Exploring the mechanism of fluorescence quenching in two biologically active boronic acid derivatives using Stern-Volmer kinetics

Geethanjali, H.S.; Nagaraja, D.; Melavanki, Raveendra

doi:10.1016/j.molliq.2015.06.025

Cited by 51 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The decay profile is as shown Figure a. The S–V plots of I 0 /I against [ Q ] for the solute in all solvents are found to be nonlinear as shown in Figure b …”

Section: Methodsmentioning

confidence: 81%

“…Figure b clearly indicates that the phenomenon of fluorescence quenching, in steady state methods follows the upward curvature Stern–Volmer equation. Possibility of static quenching through ground state complex formation is evaluated using modified S–V equation . In present study K SV obtained from extended S–V equation is imaginary involving complex number.…”

Section: Resultsmentioning

confidence: 97%

“…In systems even when there is no stable ground‐state complex formation substantial upward curvature is accounted by sphere of action static quenching model along with the dynamic quenching. According to static quenching model, straight line plot is obtained for [1 − ( I / I O )]/[ Q ] against I / I O and is shown in Figure a for 2BPBA for all solvents . Magnitudes of static quenching constant V , W , and radii r of sphere of action are determined.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, study of fluorescence quenching has attracted many researchers due to its many novel applications in emerging fields of science and technology . Among various experimental techniques, determination of the quenching parameters using Stern–Volmer (S–V) plot is commonly used to study the fluorescence quenching . Due to the interesting properties associated with boronic acids, we undertook the fluorescence quenching studies of newly synthesized boronic acid dye.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of Contact and Collisional Quenching Model on 2‐Bromophenyl Boronic Acid Using Positive Deviation S–V Plots

Sharma

Patil²,

Melavanki

et al. 2019

Macromolecular Symposia

View full text Add to dashboard Cite

The steady state studies of fluorescence quenching of excitation energy of heterocyclic compound namely 2‐bromophenyl boronic acid (2BPBA) by aniline was carried out in 1, 4 dioxane (DX), toluene (TL), methanol (ML), heptane (HP), nonane (NN), butanol (BL), and propanol (PL) solvents with a view to understand the role of quenching mechanism. The quenching process is studied in the selected solvents using steady state method by Stern–Volmer (S–V) plots having upward curvature.

show abstract

“…The decay profile is as shown Figure a. The S–V plots of I 0 /I against [ Q ] for the solute in all solvents are found to be nonlinear as shown in Figure b …”

Section: Methodsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Contact and Collisional Quenching Model on 2‐Bromophenyl Boronic Acid Using Positive Deviation S–V Plots

Sharma

Patil²,

Melavanki

et al. 2019

Macromolecular Symposia

View full text Add to dashboard Cite

show abstract

“…Nevertheless, fluorescent compound and quencher must be in contact for both type of quenching. The Stern-Volmer plots are individually linear for both quenching mechanisms, which indicates that only one type of quenching occurs (Lakowicz, 2006;Geethanjali et al, 2015). However, when both quenching mechanisms exist simultaneously, non-linearities may be observed (Lakowicz, 2006;Thipperudrappaa et al, 2007).…”

Section: Quenching Interaction Between Bod-3 and O-bbvmentioning

confidence: 94%

Fluorescence quenching-based bodipy-boronic acid linked viologen dual system for potential glucose sensing applications

Topel

Beyaz

2021

View full text Add to dashboard Cite

Purpose The purpose of this study is to develop a non-enzymatic based glucose-sensing platform composed of Bodipy-BBV dual system which can be monitored by a photodetector under the blue LED excitation. Design/methodology/approach The sensor has been developed from a dual system including a fluorescent dye, an aldehyde derivative of boron dipyrromethene (Bodipy) and a quencher, orto-boronic acid linked viologen (o-BBV) where their combination resulted in a ratiometric fluorescence quenching in ethanol: PBS (1:1, pH:7.4) solution under UV light excitation. By glucose addition, o-BBV has been released from the Bodipy and binded to cis-diol groups of glucose, thereby fluorescence emission of Bodipy has been regained. Furthermore, a setup consisting of a light emitting diode (LED) and a photodiode (PD) was used to prove electrical detection of glucose without the need for expensive and bulky optical equipment, enabling the development of a miniaturized and low-cost glucose-sensing platform. Findings The fluorescence intensity of the Bodipy derivative in the solution (2 × 10−6 M) was diminished by 93% in the presence of o-BBV solution (5 × 10−3 M). Upon the glucose addition, 81% of the Bodipy fluorescence intensity has been recovered after introduction of 30 mM of glucose, where the ratio of o-BBV/Bodipy was 35:1. A linear response between 10 and 30 mM glucose concentration was obtained, which covers the biologically significant range. A high correlation between the photodiode current and Bodipy fluorescence intensity was achieved. Originality/value Even though Bodipy molecules are known with their superior optical properties and applied to the fluorescence-based detection of glucose, to the best of the authors’ knowledge, no work has been reported on Bodipy-BBV dual system to detect glucose molecules as a non-enzymatic based method. This design enables the dye and the quencher to independently coexist in the solution, allowing for tuning of their individual concentrations to optimize the glucose sensitivity. Furthermore, an electrical light detection scheme consisting of a LED and a photodiode has been implemented to eliminate the bulky optical equipment from the measurement setup and further this work for the development of a compact and inexpensive sensor. The results presented here demonstrate the feasibility of this system for the development of a novel glucose sensor.

show abstract

pH‐responsive zwitterionic carbon dots for detection of rituximab antibody

Emami

Mousazadeh

2021

Luminescence

View full text Add to dashboard Cite

Zwitterionic carbon dots (CDs) have received much attention as a result of good photostability, high biocompatibility, and high quantum yield. In this study, novel zwitterionic CDs were synthesized using a simple hydrothermal method of citric acid (CA) and l‐histidine as carbon and nitrogen precursors, respectively. Prepared zwitterionic CDs have an average particle size of 4 nm diameter and showed green fluorescence with a peak at 530 nm when excited at 470 nm; quantum efficiency was 39.34% using rhodamine 6G as a baseline. The fluorescence intensity of zwitterionic CDs was quenched by rituximab in the range 0–400 μmol L−1, with a limit of detection of 27 μmol L−1. In addition, the synthesized zwitterionic CDs had low toxicity, good stability, and high selectivity and sensitivity sensing for rituximab, therefore zwitterionic CDs are a promising candidate for practical applications.

show abstract

Exploring the mechanism of fluorescence quenching in two biologically active boronic acid derivatives using Stern-Volmer kinetics

Cited by 51 publications

References 35 publications

Effect of Contact and Collisional Quenching Model on 2‐Bromophenyl Boronic Acid Using Positive Deviation S–V Plots

Effect of Contact and Collisional Quenching Model on 2‐Bromophenyl Boronic Acid Using Positive Deviation S–V Plots

Fluorescence quenching-based bodipy-boronic acid linked viologen dual system for potential glucose sensing applications

pH‐responsive zwitterionic carbon dots for detection of rituximab antibody

Contact Info

Product

Resources

About