Exploring the Regulation of tRNA Distribution on the Genomic Scale

Dittmar, Kimberly; Mobley, Evelyn M.; Radek, Agnes; Pan, Tao

doi:10.1016/j.jmb.2004.01.024

Cited by 89 publications

(108 citation statements)

References 33 publications

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“…In bacterial cells, tRNA represents up to 20 % of the total RNA (Dittmar et al, 2004). Although the basic features of tRNA were discovered almost 50 years ago (Hoagland et al, 1958), unexpected functions of this RNA were later demonstrated, including those connected with DNA replication.…”

Section: Discussionmentioning

confidence: 99%

tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I

Wang

Yuan

et al. 2006

Microbiology

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Effects of tRNA Ala (UGC) and its derivative devoid of the 39-ACCA motif [tRNA Ala (UGC)DACCA] on the cleavage of the ColE1-like plasmid-derived RNA I were analysed in vivo and in vitro. In an aminoacid-starved relA mutant, in which uncharged tRNAs occur in large amounts, three products of specific cleavage of RNA I were observed, in contrast to an otherwise isogenic relA + host. Overexpression of tRNA Ala (UGC), which under such conditions occurs in Escherichia coli mostly in an uncharged form, induced RNA I cleavage and resulted in an increase in ColE1-like plasmid DNA copy number. Such effects were not observed during overexpression of the 39-ACCA-truncated tRNA Ala (UGC). Moreover, tRNA Ala (UGC), but not tRNA Ala (UGC)DACCA, caused RNA I cleavage in vitro in the presence of MgCl 2 . These results strongly suggest that tRNA-dependent RNA I cleavage occurs in ColE1-like plasmid-bearing E. coli, and demonstrate that tRNA Ala (UGC) participates in specific degradation of RNA I in vivo and in vitro. This reaction is dependent on the presence of the 39-ACCA motif of tRNA Ala (UGC).

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Section: Discussionmentioning

confidence: 99%

tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I

Wang

Yuan

et al. 2006

Microbiology

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“…Pan and co-workers were the first to apply this technology for tRNA quantification. They designed microarray chips to quantify tRNA from different species, such as B. subtilis, E. coli, and H. sapiens (Dittmar et al 2004(Dittmar et al , 2005(Dittmar et al , 2006. In this technique, tRNA are labeled taking advantage of their common 3′ feature.…”

Section: The State Of the Trna Population In The Cellmentioning

confidence: 99%

Protein folding and tRNA biology

2017

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Polypeptides can fold into tertiary structures while they are synthesized by the ribosome. In addition to the amino acid sequence, protein folding is determined by several factors within the cell. Among others, the folding pathway of a nascent polypeptide can be affected by transient interactions with other proteins, ligands, or the ribosome, as well as by the translocation through membrane pores. Particularly, the translation machinery and the population of tRNA under different physiological or adaptive responses can dramatically affect protein folding. This review summarizes the scientific evidence describing the role of translation kinetics and tRNA populations on protein folding and addresses current efforts to better understand tRNA biology. It is organized into three main parts, which are focused on: (i) protein folding in the cellular context; (ii) tRNA biology and the complexity of the tRNA population; and (iii) available methods and technical challenges in the characterization of tRNA pools. In this manner, this work illustrates the ways by which functional properties of proteins may be modulated by cellular tRNA populations.

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“…The tRNA microarray method, including reproducibility and result validation by Northern blot, has been extensively described in previously published papers (Dittmar et al 2004(Dittmar et al , 2006Pavon-Eternod et al 2009Zhou et al 2009). …”

Section: Transfer Rna Microarraysmentioning

confidence: 99%

Overexpression of initiator methionine tRNA leads to global reprogramming of tRNA expression and increased proliferation in human epithelial cells

Pavon-Eternod

Gomes

Rosner

et al. 2013

RNA

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Transfer RNAs (tRNAs) are typically considered housekeeping products with little regulatory function. However, several studies over the past 10 years have linked tRNA misregulation to cancer. We have previously reported that tRNA levels are significantly elevated in breast cancer and multiple myeloma cells. To further investigate the cellular and physiological effects of tRNA overexpression, we overexpressed tRNA i Met in two human breast epithelial cell lines. We then determined tRNA abundance changes and performed phenotypic characterization. Overexpression of tRNA iMet significantly altered the global tRNA expression profile and resulted in increased cell metabolic activity and cell proliferation. Our results extend the relevance of tRNA overexpression in human cells and underscore the complexity of cellular regulation of tRNA expression.

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Exploring the Regulation of tRNA Distribution on the Genomic Scale

Cited by 89 publications

References 33 publications

tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I

tRNA-dependent cleavage of the ColE1 plasmid-encoded RNA I

Protein folding and tRNA biology

Overexpression of initiator methionine tRNA leads to global reprogramming of tRNA expression and increased proliferation in human epithelial cells

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