Exploring the Potential of Biocompatible Osmoprotectants as Highly Efficient Cryoprotectants

Yang, Jing; Pan, Chao; Zhang, Jiamin; Sui, Xiaojie; Zhu, Yingnan; Wen, Chiyu; Zhang, Lei

doi:10.1021/acsami.7b12189

Cited by 69 publications

(98 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within cold-tolerant organisms, the function of AFPs combined with different organic and inorganic materials, including glycerol, glucose, ions, lipids, mineral salts, peptides, and carbohydrates. Therefore, utilizing optimal combinations of these substances can result in the maximized AFP performance differing between the usages [ 80 , 81 ].…”

Section: Discussionmentioning

confidence: 99%

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture

et al. 2020

View full text Add to dashboard Cite

Antifreeze proteins (AFPs) are specific proteins, glycopeptides, and peptides made by different organisms to allow cells to survive in sub-zero conditions. AFPs function by reducing the water’s freezing point and avoiding ice crystals’ growth in the frozen stage. Their capability in modifying ice growth leads to the stabilization of ice crystals within a given temperature range and the inhibition of ice recrystallization that decreases the drip loss during thawing. This review presents the potential applications of AFPs from different sources and types. AFPs can be found in diverse sources such as fish, yeast, plants, bacteria, and insects. Various sources reveal different α-helices and β-sheets structures. Recently, analysis of AFPs has been conducted through bioinformatics tools to analyze their functions within proper time. AFPs can be used widely in various aspects of application and have significant industrial functions, encompassing the enhancement of foods’ freezing and liquefying properties, protection of frost plants, enhancement of ice cream’s texture, cryosurgery, and cryopreservation of cells and tissues. In conclusion, these applications and physical properties of AFPs can be further explored to meet other industrial players. Designing the peptide-based AFP can also be done to subsequently improve its function.

show abstract

Section: Discussionmentioning

confidence: 99%

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Proline and glycine are two types of representative osmoprotectants (Yang et al, 2017). Betaine, the metabolite of glycine, also acts as an effective osmoprotectant in Lactobacillus salivarius and Lactobacillus buchneri (Sheehan et al, 2006;Louesdon et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Pathway Profiling in Intracellular and Extracellular Environments of Streptococcus thermophilus During pH-Controlled Batch Fermentations

Qiao

Liu

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Elucidating the metabolite profiles during the growth of Streptococcus thermophilus is beneficial for understanding its growth characteristics. The changes in the intracellular and extracellular concentrations of carbohydrates, nucleotides, amino sugars, nucleoside sugars, fatty acids, and amino acids, as well as their metabolites over time, were investigated by metabolomics technology. Most metabolites of nucleotides were highly accumulated in the intracellular environment after the mid-exponential phase. Increases in the intracellular unsaturated fatty acids and N-acetyl-glucosamine and N-acetyl-muramoate recycling provided potential evidence that cell envelope remodeling occurred after the mid-exponential phase. At the later fermentation stages, potentially functional metabolite produced by glycine was highly accumulated in the intracellular environment. Additionally, potential toxic metabolites produced by phenylalanine and tyrosine could not be excreted into the extracellular environment in a timely basis. The accumulation of large amounts of these metabolites might be the primary cause of the overconsumption of amino acids and influence the growth of S. thermophilus.

show abstract

“…The freeze concentration-induced excessive dehydration can damage cells resulting in solution injury. Besides this, ice formation and growth during cryopreservation can mechanically damage the biological structure of cells resulting in ice injury [4] (Fig. 1a).…”

Section: Introductionmentioning

confidence: 99%

Advanced Biotechnology for Cell Cryopreservation

Yang

Gao

Liu

et al. 2019

Trans. Tianjin Univ.

Self Cite

View full text Add to dashboard Cite

Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications, such as stem cell therapy, tissue engineering, and assisted reproduction. Recent times have witnessed an increase in the clinical demand of these applications, requiring urgent improvements in cell cryopreservation. However, cryopreservation technology suffers from the issues of low cryopreservation efficiency and cryoprotectant (CPA) toxicity. Application of advanced biotechnology tools can significantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs, thus promoting the development of cryopreservation. Herein, based on the different cryopreservation mechanisms available, we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation, including trehalose delivery, hydrogel-based cell encapsulation technique, droplet-based cell printing, and nanowarming, and also discuss the associated challenges and perspectives for future development.

show abstract

Exploring the Potential of Biocompatible Osmoprotectants as Highly Efficient Cryoprotectants

Cited by 69 publications

References 59 publications

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture

Metabolic Pathway Profiling in Intracellular and Extracellular Environments of Streptococcus thermophilus During pH-Controlled Batch Fermentations

Advanced Biotechnology for Cell Cryopreservation

Contact Info

Product

Resources

About