring the effect of a peptide additive on struvite formation and morphology: a high-throughput method

Hostert, Jacob D.; Kamlet, Olivia; Su, Zihang; Kane, Naomi S.; Renner, Julie N.

doi:10.1039/d0ra06637k

Cited by 4 publications

(17 citation statements)

References 47 publications

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“…The shADP5 peptide was shown previously to increase the formation constant and yield parameter of struvite, but binding to struvite crystals was not explored. 19 Here, we modified the peptide by capping it with a cysteine residue, C-shADP5. This peptide was then immobilized onto Au mesh and chosen due to its reliable thiol binding mechanism.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Peptides were designed based on our previous study. 19 To facilitate Au binding, a cysteine residue was placed on the N-terminus of the peptide followed by three glycine residues as spacers. The final sequence of the peptide is: CGGGSYENSH-QAINVDRT and its primary structure shown in Figure S1.…”

Section: ■ Experimental Materials and Methodsmentioning

confidence: 99%

“…Peptides were designed based on our previous study . To facilitate Au binding, a cysteine residue was placed on the N-terminus of the peptide followed by three glycine residues as spacers.…”

Section: Experimental Materials and Methodsmentioning

confidence: 99%

“…We previously conducted a small volume study showing this amelogenin peptide increases struvite yield in solution. 19 Here, we capped the peptide with cysteine (C-shADP5) to attach it to a gold (Au) substrate and investigated its ability to affect struvite formation when surface-bound. Au mesh was chosen as a model substrate to demonstrate that cysteine-capped peptides could be effectively immobilized to a surface, allowing for decreased peptide consumption and greater struvite purity compared to peptide free in solution.…”

Section: + +mentioning

confidence: 99%

“…We previously conducted a small volume study showing this amelogenin peptide increases struvite yield in solution . Here, we capped the peptide with cysteine (C-shADP5) to attach it to a gold (Au) substrate and investigated its ability to affect struvite formation when surface-bound.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Struvite Precipitation Enhanced by an Amelogenin Peptide for Nutrient Recovery

Hostert

Verma

et al. 2022

ACS Sustainable Chem. Eng.

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Precipitation of struvite (MgNH 4 PO 4 •6H 2 O) from wastewater is a promising method for recycling phosphorous, which results in a slow-release fertilizer and improves wastewater treatment costs. While chemical struvite precipitation is commercialized, electrochemical precipitation can improve the process from an energy and materials standpoint. However, new methods are needed to increase electrochemical recovery at pH values near typical wastewater conditions (6−9). Here, a surface-bound amelogenin peptide was explored in a model electrochemical system to control struvite growth at neutral pH. Molecular dynamics simulations indicated that the peptide enables favorable Mg 2+ conformations, which facilitate struvite crystallization. Chronoamperometric studies conducted with a range of immobilized peptides in synthetic wastewater at pH 7.1 produced precipitates characterized as struvite. Peptides loaded onto a gold mesh at a concentration of 19 μg mL −1 led to a 21% increase in struvite precipitation. Longer crystals formed in the presence of surface-bound peptide compared to when no peptide was present, showing that dendritic crystal growth can be achieved at neutral pH. The peptide does not appear to bind to struvite, maintaining struvite purity. Our findings show the viability of electrochemical struvite precipitation for relevant wastewater pH values and highlights how peptides can modulate crystal growth.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Experimental Materials and Methodsmentioning

confidence: 99%

Section: Experimental Materials and Methodsmentioning

confidence: 99%

Section: + +mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Electrochemical Struvite Precipitation Enhanced by an Amelogenin Peptide for Nutrient Recovery

Hostert

Verma

et al. 2022

ACS Sustainable Chem. Eng.

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Evaluating a hybrid process of anaerobic digestion, aerobic degradation, and electrochemical separation for swine wastewater treatment with methane and nutrient recovery

Chen,

Wei,

Zhong

et al. 2024

Biosystems Engineering

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Changes to Struvite Growth and Morphology as Impacted by Low Molecular Weight Organics

Rabinovich

Rouff

2023

ACS EST Water

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Dissolved organics in wastewater affect struvite (MgNH4PO4·6H2O) morphology and precipitation rates. The impact of the low molecular weight (LMW) organics benzoate (C6H5-COO–), benzylamine (C6H5-CH2-NH3 +), and toluene (C6H5-CH3 0) on struvite precipitation at constant pH and saturation was modeled in simulated wastewater (SWW). Compared to the struvite precipitation rate in LMW-free blank SWW (38.52 μmol min–1), lowest struvite precipitation rates were observed when the cationic benzylamine and nonionic toluene were present in SWW (7.19 and 16.23 μmol min–1, respectively) and the highest precipitation rate was observed in the presence of anionic benzoate (389.32 μmol min–1). Changes to struvite morphology with respect to mineral size and roundness were observed over time. Struvite collected at reaction times near maximum growth rates (60 min) was elongated with variable average sizes for benzoate, benzylamine, toluene, and blank SWW (49, 37, 32, and 14 μm, respectively). At a longer reaction time (120 min), struvite size became similar but benzoate SWW struvite was rounder than those of toluene and benzylamine SWW. Recovery of wastewater nutrients as struvite improves with better gravimetric separation for larger and rounder particles; therefore, results for benzoate treatment indicate that introducing dissolved anionic LMW organics could assist with struvite production processes.

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Exploring the effect of a peptide additive on struvite formation and morphology: a high-throughput method

Abstract: A high-throughput platform was developed to analyze struvite formation, finding that peptide addition modulates growth in a potentially favorable way.

Cited by 4 publications

References 47 publications

Electrochemical Struvite Precipitation Enhanced by an Amelogenin Peptide for Nutrient Recovery

Electrochemical Struvite Precipitation Enhanced by an Amelogenin Peptide for Nutrient Recovery

Evaluating a hybrid process of anaerobic digestion, aerobic degradation, and electrochemical separation for swine wastewater treatment with methane and nutrient recovery

Changes to Struvite Growth and Morphology as Impacted by Low Molecular Weight Organics

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