Exploring the effects of phage cocktails in preventing Vibrio infections in juvenile sea cucumber (Apostichopus japonicus) farming

Li, Zhen; Ren, Hao; Li, Qiang; Murtaza, Bilal; Li, Xiaoyü; Zhang, Jiancheng; Xu, Yongping

doi:10.1016/j.aquaculture.2019.734599

Cited by 19 publications

(6 citation statements)

References 28 publications

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“…An alternative method for targeting multiple species in a community is with polyvalent phage treatment or phage with host ranges that encompass multiple species. Descriptions of polyvalent phage have increased over the past five years likely due to directed changes in phage isolation protocols (Hamdi et al ., 2017; Duc et al ., 2020; Li et al ., 2020). In fact, Zhao and colleagues used a soil‐carrot microcosm system to compare the efficacy of a cocktail that included phage targeting two different plant pathogens with a treatment of a single polyvalent phage that infected both pathogens (Zhao et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

Phage cocktail strategies for the suppression of a pathogen in a cross‐feeding coculture

Fazzino

Anisman

Chacón

et al. 2020

Microbial Biotechnology

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Predicting which combinations (cocktails) of bacteria‐infecting viruses (bacteriophage) will suppress pathogenic bacterial growth is complicated, in part, due to the complex ecological interactions of host bacteria, but important for effective treatment. We found that when hosts cross‐feed (exchange nutrients) with nonhost bacterial species, cocktails that include a bacteriophage infecting the nonhost cross‐feeding partner can be more effective at suppressing pathogenic bacteria than targeting the pathogenic bacterium with multiple bacteriophage species. This is a novel cocktail formulation.

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Section: Discussionmentioning

confidence: 99%

Phage cocktail strategies for the suppression of a pathogen in a cross‐feeding coculture

Fazzino

Anisman

Chacón

et al. 2020

Microbial Biotechnology

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“…The addition of yeast and fermented vegetable product by Lactobacillus to the diet of Kuruma shrimp can boost the immunity resistance to V. parahaemolyticus (Elshopakey et al, 2018 ). In sea cucumber, V. alginolyticus, V. splendidus, and V. cyclitrophicus can be reduced by mixing different phage mixtures in a certain proportion, but in the experimental group treated with antibiotics, the number of Vibrio has not changed significantly (Li et al, 2020 ).…”

Section: Dietary Intervention On Vibrio Pathogenic...mentioning

confidence: 99%

Effects of intestinal microbiota on physiological metabolism and pathogenicity of Vibrio

Sun

Zhu

et al. 2022

Front. Microbiol.

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Vibrio species are disseminated broadly in the marine environment. Some of them can cause severe gastroenteritis by contaminating seafood and drinking water, such as Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus. However, their pathogenic mechanism still needs to be revealed to prevent and reduce morbidity. This review comprehensively introduces and discusses the common pathogenic process of Vibrio including adhesion, cell colonization and proliferation, and resistance to host immunity. Vibrio usually produces pathogenic factors including hemolysin, type-III secretion system, and adhesion proteins. Quorum sensing, a cell molecular communication system between the bacterial cells, plays an important role in Vibrio intestinal invasion and colonization. The human immune system can limit the virulence of Vibrio or even kill the bacteria through different responses. The intestinal microbiota is a key component of the immune system, but information on its effects on physiological metabolism and pathogenicity of Vibrio is seldom available. In this review, the effects of intestinal microorganisms and their metabolites on the invasion and colonization of common pathogenic Vibrio and VBNC status cells are discussed, which is conducive to finding the next-generation prebiotics. The strategy of dietary intervention is discussed for food safety control. Finally, future perspectives are proposed to prevent Vibrio infection in aquaculture.

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“…Studies on phage therapy have demonstrated its potential as an alternative to antibiotic therapy; for example, phages can be used to control Vibrio infection in the culture of aquatic organisms such as sea cucumbers [11,12] and Atlantic salmon [13]. Phage therapy has also been used to control the formation of Vibrio biofilms [14,15].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Novel Phage vB_VpaP_FE11 and Its Potential Role in Controlling Vibrio parahaemolyticus Biofilms

Yang

Chen

Huang

et al. 2022

Viruses

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Vibrio parahaemolyticus causes aquatic vibriosis. Its biofilm protects it from antibiotics; therefore, a new different method is needed to control V. parahaemolyticus for food safety. Phage therapy represents an alternative strategy to control biofilms. In this study, the lytic Vibrio phage vB_VpaP_FE11 (FE11) was isolated from the sewers of Guangzhou Huangsha Aquatic Market. Electron microscopy analysis revealed that FE11 has a typical podovirus morphology. Its optimal stability temperature and pH range were found to be 20–50 °C and 5–10 °C, respectively. It was completely inactivated following ultraviolet irradiation for 20 min. Its latent period is 10 min and burst size is 37 plaque forming units/cell. Its double-stranded DNA genome is 43,397 bp long, with a G + C content of 49.24% and 50 predicted protein-coding genes. As a lytic phage, FE11 not only prevented the formation of biofilms but also could destroy the formed biofilms effectively. Overall, phage vB_VpaP_FE11 is a potential biological control agent against V. parahaemolyticus and the biofilm it produces.

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Exploring the effects of phage cocktails in preventing Vibrio infections in juvenile sea cucumber (Apostichopus japonicus) farming

Cited by 19 publications

References 28 publications

Phage cocktail strategies for the suppression of a pathogen in a cross‐feeding coculture

Phage cocktail strategies for the suppression of a pathogen in a cross‐feeding coculture

Effects of intestinal microbiota on physiological metabolism and pathogenicity of Vibrio

Characterization of the Novel Phage vB_VpaP_FE11 and Its Potential Role in Controlling Vibrio parahaemolyticus Biofilms

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