Exploring the influence of a XeCl laser treatment on biocompatibility of polyethersulfone film

Abstract

The effect of XeCl laser irradiation on biocompatibility of polyethersulfone (PES) film surface was investigated. For this purpose, the surface of PES film was irradiated with different number of pulses at dif ferent fluences. The treated surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM) and contact angle measurements. The platelet adhesion and cell culture measurements were done on the treated surface for investigation of the biocompatibility. It was shown that, irradiation of surface with 500 pulses at a fluence of 25 mJ/cm 2 is the most optimal condition for improving the platelet adhesion on the PES surface with a XeCl laser.

Laser surface modification of polyethersulfone films: effect of laser wavelength on biocompatibility

“…The most optimal condition for decreasing platelet adhesion on the surface is a fluence of 25 mJ/cm 2 and 500 pulses ( fig.4). On the other hand with irradiation of surface with 500 pulses at a fluence of 70 mJ/cm 2 the contact angle increaseswhile the number of adhered platelet decreases [16]. Results of the cell culture studies show that fewer cells areattached on untreated surface in comparison to the laser treated ones.…”

“…Two factors should be considered for cell adhesion on an irradiated surface: 1-chemical composition and 2-roughnessdue to structure formation or debris [16,25]. According tothe contact angle measurements, it seems below the ablation threshold; chemical changes of the polymer surface including oxidization in ambient air and creation of polar groups lead to increased cell adhesion [19,25].…”

“…PES films were prepared as described elsewhere [16][17][18][19][20][21]. They were cut into pieces of 80 100 m in thickness and 30mm 10mm in dimension and were washed with ethanol before irradiation.…”

“…The number of adhered platelets was determined by LDH (lactate dehydrogenase) method [16,17]; and cell culture studies were done using L929 mouse cells for investigation of the surface toxicity [16,19]. …”

“…In our last papers [16][17][18][19][20][21] we investigated the effect of KrF, XeCl and ArF laser irradiation on the surface chemical,morphological and wettability of the polyethersulfone surface, individually. In this paper a brief comparison review between different wavelengths is done.…”