Expresión diferencial en placenta de beta-defensinas humanas y detección de variantes alélicas en el gen DEFB1 de madres positivas para VIH-1

Aguilar-Jiménez, Wbeimar; Zapata, Wildeman; Rugeles, Marı́a Teresa

doi:10.7705/biomedica.v31i1.335

Cited by 11 publications

(2 citation statements)

References 70 publications

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“…Indeed, a protective role for human ß-defensins against HIV acquisition in high risk exposed individuals has been suggested by Rugeles and colleagues (Zapata et al, 2008;Aguilar-Jimenez et al, 2013), and hBD2 has been shown to be correlated with ant-HIV activity in cervical-vaginal secretions (Ghosh et al, 2010;Patel et al, 2014). Several studies have also demonstrated an association between different ß-defensin gene polymorphisms and HIV infection in adults as well as children of diverse ethnicities (Braida et al, 2004;Milanese et al, 2006;Baroncelli et al, 2008;Milanese et al, 2009;Ricci et al, 2009;Aguilar-Jimenez et al, 2011;Freguja et al, 2012;Hardwick et al, 2012). Human ß-defensins, especially hBD2, therefore merit further consideration as both an immunotherapeutic and topical microbicide to prevent spread through sexual contact.…”

Section: Discussionmentioning

confidence: 96%

Human Beta-Defensin 2 and 3 Inhibit HIV-1 Replication in Macrophages

Bharucha

Sun

et al. 2021

Front. Cell. Infect. Microbiol.

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Human beta-defensins (hBDs) are broad-spectrum antimicrobial peptides, secreted by epithelial cells of the skin and mucosae, and astrocytes, which we and others have shown to inhibit HIV-1 in primary CD4+ T cells. Although loss of CD4+ T cells contributes to mucosal immune dysfunction, macrophages are a major source of persistence and spread of HIV and also contribute to the development of various HIV-associated complications. We hypothesized that, besides T cells, hBDs could protect macrophages from HIV. Our data in primary human monocyte-derived macrophages (MDM) in vitro show that hBD2 and hBD3 inhibit HIV replication in a dose-dependent manner. We determined that hBD2 neither alters surface expression of HIV receptors nor induces expression of anti-HIV cytokines or beta-chemokines in MDM. Studies using a G-protein signaling antagonist in a single-cycle reporter virus system showed that hBD2 suppresses HIV at an early post-entry stage via G-protein coupled receptor (GPCR)-mediated signaling. We find that MDM express the shared chemokine-hBD receptors CCR2 and CCR6, albeit at variable levels among donors. However, cell surface expression analyses show that neither of these receptors is necessary for hBD2-mediated HIV inhibition, suggesting that hBD2 can signal via additional receptor(s). Our data also illustrate that hBD2 treatment was associated with increased expression of APOBEC3A and 3G antiretroviral restriction factors in MDM. These findings suggest that hBD2 inhibits HIV in MDM via more than one CCR thus adding to the potential of using β-defensins in preventive and therapeutic approaches.

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Section: Discussionmentioning

confidence: 96%

Human Beta-Defensin 2 and 3 Inhibit HIV-1 Replication in Macrophages

Bharucha

Sun

et al. 2021

Front. Cell. Infect. Microbiol.

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“…For example, in positive HIV mothers, it has been seen that the expression of hBD-1 in the placenta is higher than in negative HIV mothers, but the role of HIV-1 in the induction of hBD-1 in the placenta has not been explored. It has been hypothesized that single nucleotide polymorphisms (SNPs) in the hBD-1 gene are linked to the likelihood of HIV-1 infection ( Aguilar-Jiménez et al, 2011 ).…”

Section: Roles In the Human Reproduction Systemmentioning

confidence: 99%

Roles of Different β-Defensins in the Human Reproductive System: A Review Study

et al. 2023

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Human β-defensins (hBDs) are cationic peptides with an amphipathic spatial shape and a high cysteine content. The members of this peptide family have been found in the human body with various functions, including the human reproductive system. Of among β-defensins in the human body, β-defensin 1, β-defensin 2, and β-defensin 126 are known in the human reproductive system. Human β-defensin 1 interacts with chemokine receptor 6 (CCR6) in the male reproductive system to prevent bacterial infections. This peptide has a positive function in antitumor immunity by recruiting dendritic cells and memory T cells in prostate cancer. It is necessary for fertilization via facilitating capacitation and acrosome reaction in the female reproductive system. Human β-defensin 2 is another peptide with antibacterial action which can minimize infection in different parts of the female reproductive system such as the vagina by interacting with CCR6. Human β-defensin 2 could play a role in preventing cervical cancer via interactions with dendritic cells. Human β-defensin 126 is required for sperm motility and protecting the sperm against immune system factors. This study attempted to review the updated knowledge about the roles of β-defensin 1, β-defensin 2, and β-defensin 126 in both the male and female reproductive systems.

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Treating the Placenta

Reed¹,

Mattison²

2013

Clinical Pharmacology During Pregnancy

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Expresión diferencial en placenta de beta-defensinas humanas y detección de variantes alélicas en el gen DEFB1 de madres positivas para VIH-1

Cited by 11 publications

References 70 publications

Human Beta-Defensin 2 and 3 Inhibit HIV-1 Replication in Macrophages

Human Beta-Defensin 2 and 3 Inhibit HIV-1 Replication in Macrophages

Roles of Different β-Defensins in the Human Reproductive System: A Review Study

Treating the Placenta

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