Exploring the role of Azadirachta indica (neem) and its active compounds in the regulation of biological pathways: an update on molecular approach

Sarkar, Subendu; Singh, Rajender Pal; Bhattacharya, Gourav

doi:10.1007/s13205-021-02745-4

Cited by 67 publications

(42 citation statements)

References 85 publications

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“…In addition, ImmuPlus ® is composed of the following plant parts: Tinospora cordifolia , Ocimum sanctum , Whitania somnifera , Andrographis paniculata, and Azadirachta indica . T. cordifolia contains 20.4% tannins and 2.19% flavonoids for antioxidant activity [ 59 ]; O. sanctum contains essential oils (24.15% camphor, 23.67% eugenol, and 13.47% eucalyptol) with antimicrobial properties [ 7 ]; W. somnifera contains flavonoids, tannins, and alkaloids with antimicrobial and antifungal properties [ 60 ]; A. paniculata contains 8.06% flavonoids and 10.22% essential oils with immunomodulatory and anti-inflammatory properties [ 8 , 61 ]; A. indica contains tannins, flavonoids, and essential oils with anti-inflammatory and antioxidant activity [ 62 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

et al. 2022

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The objective of this study was to evaluate the effects of dietary supplementation of a polyherbal additive (PA) containing hydrolyzable tannins, flavonoids, and essential oils on productive performance, dietary energetics, carcass and meat characteristics, and blood metabolites of lambs in their finishing phase. Twenty-eight Pelibuey × Katahdin lambs (20.52 ± 0.88 kg body weight (BW)) were housed in individual pens and assigned to four treatments (n = 7) with different doses of PA: 0 (CON), 1 (PA1), 2 (PA2), and 3 (PA3) g of PA kg−1 of DM for 56 days. Compared to the CON, lambs in PA1 treatment had higher average daily gain (p = 0.03), higher dietary energy utilization (p = 0.01), greater backfat thickness (p = 0.02), greater Longissimus dorsi muscle area (p = 0.01), and better feed conversion ratio (p = 0.02). PA supplementation did not affect (p > 0.05) dry matter intake, carcass yield, biometric measures, and meat chemical composition. All hematological and most of the blood biochemical parameters were similar in lambs of all treatments (p > 0.05). However, compared to the CON, lambs assigned to the PA3 treatment had lower serum urea concentration (p = 0.05) and higher serum albumin concentration (p = 0.03). In conclusion, low doses of PA could be used as a growth promoter in finishing lambs without affecting dry matter intake, carcass yield, meat chemical composition, and health status of the lambs. However, more in vivo research is needed to better understand the impact of bioactive compounds from PA used on productivity, metabolism, and health status of finishing lambs.

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Section: Methodsmentioning

confidence: 99%

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

et al. 2022

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“…A. indica (Neem) belongs to Mahogany family and has been used as herbal medicine, particularly in Africa and south of Asia [ 57 ]. The Neem extracts have exhibited immunomodulatory, anti-hyperglycemic, anti-carcinogenic, anti-parasitic, antiviral, insecticidal, and antioxidant properties [ 16 , 17 , 19 , 58 – 60 ]. Blum et al [ 19 ] evaluated the anti- Helicobacter pylori effects of the NeO and suggested that this oil has promising bactericidal effects and would be considered for further treatment strategies against this bacterium.…”

Section: Discussionmentioning

confidence: 99%

Formulation of Neem oil-loaded solid lipid nanoparticles and evaluation of its anti-Toxoplasma activity

