Access to treated water has increased in urban areas of the developing world in recent decades: However, many regions still lack proper sanitation or safe drinking water. Among the existing studies on low-cost water treatment methods to solve this problem, the application of cacti species is still a recent one. Considering this, the present study aimed to evaluate the best cactus species (Opuntia ficus-indica, Cereus jamacaru and Cereus jamacaru monstrosus) on the simultaneous removal of pathogens, improvement in physical-chemical conditions, without letting cytotoxic residues on water. Tests were carried out using river water and small pieces of cladodes at dosages of 15, 25, 50 and 75 (g L −1) in agitation periods of 2 min at 60 rpm and settling periods of 30, 120 and 360 min. O. ficus-indica performed better than C. jamacaru in removing E. coli, turbidity, color, iron and nitrate, and a dosage of 25 g L −1 of cactus was found to be the most efficient for water treatment. This dosage resulted in a maximum non-cytotoxic exposition time of 30 min for O. ficus-indica and of 120 min for C. jamacaru. Considering how efficient these cacti have shown to be in removing pollutants, and how widespread and easy to cultivate they are, they could be considered as a safe, low-cost alternative to other coagulation-flocculation products in water treatment processes.