Exposure to extremely low frequency electromagnetic fields alters the calcium dynamics of cultured entorhinal cortex neurons

Luo, Fenlan; Yang, Ning; He, Chao; Li, Hongli; Li, Chao; Chen, Fang; Xiong, Jiaxiang; Hu, Zhian; Zhang, Jun

doi:10.1016/j.envres.2014.09.023

Cited by 24 publications

(21 citation statements)

References 57 publications

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“…Increased intracellular calcium ion concentration leads to calmodulin‐dependent NOS activation and increased intracellular NO production [Pilla, ]. In addition, another study using the patch clamp technique showed that EMF exposure specifically influenced intracellular calcium ion dynamics and altered Ca 2+ signaling events [Luo et al, ]. Our results confirmed previous results reporting the effect of EMF on external and internal calcium ion flow.…”

Section: Discussionsupporting

confidence: 90%

Stable morphological–physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide

Haghighat

Abdolmaleki

Behmanesh

et al. 2017

Bioelectromagnetics

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As an external physical factor, electromagnetic field (EMF) may influence cellular processes and nitric oxide (NO) by serving as a secondary messenger molecule in intracellular signaling cascades. Effects of these factors were evaluated simultaneously on viability, morphology, and variation of calcium ion content, and neural protein marker expression in rat bone marrow mesenchymal stem cells (BMSCs). The mesenchymal stem cells were isolated from rat bone marrow and cultured. Deta-NO as a donor molecule of NO was added to cell culture medium after several passages. These cells were also exposed by retinoic acid (RA, a molecule inducing cell differentiation) and EMF (50 Hz and 20 mT). Despite the effect observed with low concentration of NO, the high concentration of NO in the presence of EMF decreased cell viability and changed cell morphology. EMF increased entry of calcium ion into the cell. Effect of RA on cell death and morphology changes also intensified in the presence of NO and EMF. BMSCs maintained their proliferative state and continued to remain as a stem cell in low concentration of NO. The decrease of cell viability, and increase in number and length of cell neurites and percentage of cells expressing Map2 marker can be a sign of progression for cell neuronal differentiation treated by high concentration of NO with EMF. Bioelectromagnetics. 38:592-601, 2017. © 2017 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 90%

Stable morphological–physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide

Haghighat

Abdolmaleki

Behmanesh

et al. 2017

Bioelectromagnetics

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“…In the present research, we examined primary cultured rat hippocampal neurons as a cellular model to understand the mechanisms of ELF-EMF-induced neuronal apoptosis. A decrease in cell viability and an increase in cell apoptosis were detected in hippocampal neurons exposed to ELF-EMF, which were consistent with previous study where apoptosis was found in hippocampal neurons exposed to ELF-EMF [24]. However, treatment with LSPCs (2.5, 5 and 10 mg/ml) in hippocampal neurons significantly increased the cell viability and reduced the total number of apoptotic cells, thereby providing a neuroprotective effect.…”

Section: Discussionsupporting

confidence: 93%

Neuroprotective effects of lotus seedpod procyanidins on extremely low frequency electromagnetic field-induced neurotoxicity in primary cultured hippocampal neurons

Yin

Duan

et al. 2016

Biomedicine & Pharmacotherapy

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“…The CAT activity was significantly enhanced by epicatechin and catechin treatment at the dose of 90 mg/kg ( p < .01) compared with the ELF‐EMF group in the brain (Table ). Many studies have reported the association between ELF‐EMF and the learning and memory (Luo et al, ). Oxidative stress appears to be responsible of free radicals in rat brain caused by ELF‐EMF (Falone et al, ).…”

Section: Resultsmentioning

confidence: 99%

Beneficial effect of catechin and epicatechin on cognitive impairment and oxidative stress induced by extremely low frequency electromagnetic field

2017

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This study aimed to explore the effects of catechin and epicatechin on the cognitive deficits and oxidative damage of mice at extremely low frequency electromagnetic field (ELF‐EMF) exposure (50 Hz, 200 µT, 30 days). The results showed 90 mg/kg catechin and epicatechin treatment could keep proper body weight compared with ELF‐EMF group. Water maze test indicated learning and memory abilities of ELF‐EMF group deteriorated significantly compared with control group, but ELF‐EMF + (catechin and epicatechin) 90 group improved learning and memory abilities compared with ELF‐EMF group. Malonaldehyde (MDA) and nitric oxide (NO) increased remarkably, while catalase (CAT) and superoxide dismutase (SOD) activities decreased significantly in ELF‐EMF group. Catechin and epicatechin (90 mg/kg) administration decreased MDA and NO levels and significantly increased CAT and SOD activities. The results suggested catechin and epicatechin can be beneficial for learning and memory impair and reducing oxidative stress under ELF‐EMF exposure through scavenging free radicals and increasing antioxidant enzyme activities. Practical applications Considerable public and scientific concern was raised when researches have shown the impact of extremely low frequency electromagnetic field (ELF‐EMF) on cognition, nervous system function and brain activity. Catechin and epicatechin are widely distributed in tea, fruits and vegetables. This study highlights that catechin and epicatechin can attenuate these abnormal oxidative damage and related learning and memory deficits induced by ELF‐EMF exposure. Knowledge generated in this study will be beneficial in developing catechin and epicatechin as the candidates for treating the neurological and neurodegenerative disorders induced by ELF‐EMF exposure.

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Exposure to extremely low frequency electromagnetic fields alters the calcium dynamics of cultured entorhinal cortex neurons

Cited by 24 publications

References 57 publications

Stable morphological–physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide

Stable morphological–physiological and neural protein expression changes in rat bone marrow mesenchymal stem cells treated with electromagnetic field and nitric oxide

Neuroprotective effects of lotus seedpod procyanidins on extremely low frequency electromagnetic field-induced neurotoxicity in primary cultured hippocampal neurons

Beneficial effect of catechin and epicatechin on cognitive impairment and oxidative stress induced by extremely low frequency electromagnetic field

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