Exposure to acute waterborne cadmium caused severe damage on lipid metabolism of freshwater fish, revealed by nuclear lipid droplet deposition in hepatocytes of rare minnow

Liu, X. H.; Pang, Xu; Jin, Li; Pu, Deyong; WANG, Zhi-Jian; Zhang, Yaoguang

doi:10.1016/j.aquatox.2023.106433

Cited by 15 publications

(2 citation statements)

References 76 publications

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“…Interestingly, a significant increase in lipid content and cytoplasmic vacuolizations was synchronously detected in all D. rerio samples exposed to Pb, thus suggesting that the appearance of vacuole structures could be related to lipid depositions. Our findings are, in general, in agreement with the data from the literature on rare minnows (Gobiocypris rarus) when they are subjected to acute waterborne cadmium exposure [65].…”

Section: Morphological Modificationssupporting

confidence: 92%

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Macirella

Curcio

Ahmed

et al. 2023

Fishes

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Lead (Pb) is a non-essential, highly toxic, and persistent element widely recognized as one of the most concerning pollutants. It is listed on the Priority List of Hazardous Substances. Widespread environmental contamination from Pb is a serious issue for human health and wildlife. In fish, Pb mainly accumulates in the liver, which is a key component for metal detoxification and excretion processes. In this study, we investigated, for the first time, the morphological and functional injuries induced in zebrafish (Danio rerio) liver by two very low and environmentally relevant concentrations of Pb (2.5 and 5 μg/L) after 48, 96, and 192 h of exposure. We observed significant histological alterations in all the exposed samples, and it was demonstrated that the extent of injuries increased with dose and exposure time. The most common modifications observed were congestion of blood vessels and sinusoids, cytoplasmic vacuolizations, parenchyma dyschromia, and macrophage proliferation. Pb administration also resulted in a significant increase in lipid content and the upregulation of key genes that are involved in metal detoxification (mtf1) and the defensive response against oxidative stress (sod1 and cat). We show that even very low doses of Pb can disrupt liver morphology and function.

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Section: Morphological Modificationssupporting

confidence: 92%

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Macirella

Curcio

Ahmed

et al. 2023

Fishes

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“…The identification of nuclear LDs in mammalian cells has been expanded using modern microscopic techniques, by our group 16 – 18 and others 19 – 23 . Nuclear LDs have been also found in other non-mammalian cells such as budding yeast 24 – 28 , plant cells 29 , C. elegans intestinal cells 30 , 31 , and fish hepatocytes treated with heavy metals 32 . Nuclear LDs can recruit and activate CDP-choline diacylglycerol phosphotransferase α (CCTα)—a rate-limiting enzyme of the de novo phosphatydilcholine (PC) synthesis pathway 33 —and diacylglycerol acyltransferase and be involved in PC or triglyceride synthesis in hepatoma cells and osteosarcoma cells 18 , 21 , 34 .…”

Section: Introductionmentioning

confidence: 87%

Distinct features of two lipid droplets types in cell nuclei from patients with liver diseases

Imai

Ohsaki

Cheng

et al. 2023

Sci Rep

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Lipid droplets (LDs) have been observed in the nuclei of hepatocytes; however, their significance in liver disease remains unresolved. Our purpose was to explore the pathophysiological features of intranuclear LDs in liver diseases. We included 80 patients who underwent liver biopsies; the specimens were dissected and fixed for electron microscopy analysis. Depending on the presence of adjacent cytoplasmic invagination of the nuclear membrane, LDs in the nuclei were classified into two types: nucleoplasmic LDs (nLDs) and cytoplasmic LD invagination with nucleoplasmic reticulum (cLDs in NR). nLDs were found in 69% liver samples and cLDs in NR were found in 32%; no correlation was observed between the frequencies of the two LD types. nLDs were frequently found in hepatocytes of patients with nonalcoholic steatohepatitis, whereas cLDs in NR were absent from the livers of such patients. Further, cLDs in NR were often found in hepatocytes of patients with lower plasma cholesterol level. This indicates that nLDs do not directly reflect cytoplasmic lipid accumulation and that formation of cLDs in NR is inversely correlated to the secretion of very low-density lipoproteins. Positive correlations were found between the frequencies of nLDs and endoplasmic reticulum (ER) luminal expansion, suggesting that nLDs are formed in the nucleus upon ER stress. This study unveiled the presence of two distinct nuclear LDs in various liver diseases.

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Role of UV radiation and oxidation on polyethylene micro- and nanoplastics: impacts on cadmium sorption, bioaccumulation, and toxicity in fish intestinal cells

Pinto,

Scott,

Hess

et al. 2024

Environ Sci Pollut Res

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This study investigated the role of ultraviolet (UV) radiation and oxidation in high-density polyethylene microplastics (2–15 μm) and nanoplastics (0.2–9.9 μm) (NMPs) on particle chemistry, morphology, and reactivity with cadmium (Cd). Additionally, toxicity of NMPs alone and with Cd was evaluated using RTgutGC cells, a model of the rainbow trout (Oncorhynchus mykiss) intestine. The role on NMPs on Cd bioaccumulation in RTgutGC cells was also evaluated. Dynamic light scattering indicated that after UV radiation NPs agglomerated size increased from 0.8 to 28 µm, and to 8 µm when Cd was added. Oxidized MPs agglomerated size increased from 11 and 7 to 46 and 27 µm in non-UV- and UV-aged oxidized MPs when adding Cd, respectively. Cd-coated particles exhibited generally significantly higher zeta potential than non-Cd-coated particles, while attenuated total reflectance–Fourier transform infrared spectroscopy showed that the functional chemistry of the particles was oxidized and modified after being exposed to UV radiation. Presence of NMPs resulted in a significant decrease in Cd bioaccumulation in RTgutGC cells (100.5–87.9 ng Cd/mg protein) compared to Cd alone (138.1 ng Cd/mg protein), although this was not quite significant for co-exposures with UV-aged NPs (105.7 ng Cd/mg protein). No toxicity was observed in RTgutGC cells exposed to NMPs alone for 24 h. Moreover, co-exposures with Cd indicated that NMPs reduce the toxicity of Cd. Altogether these results show that UV aging enhances NMP surface reactivity, increasing Cd absorption in solution, which resulted in a reduction in Cd bioavailability and toxicity.

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Exposure to acute waterborne cadmium caused severe damage on lipid metabolism of freshwater fish, revealed by nuclear lipid droplet deposition in hepatocytes of rare minnow

Cited by 15 publications

References 76 publications

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Morphological and Functional Alterations in Zebrafish (Danio rerio) Liver after Exposure to Two Ecologically Relevant Concentrations of Lead

Distinct features of two lipid droplets types in cell nuclei from patients with liver diseases

Role of UV radiation and oxidation on polyethylene micro- and nanoplastics: impacts on cadmium sorption, bioaccumulation, and toxicity in fish intestinal cells

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