The relationship between orexin/hypocretin and rapid eye movement (REM) sleep remains elusive. Here, we find that a proportion of orexin neurons project to the sublaterodorsal tegmental nucleus (SLD) and exhibit REM sleep-related activation. In SLD, orexin directly excites orexin receptor-positive neurons (occupying ~3/4 of total-population) and increases gap junction conductance among neurons. Their interaction spreads the orexin-elicited partial-excitation to activate SLD network globally. Besides, the activated SLD network exhibits increased probability of synchronized firings. This synchronized excitation promotes the correspondence between SLD and its downstream target to enhance SLD output. Using optogenetics and fiber-photometry, we consequently find that orexin-enhanced SLD output prolongs REM sleep episodes through consolidating brain state activation/muscle tone inhibition. After chemogenetic silencing of SLD orexin signaling, a ~17% reduction of REM sleep amounts and disruptions of REM sleep muscle atonia are observed. These findings reveal a stabilization role of orexin in REM sleep.

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A courtyard is a traditional and popular construction feature found in China’s urban buildings. This case study evaluates the performance of the traditional courtyard design of the Jiangnan Museum, located in Jiangsu Province. In the evaluation, the spatial layout of courtyards is adjusted, the aspect ratio is changed, and an ecological buffer space is created. To model and evaluate the performance of the courtyard design, this study applied the Computational fluid dynamics (CFD) software, Parabolic Hyperbolic Or Elliptic Numerical Integration Code Series (PHOENICS), for wind environment simulation, and the EnergyPlus-based software, DesignBuilder, for energy simulation. Results show that a good combination of courtyard layout and aspect ratio can improve the use of natural ventilation by increasing free cooling during hot summers and reducing cold wind in winters. The results also show that ecological buffer areas of a courtyard can reduce cooling loads in summer by approximately 19.6% and heating loads in winter by approximately 22.3%. The study provides insights into the optimal design of a courtyard to maximize its benefit in regulating the microclimate during both winter and summer.

The medial entorhinal cortex (MEC) plays a crucial role in spatial learning and memory. Whereas the MEC receives a dense histaminergic innervation from the tuberomamillary nucleus of the hypothalamus, the functions of histamine in this brain region remain unclear. Here, we show that histamine acts via H1Rs to directly depolarize the principal neurons in the superficial, but not deep, layers of the MEC when recording at somata. Moreover, histamine decreases the spontaneous GABA, but not glutamate, release onto principal neurons in the superficial layers by acting at presynaptic H3Rs without effect on synaptic release in the deep layers. Histamine-induced depolarization is mediated via inhibition of Kir channels and requires the activation of protein kinase C, whereas the inhibition of spontaneous GABA release by histamine depends on voltage-gated Ca(2+) channels and extracellular Ca(2+). Furthermore, microinjection of the H1R or H3R, but not H2R, antagonist respectively into the superficial, but not deep, layers of MEC impairs rat spatial learning as assessed by water maze tasks but does not affect the motor function and exploratory activity in an open field. Together, our study indicates that histamine plays an essential role in spatial learning by selectively regulating neuronal excitability and synaptic transmission in the superficial layers of the MEC.