Exposing culprit organic pollutants: A review

Keane, Angela; Phoenix, Pauline; Ghoshal, Subhasis; Lau, Peter C. K.

doi:10.1016/s0167-7012(01)00382-7

Cited by 82 publications

(32 citation statements)

References 93 publications

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“…9,10 In this manner, bacterial bioreporters have been developed for heavy metals 11 and several organic compounds. 9,12 In heavy metal bioreporters, the sensor element usually consists of a specific promoter fused to a reporter gene, and a gene encoding for a transcription factor that in response to certain metal ions activates the promoter. Binding of a metal ion to the transcription factor induces the expression of the reporter gene, thus converting metal sensing into a detectable output signal.…”

Section: Introductionmentioning

confidence: 99%

Improving the sensitivity of bacterial bioreporters for heavy metals

2010

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Section: Introductionmentioning

confidence: 99%

Improving the sensitivity of bacterial bioreporters for heavy metals

2010

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“…The wide diversity of whole-cell biosensors has been extensively reviewed (Daunert et al, 2000;Keane et al, 2002). While other engineered bioluminescent bacteria have been developed for the detection of specific organic compounds, they are all based on fusions with promotors from specific catabolic pathways.…”

Section: Description Of Biosensormentioning

confidence: 99%

Use of a whole‐cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants

Keane

Lau

Ghoshal

2007

Biotech & Bioengineering

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ABSTRACT:The whole-cell bioluminescent biosensor Pseudomonas putida F1G4 (PpF1G4), which contains a chromosomally-based sep-lux transcriptional fusion, was used as a tool for direct measurement of the bioavailability of hydrophobic organic compounds (HOCs) partitioned into surfactant micelles. The increased bioluminescent response of PpF1G4 in micellar solutions (up to 10 times the critical micellar concentration) of Triton X-100 and Brij 35 indicated higher intracellular concentrations of the test compounds, toluene, naphthalene, and phenanthrene, compared to control systems with no surfactants present. In contrast, Brij 30 caused a decrease in the bioluminescent response to the test compounds in single-solute systems, without adversely affecting cell growth. The decrease in bioluminescent response in the presence of Brij 30 did not occur in the presence of multiple HOCs extracted into the surfactant solutions from crude oil and creosote. The effect of the micellar solutions on the toluene biodegradation rate was consistent with the bioluminescent response in single-solute systems. None of the surfactants were toxic to PpF1G4 at the doses employed in this study, and PpF1G4 did not produce a bioluminescent response to the surfactants nor utilize them as growth substrates. TEM images suggest that the surfactants did not rupture the cell membranes. The results demonstrate that for Pseudomonas putida F1, nonionic surfactants such as Triton X-100 and Brij 35, at doses between 2 and 10 CMC, may increase the bioavailability and direct uptake of micellar phase HOCs that are common pollutants at contaminated sites. Biotechnol. Bioeng. 2008;99: 86-98.

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“…This genetic information, located on a plasmid vector, is then inserted into a bacterial strain, which may be integrated into the chromosome or remain in plas-mid form. The engineered genetic fusion is then replicated along with the cell's normal DNA [163]. As reported above, in the case of bioluminescence sensors, the recombinant bacteria employed contains gene fusions between the regulatory region of the mer operon (merR) and bacterial luminescence genes, which respond to contaminants.…”

Section: Bioengineeringmentioning

confidence: 99%

Biosensors for environmental applications: Future development trends

Rodríguez-Mozaz

Marco

Alda

et al. 2004

Pure and Applied Chemistry

199

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Biosensors can be excellent analytical tools for monitoring programs working to implement legislation. In this article, biosensors for environmental analysis and monitoring are extensively reviewed. Examples of biosensors for the most important families of environmental pollutants, including some commercial devices, are presented. Finally, future trends in biosensor development are discussed. In this context, bioelectronics, nanotechnology, miniaturization, and especially biotechnology seem to be growing areas that will have a marked influence on the development of new biosensing strategies in the next future.

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Exposing culprit organic pollutants: A review

Cited by 82 publications

References 93 publications

Improving the sensitivity of bacterial bioreporters for heavy metals

Improving the sensitivity of bacterial bioreporters for heavy metals

Use of a whole‐cell biosensor to assess the bioavailability enhancement of aromatic hydrocarbon compounds by nonionic surfactants

Biosensors for environmental applications: Future development trends

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