Nemati

Rahimi

Hesari

et al. 2022

BMC Complement Med Ther

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Background Toxoplasmosis is caused by an intracellular zoonotic protozoan, Toxoplasma gondii, which could be lethal in immunocompromised patients. This study aimed to synthesize Neem oil-loaded solid lipid nanoparticles (NeO-SLNs) and to evaluate the anti-Toxoplasma activity of this component. Methods The NeO-SLNs were constructed using double emulsification method, and their shape and size distribution were evaluated using transmission electron microscope (TEM) and dynamic light scattering (DLS), respectively. An MTT assay was employed to evaluate the cell toxicity of the component. The anti-Toxoplasma activity of NeO-SLNs was investigated using vital (trypan-blue) staining. Anti-intracellular Toxoplasma activity of NeO-SLNs was evaluated in T. gondii-infected Vero cells. Results The TEM analysis represented round shape NeO-SLNs with clear and stable margins. DLS analysis showed a mean particle size 337.6 nm for SLNs, and most of nanoparticles were in range 30 to 120 nm. The cell toxicity of NeO-SLNs was directly correlated with the concentration of the component (P-value = 0.0013). The concentration of NeO-SLNs, which was toxic for at least 50% of alive T. gondii (cytotoxic concentration (CC50)), was > 10 mg/mL. The ability of NeO-SLNs to kill Toxoplasma was concentration-dependent (P-value < 0.0001), and all concentrations killed at least 70% of alive tachyzoites. Furthermore, the viability of T. gondii- infected Vero cells was inversely correlated with NeO-SLNs concentrations (P-value = 0.0317), and in the concentration 100 μg/mL at least 75% of T. gondii- infected Vero cells remained alive. Conclusions Overall, our findings demonstrated that the NeO-SLNs was able to kill T. gondii tachyzoites in concentration 100 μg/mL with a cell toxicity lower than 20%. Such results suggest that employing SLNs as carrier for NeO can effectively kill T. gondii tachyzoites with acceptable cell toxicity. Our findings also showed that SLNs capsulation of the NeO can lead to prolonged release of the extract, suggesting that NeO-SLNs could be also employed to clear cyst stages, which should be further investigated in animal models.

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“…6 Also, azadirachtin, the most active compound of Neem, is toxic for insects, while the bitterness of Neem is mainly due to the accumulation of limonoids. 7 Several chemical components of different parts and extracts of Neem have also been reported to possess remarkable anti-inflammatory, anti-arthritics, hypoglycemic, antipyretic, spermicidal, antifungal, antibacterial, antimalarial, immunomodulatory, antitumor, diuretic, [8][9][10] anticancer, and antibiofilm. 11,12 Azadirachtin, which is extracted from neem seeds, has several adverse effects on insect pests, including pest repellency, feeding inhibition, decreased oviposition, sterilization, behavioral changes, and increased mortality.…”

Section: Introductionmentioning

confidence: 99%

Bibliometric Analysis of 100 Top-Cited Articles on Neem Indexed in the Web of Science

2022

TJNPR

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Recently, there has been a surge in interest in evaluating research accomplishments based on publications using bibliometric tools. This study aims to investigate the top 100 most cited articles in Neem using bibliometric analysis while also providing readers with a practical guide for determining the most influential publications over the past decades. VOSviewer 1.6.6, the "Bibliometrix app" in R. studio cloud, and SPSS were used to analyze the data. The top 100 highly-cited articles in Neem, which included 91 articles and nine reviews, were published between 1971 and 2017. The articles received a total citation score of 14,186 (citations ranging from 65 to 1,531), with an average total citation score of 141.9 and a total citation score per article of 10.07. 398 authors from 22 countries contributed a total of 100 publications, with Bhattacharyya KG being the most productive and influential. Researchers from India published about 45 percent of the articles and had the highest number of citations (8637). The Banaras Hindu University in India was discovered to be the top-ranked institution, with six publications and 1,491 citations. The top 100 literature was published in 72 journals, with the Journal of Ethnopharmacology publishing nine papers. The findings of this study provided extensive information and research direction for future research in this domain, including the most influential journals, countries, authors, institutions, and articles and mapping co-authorship and reported keywords.

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Exploring the role of Azadirachta indica (neem) and its active compounds in the regulation of biological pathways: an update on molecular approach

Cited by 67 publications

References 85 publications

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

Effects of a Polyherbal Dietary Additive on Performance, Dietary Energetics, Carcass Traits, and Blood Metabolites of Finishing Lambs

Formulation of Neem oil-loaded solid lipid nanoparticles and evaluation of its anti-Toxoplasma activity

Bibliometric Analysis of 100 Top-Cited Articles on Neem Indexed in the Web of Science

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