Exposure of Carcinus maenas to waterborne fluoranthene: Accumulation and multibiomarker responses

Rodrigues, Aurélie Pinto; Lehtonen, Kari K.; Guilhermino, Lúcia; Guimarães, Laura

doi:10.1016/j.scitotenv.2012.10.077

Cited by 50 publications

(18 citation statements)

References 72 publications

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“…Respiration rates, which in FSW were similar to those reported for M. leidyi previously (e.g. Anninsky et al 2005, Thuesen et al 2005, were not significantly elevated in any test solution, as might be expected with an increase in aerobic metabolic demand from upregulation of detoxification pathways (Hatlen et al 2009, Rodrigues et al 2013 or from attempted avoidance of the toxicants (Seuront 2010). As respiration rate experiments employed single endpoint measurements and were terminated after 24 h, when ctenophores were then assayed for bioluminescence, it is not known whether alterations in respiration rate and/or GST activity (in oil treatments and/or in 23°C exposures) occurred but only lasted a short time or if they would occur with longer exposure durations.…”

Section: Discussionsupporting

confidence: 87%

Lethal and sublethal effects of oil, chemical dispersant, and dispersed oil on the ctenophore Mnemiopsis leidyi

Peiffer¹,

Cohen²

2015

Aquat. Biol.

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We established the lethal levels for water-accommodated fractions of Corexit ® 9500A chemical dispersant, crude oil (WAF), and dispersed crude oil (CEWAF) for the ctenophore Mnemiopsis leidyi at both 15 and 23°C. This gelatinous zooplankter was sensitive to dispersant at both temperatures, as well as to oil solutions, with some increase in toxicity of CEWAF as compared to WAF. Subsequent sublethal assays for routine respiration rate, bioluminescence, and glutathiones-transferase (GST) activity were conducted on individuals surviving 24 h exposures to test solutions at both 15 and 23°C. GST activity increased significantly in 2.5 and 5 mg l −1 dispersant solutions at 15°C, suggesting a metabolic detoxification response to the dispersant-containing solutions, but no effect of any solution type on routine respiration rate was observed. Light emission through mechanically stimulated bioluminescence and photocyte lysis decreased with exposure to crude oil WAF and CEWAF at both temperatures and to dispersant exposure at 23°C.Collectively, these results demonstrate that M. leidyi exhibits both lethal and sublethal effects from acute crude oil exposure, with an elevation of some sublethal responses upon addition of chemical dispersant. Sublethal effects of oil and dispersants in pelagic species, most notably impairment of luminescence, should be considered when evaluating oil spill response strategies.

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Section: Discussionsupporting

confidence: 87%

Lethal and sublethal effects of oil, chemical dispersant, and dispersed oil on the ctenophore Mnemiopsis leidyi

Peiffer¹,

Cohen²

2015

Aquat. Biol.

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“…In this study, the marked increase of GST activity was observed in the fi sh liver exposed to Fl and Fluo alone. The increased GST activity by Fl or Fluo was also recorded in the same species [4,33] and different species, such as crab (Carcinus maenas) [34]. These results suggest the involvement of GST in the detoxifi cation of these two PAHs and the occurrence of oxidative stress response in aquatic organisms to Fl and Fluo exposure.…”

Section: Discussion Of Resultssupporting

confidence: 57%

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Lu¹,

Ding

2018

Pol. J. Environ. Stud.

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To evaluate potential interactive effects of metals and polycyclic aromatic hydrocarbons (PAHs) on biomarker responses, groups of the freshwater fi sh crucian carp (Carassius auratus) were exposed to single and binary combinations of copper (Cu) (0.01-0.16 mg L-1) with fl uorene (Fl) or fl uoranthene (Fluo) (2-10 mg kg -1 ) for 96 h. Dose-dependent increases in the activities of phases I and II metabolic enzymes [7-ethoxyresorufi n O-deethylase (EROD) and glutathione-S-transferase (GST), respectively] were observed in fi sh liver exposed to Fl and Fluo, but these enzyme activities did not differ signifi cantly from the controls when co-treated with higher concentrations of Cu, suggesting an inhibiting interaction on the metabolic enzymes. Although Cu did not alter catalase (CAT) activity, CAT activity was decreased in fi sh liver exposed to the two PAHs alone or in combination with Cu. Although metallothionein (MT) content in gills was signifi cantly increased following exposure to Cu alone or in combination with Fl and Fluo, the induction folds of MT decreased under co-exposure. Co-exposures to these chemicals invoked complex biomarker responses in fi sh liver and gills. These results highlight the need for careful consideration of the interactive effects of multiple environmental stressors on fi sh.

