Exploring the Genetic Cipher of Chickpea (Cicer arietinum L.) Through Identification and Multi-environment Validation of Resistant Sources Against Fusarium Wilt (Fusarium oxysporum f. sp. ciceris)

Sharma, Mamta; Ghosh, Raju; Tarafdar, Avijit; Rathore, Abhishek; Chobe, Devashish R.; Kumar, Anil; Gaur, Pooran M.; Srinivasan, S.; Gupta, O. P.; Singh, Narendra Pratap; Saxena, D. R.; Saifulla, Muhammad; Pithia, M. S.; Ghante, P.H.; Mahalinga, Deyanand M.; Upadhyay, Jharana; Harer, P. N.

doi:10.3389/fsufs.2019.00078

Cited by 43 publications

(17 citation statements)

References 27 publications

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“…Discrimination and representativeness analyses reveal that E6 (Nandyal) is the most representative location. The genotypes were highly stable in this location, as was reported by Sharma et al (2019) while screening for wilt-resistant genotypes in wilt sick plot over ten locations. The top three best performing introgression lines (IL1, IL4 and IL3) with more than 30% yield advantage over recurrent parent Pusa 391 were nominated for AVT trials based on the multi-location studies.…”

Section: Discussionsupporting

confidence: 75%

Development of High Yielding Fusarium Wilt Resistant Cultivar by Pyramiding of “Genes” Through Marker-Assisted Backcrossing in Chickpea (Cicer arietinum L.)

et al. 2022

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Pusa 391, a mega desi chickpea variety with medium maturity duration is extensively cultivated in the Central Zone of India. Of late, this variety has become susceptible to Fusarium wilt (FW), which has drastic impact on its yield. Presence of variability in the wilt causing pathogen, Fusarium oxysporum f.sp. ciceri (foc) across geographical locations necessitates the role of pyramiding for FW resistance for different races (foc 1,2,3,4 and 5). Subsequently, the introgression lines developed in Pusa 391 genetic background were subjected to foreground selection using three SSR markers (GA16, TA 27 and TA 96) while 48 SSR markers uniformly distributed on all chromosomes, were used for background selection to observe the recovery of recurrent parent genome (RPG). BC1F1 lines with 75–85% RPG recovery were used to generate BC2F1. The plants that showed more than 90% RPG recovery in BC2F1 were used for generating BC3F1. The plants that showed more than 96% RPG recovery were selected and selfed to generate BC3F3. Multi-location evaluation of advanced introgression lines (BC2F3) in six locations for grain yield (kg/ha), days to fifty percent flowering, days to maturity, 100 seed weight and disease incidence was done. In case of disease incidence, the genotype IL1 (BGM 20211) was highly resistant to FW in Junagarh, Indore, New Delhi, Badnapur and moderately resistant at Sehore and Nandyal. GGE biplot analysis revealed that IL1(BGM20211) was the most stable genotype at Junagadh, Sehore and Nandyal. GGE biplot analysis revealed that IL1(BGM 20211) and IL4(BGM 20212) were the top performers in yield and highly stable across six environments and were nominated for Advanced Varietal Trials (AVT) of AICRP (All India Coordinated Research Project on Chickpea) in 2018–19. BGM20211 and BGM 20212 recorded 29 and 28.5% average yield gain over the recurrent parent Pusa 391, in the AVT-1 and AVT-2 over five environments. Thus, BGM20211 was identified for release and notified as Pusa Manav/Pusa Chickpea 20211 for Madhya Pradesh, Gujarat and Maharashtra, Southern Rajasthan, Bundhelkhand region of Uttar Pradesh states by the Central Sub-Committees on Crop Standards, Notification and Release of Varieties of Agricultural Crops, Ministry of Agriculture and Farmers Welfare, Government of India, for commercial cultivation in India (Gazette notification number S.O.500 (E) dt. 29-1-2021).Such pyramided lines give resilience to multiple races of fusarium wilt with added yield advantage.

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Section: Discussionsupporting

confidence: 75%

Development of High Yielding Fusarium Wilt Resistant Cultivar by Pyramiding of “Genes” Through Marker-Assisted Backcrossing in Chickpea (Cicer arietinum L.)

et al. 2022

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“…Elements of climatic change are one of the key factors influencing the epidemiology of plant diseases (Graham and Vance, 2003;Zhao and Running, 2010;Sharma et al, 2019), as the life cycle of phytopathogens is heavily dependent on weather components such as temperature, rainfall, soil moisture, humidity, and greenhouse gases (IPPC, 2007;Sharma et al, 2015). Thus, to have a clear understanding of the host-pathogen interaction at different planes, viz., cellular, physiological, and molecular levels, it is imperative that we study plant diseases in a combined stress scenario having the interaction of both biotic and abiotic stresses, rather than its individual counterparts.…”

