Exploring the Potential of 1-Pentanol and Oleic Acid for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Copolymer by Cupriavidus sp. USMAA1020

Huong, Kai‐Hee; Shantini, K.; Sharmini, R.; Amirul, Al-Ashraf Abdullah

doi:10.1007/s13369-017-2473-7

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…Moreover, the 0.01 wt% increase of 1-pentanol supply, as suggested by the software, had accumulated copolymer with approximately twofold increment of 3HV monomer composition from 4 to 7 mol%. However, in this study, the 3HV monomer composition obtained achieved a two-fold increase that was higher than the result obtained by Huong and coworkers who observed a decrease in 3HV monomer composition after RSM optimization (Huong et al, 2017).…”

Section: Optimization Of P(3hb-co-3hv) Production Using the Statisticcontrasting

confidence: 89%

“…The use of a common 3HV precursor, valeric acid, contributed to the highest 3HV monomer composition (91 ± 1 mol%) incorporated in the P(3HB) homopolymer compared with other 3HV precursors (Table 2). This might be influenced by the alternative pathway that converted valeric acid to valery-CoA by an enzyme known as acyl-CoA synthase (Huong et al, 2017). Based on similar reports, valeryl-CoA was further converted into the (R)-3hydroxyvaleryl-CoA and finally converted to the copolymeric 3HV monomer unit.…”

Section: Effect Of Various 3hv Precursors On P(3hb-co-3hv) Productionmentioning

confidence: 94%

“…A differential scanning calorimetry (DSC) machine (Perkin Elmer, Massachusetts, USA) was used to analyze the thermal properties (Huong et al, 2017). An aluminium pan was used to enclose -5-8 mg of polymer film.…”

Section: Mechanical and Thermal Properties Of P(3hb-co-3hv)mentioning

confidence: 99%

“…This strain, cultivated using 1-pentanol (3HV precursor) and glucose as the carbon sources, had achieved the highest P(3HB-co-3HV) content at 45 ± 2 wt% with a copolymer concentration of 1.30 ± 0.20 g/l. This is likely caused by the alcohol's relatively lower toxicity toward bacterial cells compared to other 3HV precursors (Shantini et al, 2013;Huong et al, 2017). For example, the use of 1-pentanol with oleic acid resulted in the production of up to 4.5 ± 0.3 g/l with 8 mol% of 3HV monomer by Cupriavidus sp.…”

Section: Effect Of Various 3hv Precursors On P(3hb-co-3hv) Productionmentioning

confidence: 99%

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Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Kiun¹,

Amelia²,

Huong³

et al. 2019

bta

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Polyhydroxyalkanoate (PHA) is a microbial storage polymer that is naturally produced by certain bacteria. This is the first study on the ability of this particular species Massilia haematophila to synthesize a PHA copolymer containing 3-hydroxyvalerate (3HV) monomer. Using the statistical design on Massilia haematophila UMTKB-2, this study highlights the optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), copolymer production for shaken-flask cultivation. Moreover, the mechanical and thermal features of the polymers were determined. The production of P(3HB-co-3HV) by Massilia haematophila UMTKB-2 using optimal conditions provided by response surface methodology (RSM) yielded 5.0 g/l of P(3HB-co-7 mol% 3HV), which was higher than the value obtained from unoptimized conditions such as 4.40 g/l of P(3HB-co-4mol% 3HV). This result showed a 14% increase in copolymer concentration and a twofold increase in 3HV composition. In this study, the P(3HB-co-3HV) synthesized was determined as a block copolymer and its thermal properties were better than P(3HB). Using RSM, the optimization conditions were successfully obtained for this bacterium, and this result is a starting platform for additional studies of a larger scaled PHA production from Massilia haematophila UMTKB-2 using bioreactors.

