Exposure to High-Fat Style Diet Induced Renal and Liver Structural Changes, Lipid Accumulation and Inflammation in Intact and Ovariectomized Female Rats

Sucedaram, Yamuna; Johns, Edward J.; Husain, Ruby; Sattar, M. A.; Abdulla, Mohammed H.; Giribabu, Nelli; Rahim, Nur Syahrina; Khalilpourfarshbafi, Manizheh; Abdullah, Nor Azizan

doi:10.2147/jir.s299083

Cited by 10 publications

(5 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reportedly, a study proves that HFD intake increases the obese gene product known as leptin, total body fat, and calorie intake ( 23 ). This, in turn, manifests in the changes in histological and morphological changes of organs, liver, and renal markers ( 24 ). Thus, HFD is considered to be one of the most studied methods to induce obesity ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

The role of Elateriospermum tapos yoghurt in mitigating high-fat dietary cause of maternal obesity—an experimental study

et al. 2023

View full text Add to dashboard Cite

Maternal obesity is the key predictor for childhood obesity and neurodevelopmental delay in the offspring. Medicinal plants are considered to be the safe and best option, and at the same time, probiotic consumption during pregnancy provides beneficial effects for both the mother and the child. Current research has shown that Elateriospermum tapos (E. tapos) yoghurt is safe to consume and consists of many bioactive compounds that can exert an anti-obesity effect. Thus, this study has been designed to study the role of E. tapos yoghurt in mitigating maternal obesity. In this study, a total of 48 female Sprague Dawley (SD) rats were assigned to six groups, with eight rats per group, and obesity was induced over 16 weeks with a high-fat diet (HFD) pellet. On the 17th week, the rats were allowed to mate and pregnancy was confirmed through vaginal smear. The obese induced group was further divided into negative and positive control groups, followed by E. tapos yoghurt treatment groups with three different concentrations (5, 50, and 500 mg/kg). The changes in body weight, calorie intake, lipid profile, liver profile, renal profile, and histopathological analysis were measured on postnatal day (PND) 21. The results show that the group with the highest concentration of E. tapos yoghurt (HYT500) supplementation shows gradual reduction in body weight and calorie intake on PND 21 and modulates the lipid level, liver, and renal enzymes to a normal level similar to the normal group. In histological analysis, HYT500 reverses the damage caused by HFD in liver and colon, and reverses the adipocytes’ hypertrophy in retroperitoneal white adipose tissue and visceral fat. In conclusion, supplementation of E. tapos yoghurt during the gestational period up to weaning is effective in the gradual weight loss of maternal obese dams from the 500-mg/kg-supplemented group in this study.

show abstract

Section: Discussionmentioning

confidence: 99%

The role of Elateriospermum tapos yoghurt in mitigating high-fat dietary cause of maternal obesity—an experimental study

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that ovariectomy increases susceptibility to the severity of chronic arthritis and local inflammation because of the resulting lack of estrogen, and also that estradiol-replacement therapy can induce a protective anti-inflammatory effect and improves innate immune responses in ovariectomized arthritic mice ( 54 ). In additional, the structural changes and inflammatory response in the kidney and liver have been shown to be significantly increased by a high-fat diet and exacerbated by ovariectomized conditions ( 55 ). Estrogen is thought to play an important regulatory role in the innate and adaptive immune systems.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Whole-Body Vibration Exercise Combined With an Isocaloric High-Fructose Diet on Osteoporosis and Immunomodulation in Ovariectomized Mice

