Exploring the electrical wiring of screen-printed configurations utilised in electroanalysis

Galdino, Flávia Elisa; Foster, Christopher W.; Bonacin, Juliano Alves; Banks, Craig E.

doi:10.1039/c4ay02704c

Cited by 45 publications

(50 citation statements)

References 35 publications

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“…After curing at 60 degrees Celsius for 30 min the screen-printed electrode is ready to use. An edge-connector was used to ensure the reproducibility of the electrochemical connections throughout the studies [39]. Similar SPGE platforms have been electrochemically characterized in a previous contribution [40,41].…”

Section: Reagents and Chemicalsmentioning

confidence: 99%

Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples

Sanjuán

Brotons

Hernández-Ibáñez

et al. 2016

Electrochimica Acta

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Please cite this article as: Ignacio Sanjuán, Ariadna Brotons, Naiara Hernández-Ibáñez, Christopher W.Foster, Craig E.Banks, Jesús Iniesta, Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples, Electrochimica Acta http://dx.doi.org/10.1016/j.electacta. 2015.11.026 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  SWV provides good resolution of the anodic peak for 7-mG and the main nucleic bases interferences, such as adenine and guanine. It is plausible the simultaneous determination of 7-mG and another of the most common modified nucleobases, 8-oxoguanine (8-oxoG). 7-mG electrochemical determination within a human urine sample is feasible with linear regression between peak current intensity versus 7-mG concentration. 3 AbstractEpigenetic modifications have been associated by many studies with several types of diseases and metabolic dysfunctions. Specifically, N7-methyl modification of guanine (7-mG) is well established to be used as a biomarker for the detection and determination of DNA methylation. The use of an electrochemical sensor has the potential to provide a simpler and more economic sensing methodology for the determination of 7-mG compared to traditionally utilised laboratory based approaches. In this paper we demonstrate the feasibility of an electrochemical sensor which could potentially be easily applied towards the determination of 7-mG within biological samples, such as human urine. A practical electrochemical configuration was employed consisting of a boron-doped diamond electrode (BDD) as the working electrode and a screen-printed graphite electrode (SPE) providing the counter electrode and the reference electrode.With this new protocol, the electrochemical behaviour of 7-mG has been investigated via cyclic voltammetry (CV) and square wave voltammetry (SWV) using a BDD electrode with a simplified electrochemical set-up The electrochemical behaviour of 7-mG within acetate buffer solutions at a BDD electrode has been compared and contrasted to a glassy carbon electrode with the following parameters studied: voltammetric scan rate, solution pH, 7-mG concentration and electrode surface pretreatment. The oxidative mechanism elucidation has been performed at controlled potential and such results have provided the dimer formation as the major product. The simultaneous electroanalytical identification of 7-mG together with the presence of guanine, adenine and 8-oxoguanine has been investigated under the optimum experimental conditions. Furthermore, the feasibility of using a BDD electrode for the ...

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Section: Reagents and Chemicalsmentioning

confidence: 99%

Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples

Sanjuán

Brotons

Hernández-Ibáñez

et al. 2016

Electrochimica Acta

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“…All measurements were performed at 25˝C. In addition an edge-connector was used to ensure the reproducibility of the electrochemical connections throughout the studies [61].…”

Section: Electrochemical Measurementsmentioning

confidence: 99%

“…Last a dielectric paste ink (Product Code: D2070423P5; Gwent Electronic Materials Ltd., Pontypool, UK) was printed to cover the connections and define the 3 mm diameter graphite working electrode. After curing at 60˝C for 30 min the screen-printed electrode was ready to use [61].…”

Section: Electrochemical Measurementsmentioning

confidence: 99%

High Yield Synthesis of Hydroxyapatite (HAP) and Palladium Doped HAP via a Wet Chemical Synthetic Route

et al. 2016

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Abstract:A novel procedure for the synthesis of both hydroxyapatite (HAP) and palladium doped HAP via a wet chemical precipitation method is described herein. X-ray Diffraction (XRD), Raman Spectroscopy, Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS) and Fourier Transform Infrared (FT-IR) Spectroscopy are utilised to characterise the synthesised material's morphology, structure and crystallinity. The developed synthetic protocol produces high purity HAP with an average yield of 83.7 (˘0.10)% and an average particle size of 58.2 (˘0.98) nm, such synthesis has been achieved at room temperature and within a time period of less than 24 h. Additionally, in order to enhance the overall conductivity of the material, a range of Pd (2, 4 and 6 wt %) metal doped HAP has been synthesised, characterised and, for the first time, applied towards the competitive electrocatalytic detection of hydrazine, exhibiting a linear range of 50-400 µM with a limit of detection (3σ) of 30 µM.

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“…electrode is ready to use. An edge-connector was used to ensure the reproducibility of the electrochemical connections throughout the studies (Galdino et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Hernández-Ibáñez

García‐Cruz

Montiel

et al. 2016

Biosensors and Bioelectronics

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Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures, Biosensors and Bioelectronic, http://dx.doi.org/10.1016/j.bios.2015.11.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. This novel electrochemical lactate biosensors analytical efficacy is explored towards the sensing of lactate in model (buffer) solutions and is found to exhibit a linear response towards lactate over the concentration range of 30.4 and 243.9 µM in phosphate buffer solution, with a corresponding limit of detection (based on 3-sigma) of 22.6 µM and exhibits a sensitivity of 3417 ± 131 µA M -1 according to the reproducibility study. These novel electrochemical lactate biosensors exhibit a high reproducibility, with a relative standard deviation of less than 3.8 % and an enzymatic response over 82 % after 5 months stored at 4 ºC. Furthermore, high performance liquid chromatography technique has been utilized to independently validate the electrochemical lactate biosensor for the determination of lactate in a commercial embryonic cell culture medium providing excellent agreement between the two analytical protocols.

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Exploring the electrical wiring of screen-printed configurations utilised in electroanalysis

Cited by 45 publications

References 35 publications

Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples

Boron-doped diamond electrodes explored for the electroanalytical detection of 7-methylguanine and applied for its sensing within urine samples

High Yield Synthesis of Hydroxyapatite (HAP) and Palladium Doped HAP via a Wet Chemical Synthetic Route

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

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