Section: Introductionmentioning

confidence: 99%

Temperature and Soil Moisture Stress Modulate the Host Defense Response in Chickpea During Dry Root Rot Incidence

et al. 2021

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Dry root rot caused by the necrotrophic phytopathogenic fungus Rhizoctonia bataticola is an emerging threat to chickpea production in India. In the near future, the expected increase in average temperature and inconsistent rainfall patterns resultant of changing climatic scenarios are strongly believed to exacerbate the disease to epidemic proportions. The present study aims to quantify the collective role of temperature and soil moisture content (SMC) on disease progression in chickpea under controlled environmental conditions. In our study, we could find that both temperature and soil moisture played a decisive role in influencing the dry root rot disease scenario. As per the disease susceptibility index (DSI), a combination of high temperature (35°C) and low SMC (60%) was found to elicit the highest disease susceptibility in chickpea. High pathogen colonization was realized in chickpea root tissue at all time-points irrespective of genotype, temperature, and SMC. Interestingly, this was in contrast to the DSI where no visible symptoms were recorded in the roots or foliage during the initial time-points. For each time-point, the colonization was slightly higher at 35°C than 25°C, while the same did not vary significantly with respect to SMC. Furthermore, the differential expression study revealed the involvement of host defense-related genes like endochitinase and PR-3-type chitinase (CHI III) genes in delaying the dry root rot (DRR) disease progression in chickpea. Such genes were found to be highly active during the early stages of infection especially under low SMC.

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“…Since Fusarium is soil-borne pathogen, pot evaluation is considered efficient and accurate as it takes less space, provides uniform inoculum load and limits interaction with other soil-borne pathogens such as Rhizoctonia bataticola and Sclerotium rolfsii causing synergistic effect [27]. To increase the efficiency and reduce variation of screening the germplasms were screened for two consecutive seasons, 2020 and 2021 [28]. The germplasm showed typical wilt symptoms, yellowing, drooping to wilting of the plant followed by death.…”

Section: Discussionmentioning

confidence: 99%

Resistance screening and in-silico characterization of cloned novel RGA from multi race resistant Lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

Nishmitha

Singh

Dubey

et al. 2022

Preprint

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Fusarium wilt caused by Fusarium oxysporum f. sp. lentis (Fol) is the most devastating disease of lentil present worldwide and in India. Identification of multi-race fusarium wilt resistance genes and incorporation into existing cultivar will help to reduce yield loss. In the present study, a hundred lentil germplasm were screened against seven prevalent races of Fol and accession IC201561, EC714243 and EC718238 were identified resistant. The typical R gene codes for the nucleotide-binding site and leucine-rich repeats (NBS-LRR) at the C terminal linked to either Toll/interleukin 1- like receptor (TIR) or coiled-coil (CC) at the N terminal. In the present study degenerate primers designed from the NBS region amplifying P-loop to GLPLA motif isolated forty-five resistance gene analogues (RGA) from identified resistant accessions. The sequence alignment identified both classes of RGA, TIR and non-TIR based on the presence of Aspartate (D) and Tryptophan (W) at the end of kinase motif respectively. The phylogenetic analysis grouped RGA into six classes, LRGA1 to LRGA6 determining the diversity of RGA present in the host. Grouping of RGA identified from Lens nigricans, LnRGA 2, 9, 13 with I2 reveals a probable role in Fusarium resistance. The similarity index of 27.85% to 86.98% was found among RGA and 26.83% to 49.41% between known R genes, I2, Gpa2, M and L6. Active binding sites present along the conserved motifs have grouped the RGA into 13 groups. ADP/ATP being the potential ligand determines ATP binding and ATP hydrolysis activity of RGA. The isolated RGA can be used in developing marker linked to the functional R gene. Further, expression analysis and full-length gene isolation further pave path to identifying the molecular mechanism involved in resistance.

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Exploring the Genetic Cipher of Chickpea (Cicer arietinum L.) Through Identification and Multi-environment Validation of Resistant Sources Against Fusarium Wilt (Fusarium oxysporum f. sp. ciceris)

Cited by 43 publications

References 27 publications

Development of High Yielding Fusarium Wilt Resistant Cultivar by Pyramiding of “Genes” Through Marker-Assisted Backcrossing in Chickpea (Cicer arietinum L.)

Development of High Yielding Fusarium Wilt Resistant Cultivar by Pyramiding of “Genes” Through Marker-Assisted Backcrossing in Chickpea (Cicer arietinum L.)

Temperature and Soil Moisture Stress Modulate the Host Defense Response in Chickpea During Dry Root Rot Incidence

Resistance screening and in-silico characterization of cloned novel RGA from multi race resistant Lentil germplasm against Fusarium wilt (Fusarium oxysporum f. sp. lentis)

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