2018
DOI: 10.1002/btpr.2722
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Exploring the separation power of mixed‐modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates

Abstract: Osteopontin (OPN) is a structural protein with potential value in therapeutic and diagnostic applications. Low titer, acidic isoelectric point, and the lack of well‐defined secondary and tertiary structure were some of the challenges that complicated purification development of OPN from recombinant Escherichia coli lysates. Reported processes for OPN recovery from recombinant sources use nonorthogonal unit operations and often suffer from low yield. In this work, we expanded the search for an optimal OPN purif… Show more

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Cited by 2 publications
(1 citation statement)
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“…In a recent study, using 96well-plates containing nickel-functionalized membranes, rapid screening of parameters for membrane protein purification was successfully performed (Feroz et al, 2019). Mixed-mode resins (ionic and hydrophobic interactions) were used in a plate-based HT screening platform for the selection of process parameters to achieve high purity and high overall yield of osteopontin (Guo et al, 2019). A novel microfluidics-based methodology to carry out speedy and multiplexed screening of several MM ligands relative to their potential to bind different target molecules was studied using an artificial mixture (containing IgG and BSA, labeled with different thiol-reactive neutral fluorescent dyes).…”
Section: High-throughput Technologiesmentioning
confidence: 99%
“…In a recent study, using 96well-plates containing nickel-functionalized membranes, rapid screening of parameters for membrane protein purification was successfully performed (Feroz et al, 2019). Mixed-mode resins (ionic and hydrophobic interactions) were used in a plate-based HT screening platform for the selection of process parameters to achieve high purity and high overall yield of osteopontin (Guo et al, 2019). A novel microfluidics-based methodology to carry out speedy and multiplexed screening of several MM ligands relative to their potential to bind different target molecules was studied using an artificial mixture (containing IgG and BSA, labeled with different thiol-reactive neutral fluorescent dyes).…”
Section: High-throughput Technologiesmentioning
confidence: 99%