Exposure to welding fumes activates DNA damage response and redox-sensitive transcription factor signalling in Sprague-Dawley rats

Krishnaraj, Jayaraman; Kowshik, Jaganathan; Sebastian, Robin; Raghavan, Sathees C.; Nagini, Siddavaram

doi:10.1016/j.toxlet.2017.04.001

Cited by 14 publications

(7 citation statements)

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“…Similar results were obtained in the studies with 20 volunteers from local welding apprentice schools in Switzerland (Graczyk et al, 2016) and 238 welders in Germany (Pesch et al, 2015). Also, 8-OHdG levels were found to be higher in animals exposed to welding fumes (Krishnaraj et al, 2017; Yu et al, 2004).…”

Section: Discussionmentioning

confidence: 95%

Oxidative stress status of Turkish welders

et al. 2020

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Welding, a fabrication process that joins metals or thermoplastics by causing coalescence, is indispensable in modern society and ubiquitous in industry. Welding generates fumes that contain several metals and gases that comprise fine and ultrafine particles with the potential for adverse effects. Although health risks of welders have been evaluated in different populations, occupational exposure to welding fumes is still considered to be an important health problem, especially in developing countries. The aim of this study was to investigate the effects of welding fume exposure on important oxidative stress parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), total glutathione (GSH), glutathione peroxidase (GPx), malondialdehyde (MDA), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in Turkish welders ( n = 48). The influence of confounding factors such as age, smoking habits, alcohol consumption, and duration of exposure on the studied parameters was also analyzed. In our study, significant decreases in the levels of GSH and activities of CAT, SOD, and GPx and significant increases of MDA, 8-OHdG levels and GR activity were found in the workers compared to the controls. There was a negative correlation between GSH levels and alcohol usage. Also, older workers (≥35 years) had significantly higher GR levels than younger workers. But smoking and alcohol usage, duration of exposure, and utilization of protective measures had no significant effect on the studied parameters in the workers. These results indicate that occupational exposure to welding fumes appears to induce oxidative stress and oxidative DNA damage.

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Section: Discussionmentioning

confidence: 95%

Oxidative stress status of Turkish welders

et al. 2020

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“…In this study, we have investigated the effects of repeated inhalation SS welding dust (WD) and its soluble form (SWD) on club (Clara) cells in the bronchiolar epithelium and Clara cell protein (CC16) in rats and inquired whether the nuclear factor-kappa B (NF-κB) and or c-Jun/AP-1 related pathways were involved. Metals present in welding fumes of stainless steels including Cr(VI) and nickel-induced generation of reactive oxygen species (ROS) [4,15,33]. Some study certificate correlation among Cr(VI)-induced generation of ROS and neoplasia process [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

“…At the molecular level, the primary adaptive response to welding fume exposure may be the activation of Nrf2 and NF-κB signaling pathways in activating the response to ROS and DNA damage. [4]. Meyers' [40] review shows that Cr(VI) generates irreversible setback of thioredoxin reductase (TrxR) and oxidation of peroxiredoxin (Prx) and thioredoxin (Trx).…”

Section: Discussionmentioning

confidence: 99%

“…migration, apoptosis, inflammation, and fibrosis. Reactive oxygen species elicit these effects by influencing intracellular signaling events [4]. The carcinogenic effect of Cr(VI) and Ni 2+ and the neuroinflammatory action of Mn has been associated with activation of mostly redox-sensitive transcription factors, engaging nuclear factor-kappa B (NF-κB), activator protein 1 (AP-1) and p53 and further signaling factors [16][17][18][19][20].…”

Section: Ijomeh 2018;31(5)mentioning

confidence: 99%

“…Welding fumes of stainless steel containing in particular chromium (Cr(VI)) and nickel (Ni) as possible carcinogens to welders have a high priority for risk assessment. In the toxicological evaluation of the effects of exposure to welding fumes of stainless steels (SS welding) studies by animal/ rats inhalation, intra tracheal implementation of collected welding dust or in vitro studies were performed [4][5][6][7]. Cena et al [8] showed that the samples fraction below 300 nm of welding stainless fume contained the majority of Cr and Ni and manganese (Mn) in the entire welding fumes.…”

Section: Introductionmentioning

confidence: 99%

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Clara cells protein, prolactin and transcription factors of protein NF-ĸB and c-Jun/AP-1 levels in rats inhaled to stainless steel welding dust and its soluble form

Hałatek

Stanislawska

Świercz

et al. 2018

Int J Occup Med Environ Health

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Objectives: Welding processes that generate fumes containing toxic metals, such as hexavalent chromium (Cr(VI)), manganese, and nickel (Ni), have been implicated in lung injury, inflammation, and lung tumor promotion in animal models. Bronchiolar epithelium Clara cells/club cells, coordinate these inflammatory responses. Clara cells secretory protein (CC16) with ant-inflammatory role. Material and Methods: The pulmonary toxicity of welding dust (WD) was assessed for Wistar rats exposed to 60 mg/m 3 of respirable-size welding dust (mean diameter 1.17 μm for 1 and 2 weeks (6 h/day, 5 days/week)) or the aerosols of soluble form (SWD) in the nose-only exposure chambers. Additionally the effect of antiinflammatory betaine supplementation was assessed. Clara cells secretory protein, differential cell counts, total protein concentrations and cellular enzyme (lactate dehydrogenase-LDH) activities were determined in bronchoalveolar lavage fluid, and corticosterone and thiobarbituric acid reactive substances (TBARS) and prolactin concentrations were assessed in serum. Histopathology examination of lung, brain, liver, kidney, spleen was done. Additionally slices of brain and lung were exanimated in laser ablation inductively coupled plasma mass spectrometry. Results: Both WD and SWD exposure evoked large bronchiolar infiltration shoved in histopathology examination. In this study, TBARS inversely correlated with a significant decrease of CC16 concentration that occurred after instillation of both WD and SWD indicating decreased anti-inflammatory potential in the lung. In WD exposed rats prolactin correlated with nuclear factor-kappa B (NF-κB), LDH, TBARS and serum levels Cr, Ni and inversely with c-Jun. In SWD exposed rats prolactin correlated with CC16 indicated effect of prolactin on the population of epithelial cells. Conclusions: In the current study, deleterious effects of repeated inhalation stainless steel welding dust form on club (Clara) cell secretory protein (CC16) were demonstrated. Clara cells secretory protein relation with prolactin in exposed rats to welding dust were shown and explored whether the NF-κB and c-Jun/activator protein 1 related pathway was involved.

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