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Section: Optimization Of P(3hb-co-3hv) Production Using the Statisticcontrasting

confidence: 89%

Section: Effect Of Various 3hv Precursors On P(3hb-co-3hv) Productionmentioning

confidence: 94%

Section: Mechanical and Thermal Properties Of P(3hb-co-3hv)mentioning

confidence: 99%

Section: Effect Of Various 3hv Precursors On P(3hb-co-3hv) Productionmentioning

confidence: 99%

See 2 more Smart Citations

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Kiun¹,

Amelia²,

Huong³

et al. 2019

bta

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“…C. malaysiensis USMAA2-4, C. malaysiensis USMAA1020, and C. malaysiensis USMAA9-39 are three PHA-producing bacteria favoring alkyl alcohols over organic acids for 3HV formation [123]. C. malaysiensis USMAA2-4 and C. malaysiensis USMAA1020 were able to accumulate 7-10 mol% 3HV from 1-pentanol [87,88,[123][124][125] (Table 1). The 3HV yield of C. malaysiensis USMAA2-4 and its transformant strain harboring C. necator H16 lipAB genes was 0.22 g/g and 0.33 g/g, respectively, which were both higher than 0.14 g/g for C. necator H16 [13,87,88].…”

Section: Alkyl Alcohol-tolerant P(3hb-co-3hv)-producing Bacteriamentioning

confidence: 99%

A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) Production from Alkyl Alcohols

2022

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)) is the most studied short-chain-length polyhydroxyalkanoates (PHA) with high application importance in various fields. The domination of high-cost propionate and valerate over other 3-hydroxyvalerate (3HV) precursors owing to their wide preference among PHA-producing bacteria has hindered the development of diverse production processes. As alkyl alcohols are mainly produced from inexpensive starting materials through oxo synthesis, they contribute a cost-effective advantage over propionate and valerate. Moreover, alkyl alcohols can be biosynthesized from natural substrates and organic wastes. Despite their great potential, their toxicity to most PHA-producing bacteria has been the major drawback for their wide implementation as 3HV precursors for decades. Although the standard PHA-producing bacteria Cupriavidus necator showed promising alcohol tolerance, the 3HV yield was discouraging. Continuous discovery of alkyl alcohols-utilizing PHA-producing bacteria has enabled broader choices in 3HV precursor selection for diverse P(3HB-co-3HV) production processes with higher economic feasibility. Besides continuous effort in searching for promising wild-type strains, genetic engineering to construct promising recombinant strains based on the understanding of the mechanisms involved in alkyl alcohols toxicity and tolerance is an alternative approach. However, more studies are required for techno-economic assessment to analyze the economic performance of alkyl alcohol-based production compared to that of organic acids.

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Biotransformation of oleochemical industry by‐products to polyhydroxyalkanoate bioplastic using microbial fermentation: A review

Kakar

Amelia

Teng

et al. 2021

Environmental Quality Mgmt

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For many decades, global efforts have been attempted to replace the petrochemical plastics with biodegradable and environment friendly plastics. The petrochemical plastics that require many years to decompose have profound consequences on the environment. A natural, biodegradable, and plastic‐like polymer, polyhydroxyalkanoate (PHA), has been extracted from and synthesized by microbes, particularly bacteria. However, the prospective large‐scale production of PHA faced an economic bottleneck for less production costs. Research has thus shifted to one of the most common alternatives, which is the utilization of less costly raw materials or carbon sources that include industrial by‐products and wastes, such as glycerine pitch and glycerol. This review elucidates the discovery, basic mechanisms, and applications of PHA, as well as provides insights regarding the importance of PHA conversion from glycerine pitch and glycerol. This review also summarizes some possible future modifications to improve the efficiency of glycerine pitch and glycerol as carbon sources in PHA production. The conversion endeavors of wastes to a valuable product, PHA, that not only reduces petrochemical plastic pollution, waste generation, and PHA production costs but also supports economic and resource sustainability, which ultimately further progress towards the cradle‐to‐cradle design and circular bioeconomy concept.

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Exploring the Potential of 1-Pentanol and Oleic Acid for Optimizing the Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Copolymer by Cupriavidus sp. USMAA1020

Cited by 8 publications

References 18 publications

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-co-3-hydroxyvalerate) Production from Alkyl Alcohols

Biotransformation of oleochemical industry by‐products to polyhydroxyalkanoate bioplastic using microbial fermentation: A review

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