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“…In the micro-PS þ FLU exposed mussels, an efficient neutralization of the ROS is suggested by the activation of anti-oxidant and glutathione related markers and low LPO levels. Low LPO levels suggesting a suitable neutralization of ROS was previously hypothesized in the crustacean Carcinus maenas (Rodrigues et al, 2013), and the molluscs Perna viridis (Cheung et al, 2001) and Chlamys farreri (Xiu et al, 2014) exposed to FLU.…”

Section: Modulation Of Anti-oxidant Defences and Oxidative Damagesmentioning

confidence: 56%

“…No significant effects were observed on biochemical composition, concentration and viability of algae (data not shown). The final concentrations of acetone (0.003%) and Tween-20 (0.00001%) in the experimental tanks were much lower than the toxic levels reported for marine invertebrates (Rodrigues et al, 2013;Ostroumov, 2003;Sussarellu et al, 2016).…”

Section: Mussel Exposurementioning

confidence: 62%

Exposure of marine mussels Mytilus spp. to polystyrene microplastics: Toxicity and influence on fluoranthene bioaccumulation

Paul-Pont

Lacroix

González-Fernández

et al. 2016

Environmental Pollution

574

224

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The effects of polystyrene microbeads (micro-PS; mix of 2 and 6 μm; final concentration: 32 μg L(-1)) alone or in combination with fluoranthene (30 μg L(-1)) on marine mussels Mytilus spp. were investigated after 7 days of exposure and 7 days of depuration under controlled laboratory conditions. Overall, fluoranthene was mostly associated to algae Chaetoceros muelleri (partition coefficient Log Kp = 4.8) used as a food source for mussels during the experiment. When micro-PS were added in the system, a fraction of FLU transferred from the algae to the microbeads as suggested by the higher partition coefficient of micro-PS (Log Kp = 6.6), which confirmed a high affinity of fluoranthene for polystyrene microparticles. However, this did not lead to a modification of fluoranthene bioaccumulation in exposed individuals, suggesting that micro-PS had a minor role in transferring fluoranthene to mussels tissues in comparison with waterborne and foodborne exposures. After depuration, a higher fluoranthene concentration was detected in mussels exposed to micro-PS and fluoranthene, as compared to mussels exposed to fluoranthene alone. This may be related to direct effect of micro-PS on detoxification mechanisms, as suggested by a down regulation of a P-glycoprotein involved in pollutant excretion, but other factors such as an impairment of the filtration activity or presence of remaining beads in the gut cannot be excluded. Micro-PS alone led to an increase in hemocyte mortality and triggered substantial modulation of cellular oxidative balance: increase in reactive oxygen species production in hemocytes and enhancement of anti-oxidant and glutathione-related enzymes in mussel tissues. Highest histopathological damages and levels of anti-oxidant markers were observed in mussels exposed to micro-PS together with fluoranthene. Overall these results suggest that under the experimental conditions of our study micro-PS led to direct toxic effects at tissue, cellular and molecular levels, and modulated fluoranthene kinetics and toxicity in marine mussels.

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Exposure of Carcinus maenas to waterborne fluoranthene: Accumulation and multibiomarker responses

Cited by 50 publications

References 72 publications

Lethal and sublethal effects of oil, chemical dispersant, and dispersed oil on the ctenophore Mnemiopsis leidyi

Lethal and sublethal effects of oil, chemical dispersant, and dispersed oil on the ctenophore Mnemiopsis leidyi

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Exposure of marine mussels Mytilus spp. to polystyrene microplastics: Toxicity and influence on fluoranthene bioaccumulation

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