et al. 2022

View full text Add to dashboard Cite

BackgroundOsteoporosis and immune-associated disorders are highly prevalent among menopausal women, and diet control and exercise exert beneficial effects on physiological modulation in this population. A controlled diet with a low fat content and a balanced caloric intake improves menopausal health, but the health effects of excessive fructose consumption on menopausal women are yet to be confirmed. In addition, whole-body vibration (WBV), a safe passive-training method, has been shown to have multiple beneficial effects on metabolism regulation, obesity, and bone health.MethodsThe ovariectomized (OVX) C57BL/6J model was used to verify the effects of WBV combined with a high-fructose diet (HFrD) for 16 weeks on physiological modulation and immune responses. The mice were randomly allocated to sham, OVX, OVX+HFrD, and OVX+HFrD+WBV groups, which were administered with the indicated ovariectomy, dietary and WBV training treatments. We conducted growth, dietary intake, glucose homeostasis, body composition, immunity, inflammation, histopathology, and osteoporotic assessments (primary outcomes).ResultsOur results showed that the isocaloric HFrD in OVX mice negated estrogen-deficiency–associated obesity, but that risk factors such as total cholesterol, glucose intolerance, osteoporosis, and liver steatosis still contributed to the development of metabolic diseases. Immune homeostasis in the OVX mice was also negatively affected by the HFrD diet, via the comprehensive stimulation of T cell activation, causing inflammation. The WBV intervention combined with the HFrD model significantly ameliorated weight gain, glucose intolerance, total cholesterol, and inflammatory cytokines (interferon gamma [IFN-γ], interleukin [IL]-17, and IL-4) in the OVX mice, although osteoporosis and liver steatosis were not affected compared to the negative control group. These findings indicate that an isocaloric high-fructose diet alone may not result in menopausal obesity, but that some deleterious physiological impacts still exist.ConclusionThe WBV method may modulate the physiological impacts of menopause and the HFrD diet, and should be considered as an alternative exercise prescription for people with poor compliance or who are unable or unwilling to use traditional methods to improve their health. In future studies, using the WBV method as a preventive or therapeutic strategy, combined with nutritional interventions, medication, and other exercise prescriptions, may prove beneficial for maintaining health in menopausal women.

show abstract

“…The double immunofluorescence was performed according to the method as previously described. 33,34 The deparaffinised, rehydrated and antigen retrieved sections were blocked with 2.5% normal horse serum provided in the kit (Vector Laboratories, Burlingame, CA, USA) for 30 min at room temperature. The slides were incubated with first primary antibodies like caspase-3 (NB100-56708; Novus Biologicals, Littleton, CO, USA) at ratio of 1:1000 for 2 h at room temperature, then the slides were washed with PBS for three times.…”

Section: Methodsmentioning

confidence: 99%

Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice

et al. 2021

View full text Add to dashboard Cite

Background Diabetes is a serious global health concern which severely affected public health as well as socio-economic growth worldwide. Scutellarin (SCU), a bioactive flavonoid, is known for its efficacious action against a range of ailments including cardiovascular problems. The present study was conducted to find out possible protective effect and its associated mechanisms of SCU on experimental type 2 diabetes-induced cardiac injury. Methods Type 2 diabetes was induced by treating animals with high fat diet for 4 weeks and a single intraperitoneal dose (35 mg/kg body weight) of streptozotocin and diabetic animals received SCU (10 or 20 mg/kg/day) for 6 weeks. Results Scutellarin attenuated type 2 diabetes-induced hyperglycemia, bodyweight loss, hyperlipidaemia, cardiac functional damage with histopathological alterations and fibrosis. Scutellarin treatment to type 2 diabetic mice ameliorated oxidative stress, inflammatory status and apoptosis in heart. Furthermore, the underlying mechanisms for such mitigation of oxidative stress, inflammation and apoptosis in heart involved modulation of Nrf2/Keap1 pathway, TLR4/MyD88/NF-κB mediated inflammatory pathway and intrinsic (mitochondrial) apoptosis pathway, respectively. Conclusions The current findings suggest that SCU is effective in protecting type 2 diabetes-induced cardiac injury by attenuating oxidative stress and inflammatory responses and apoptosis, and it is also worth considering the efficacious potential of SCU to treat diabetic cardiomyopathy patients.

show abstract

Exposure to High-Fat Style Diet Induced Renal and Liver Structural Changes, Lipid Accumulation and Inflammation in Intact and Ovariectomized Female Rats

Cited by 10 publications

References 90 publications

The role of Elateriospermum tapos yoghurt in mitigating high-fat dietary cause of maternal obesity—an experimental study

The role of Elateriospermum tapos yoghurt in mitigating high-fat dietary cause of maternal obesity—an experimental study

The Effects of Whole-Body Vibration Exercise Combined With an Isocaloric High-Fructose Diet on Osteoporosis and Immunomodulation in Ovariectomized Mice

Scutellarin alleviates type 2 diabetes (HFD/low dose STZ)-induced cardiac injury through modulation of oxidative stress, inflammation, apoptosis and fibrosis in mice

Contact Info

Product

Resources

About