Explosives detection in the marine environment using UUV-modified immunosensor

Charles, Paul T.; Adams, André A.; Deschamps, Jeffrey R.; Veitch, Scott P.; Hanson, Alfred K.; Kusterbeck, Anne W.

doi:10.1117/12.883243

Cited by 3 publications

(1 citation statement)

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“…In the past decade, explosives detection technologies have advanced significantly as a result of novel scientific contributions in polymer synthesis [10,11] and microfluidics engineering and design [12,13]. Electrochemical sensors [14][15][16], immunosensors [17][18][19][20][21], novel fluorescent polymers [22], molecularly imprinted polymers (MIP) coupled to surface enhanced Raman spectroscopy (SERS) devices [23], lateral flow assays [24] and nanosensors [25] all have contributed significantly in advancing the field. However, the desired goal for end users who wish to detect explosives in environmentally relevant media is an analytical system that consists of the following: (1) sensitivity with sub-parts-per-billion (ppb) detection levels, (2) specificity for the explosive molecules of interest, (3) no required pre-concentration steps, (4) on-site chemical analysis capability, and (5) provides quantitative information from aqueous samples.…”

Section: Introductionmentioning

confidence: 99%

Multi-channeled single chain variable fragment (scFv) based microfluidic device for explosives detection

Charles

Davis

Adams

et al. 2015

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The development of explosives detection technologies has increased significantly over the years as environmental and national security agencies implement tighter pollution control measures and methods for improving homeland security. 2, 4, 6-Trinitrotoluene (TNT), known primarily as a component in munitions, has been targeted for both its toxicity and carcinogenic properties that if present at high concentrations can be a detriment to both humans, marine and plant ecosystems. Enabling end users with environmental detection and monitoring systems capable of providing real-time, qualitative and quantitative chemical analysis of these toxic compounds would be extremely beneficial. Reported herein is the development of a multi-channeled microfluidic device immobilized with single chain fragment variable (scFv) recombinant proteins specific for the explosive, TNT. Fluorescence displacement immunoassays performed under constant flow demonstrated trace level sensitivity and specificity for TNT. The utility of three multi-channeled devices immobilized with either (1) scFv recombinant protein, (2) biotinylated-scFv (bt-scFv) and (3) monoclonal anti-TNT (whole IgG molecule) were investigated and compared. Fluorescence dose response curves, crossreactivity measurements and limits of detection (LOD) for TNT were determined. Fluorescence displacement immunoassays for TNT in natural seawater demonstrated detection limits at sub-parts-per-billion levels (0.5 ppb) utilizing the microfluidic device with immobilized bt-scFv.

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Section: Introductionmentioning

confidence: 99%

Multi-channeled single chain variable fragment (scFv) based microfluidic device for explosives detection

Charles

Davis

Adams

et al. 2015

Talanta

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Advances in explosives analysis—part I: animal, chemical, ion, and mechanical methods

et al. 2015

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The number and capability of explosives detection and analysis methods have increased substantially since the publication of the Analytical and Bioanalytical Chemistry special issue devoted to Explosives Analysis (Moore and Goodpaster, Anal Bioanal Chem 395(2):245-246, 2009). Here we review and critically evaluate the latest (the past five years) important advances in explosives detection, with details of the improvements over previous methods, and suggest possible avenues towards further advances in, e.g., stand-off distance, detection limit, selectivity, and penetration through camouflage or packaging. The review consists of two parts. This part, Part I, reviews methods based on animals, chemicals (including colorimetry, molecularly imprinted polymers, electrochemistry, and immunochemistry), ions (both ion-mobility spectrometry and mass spectrometry), and mechanical devices. Part II will review methods based on photons, from very energetic photons including X-rays and gamma rays down to the terahertz range, and neutrons.

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