Exploring the Potential of Venom from Nasonia vitripennis as Therapeutic Agent with High-Throughput Screening Tools

Danneels, Ellen L.; Formesyn, Ellen M.; Graaf, Dirk C. de

doi:10.3390/toxins7062051

Cited by 11 publications

(9 citation statements)

References 59 publications

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“…Recent examples of the use of high-throughput assay screening on venoms include the analysis of the venom from the wasp Nasonia vitripennis , which has indicated that it might have therapeutic potential. The use of reporter arrays showed the venom altered the expression of nuclear factor κβ (NF-κB) signalling pathway genes that have a role in inflammatory diseases and cancer [ 69 ]. Furthermore, a combined cytotoxicity screening and venom profiling approach on snake venom revealed the presence of activity against a human lung carcinoma cell line, and identification of a range of proteins including phospholipases and serine proteases [ 70 ].…”

Section: Venomicsmentioning

confidence: 99%

Venomics: A Mini-Review

Wilson

Daly

2018

High-Throughput

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Venomics is the integration of proteomic, genomic and transcriptomic approaches to study venoms. Advances in these approaches have enabled increasingly more comprehensive analyses of venoms to be carried out, overcoming to some extent the limitations imposed by the complexity of the venoms and the small quantities that are often available. Advances in bioinformatics and high-throughput functional assay screening approaches have also had a significant impact on venomics. A combination of all these techniques is critical for enhancing our knowledge on the complexity of venoms and their potential therapeutic and agricultural applications. Here we highlight recent advances in these fields and their impact on venom analyses.

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Section: Venomicsmentioning

confidence: 99%

Venomics: A Mini-Review

Wilson

Daly

2018

High-Throughput

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“…In this study, we examined the diversity of P. vindemmiae venom compositions based on both transcriptome-sequencing and proteome analysis, and compared it to venoms of three parasitoid families. Given the amazing toolkit available in its host drosophilids, we propose that future studies combining the power of Drosophila as a model system with its ectoparasitoid P. vindemmiae have great potentials for advancing our understanding of the functions and evolution of venom proteins, and assessing their pharmacological possibilities (Danneels et al, 2010(Danneels et al, , 2015Moreau and Asgari, 2015;Huerta-Rey et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Identification and Comparative Analysis of Venom Proteins in a Pupal Ectoparasitoid, Pachycrepoideus vindemmiae

Yang

Liu

et al. 2020

Front. Physiol.

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Parasitoid wasps inject venom containing complex bioactive compounds to regulate the immune response and development of host arthropods and sometime paralyze host arthropods. Although extensive studies have been conducted on the identification of venom proteins in larval parasitoids, relatively few studies have examined the pupal parasitoids. In our current study, a combination of transcriptomic and proteomic methods was used to identify 64 putative venom proteins from Pachycrepoideus vindemmiae, an ectoparasitoid of Drosophila. Expression analysis revealed that 20 tested venom proteins have 419-fold higher mean expression in the venom apparatus than in other wasp tissues, indicating their specialization to venom. Comparisons of venom proteins from P. vindemmiae and other five species spanning three parasitoid families detected a core set of "ancient" orthologs in Pteromalidae. Thirty-five venom proteins of P. vindemmiae were assigned to the orthologous groups by reciprocal best matches with venoms of other pteromalids, while the remaining 29 were not. Of the 35 categories, twenty-seven have orthologous relationships with Nasonia vitripennis venom proteins and 25 with venoms of Pteromalus puparum. More distant relationships detected that five and two venom proteins of P. vindemmiae are orthologous with venoms of two Figitidae parasitoids and a Braconidae representative, respectively. Moreover, twenty-two venoms unique to P. vindemmiae were also detected, indicating considerable interspecific variation of venom proteins in parasitoids. Phylogenetic reconstruction based on a set of single-copy genes clustered P. vindemmiae with P. puparum, N. vitripennis, and other members of the family Pteromalidae. These findings provide strong evidence that P. vindemmiae venom proteins are well positioned for future functional and evolutionary studies.

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“…Parasitoid wasps are the largest group of venomous animals, with somewhere between 100 000 and 500 000 species, but have been relatively understudied in venom research (Godfray, ; Quicke, ). These venoms are potentially a treasure trove of resources and potential targets for drug therapy for different diseases (Piek, ; Danneels et al ., ; Mrinalini and Werren ); however, a first step of this process is to understand the evolution and function of these venoms in their natural systems.…”

Section: Resultsmentioning

confidence: 99%

“…More recent studies using genome‐wide approaches have also found that envenomated fly hosts remain alive and transcriptionally active for over 5 days, and exhibit transcriptional changes that correspond to metabolic, immune response and developmental phenotypes (Danneels et al ., ; Martinson et al ., ; Siebert et al ., ). Yet most of the previous work has focused on whole venom effects in N. vitripennis , whereas relatively few studies have focused on specific venom proteins (However see Danneels et al ., ; Siebert et al ., ; Martinson et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Evaluating the evolution and function of the dynamic Venom Y protein in ectoparasitoid wasps

Martinson

Siebert

et al. 2019

Insect Molecular Biology

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Venom of the parasitoid wasp Nasonia vitripennis changes the metabolism and gene expression in its fly host Sarcophaga bullata to induce developmental arrest, suppression of the immune response and various other venom effects. Yet, the venom of ectoparasitoid wasps has not been fully characterized. A major component of N. vitripennis venom is an uncharacterized, high‐expressing protein referred to as Venom Y. Here we describe the evolutionary history and possible functions of this venom protein. We found that Venom Y is a relatively young gene that has duplicated to form two distinct paralogue groups. A copy of Venom Y has been recruited as a venom protein in at least five wasp species. Functional analysis found that Venom Y affects detoxification and immunity genes in envenomated fly hosts. Many of these genes are fat‐body specific, suggesting that Venom Y may have a targeted effect on fat body tissue. We also show that Venom Y may mitigate negative effects of other venom proteins. Finally, protein sequencing indicates that Venom Y is post‐translationally modified. This study contributes to elucidating parasitoid venom by using RNA interference knockdown to investigate venom protein function in the context of the whole venom cocktail.

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Exploring the Potential of Venom from Nasonia vitripennis as Therapeutic Agent with High-Throughput Screening Tools

Cited by 11 publications

References 59 publications

Venomics: A Mini-Review

Venomics: A Mini-Review

Identification and Comparative Analysis of Venom Proteins in a Pupal Ectoparasitoid, Pachycrepoideus vindemmiae

Evaluating the evolution and function of the dynamic Venom Y protein in ectoparasitoid wasps

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