Expressed Proteins of Herbaspirillum seropedicae in Maize (DKB240) Roots-Bacteria Interaction Revealed Using Proteomics

Abstract

Several molecular tools have been used to clarify the basis of plant-bacteria interaction; however, the mechanism behind the association is still unclear. In this study, we used a proteomic approach to investigate the root proteome of Zea mays (cv. DKB240) inoculated with Herbaspirillum seropedicae strain SmR1 grown in vitro and harvested 7 days after inoculation. Eighteen differentially accumulated proteins were observed in root samples, ten of which were identified by MALDI-TOF mass spectrometry peptide mass fingerprint. Among the identified proteins, we observed three proteins present exclusively in inoculated root samples and six upregulated proteins and one downregulated protein relative to control. Differentially expressed maize proteins were identified as hypothetical protein ZEAMMB73_483204, hypothetical protein ZEAMMB73_269466, and tubulin beta-7 chain. The following were identified as H. seropedicae proteins: peroxiredoxin protein, EF-Tu elongation factor protein, cation transport ATPase, NADPH:quinone oxidoreductase, dinitrogenase reductase, and type III secretion ATP synthase. Our results presented the first evidence of type III secretion ATP synthase expression during H. seropedicae-maize root interaction.

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

“…Investigations describing the biological response of plants following the inoculation of diazotrophic bacteria in maize are scarce [42,43], although there have been more on other grasses, notably sugaracane [68,69]. However, it has been established that growth promoting and diazotrophic bacteria have a strong impact on both the plant transcriptome and metabolome, reflecting in certain cases the improved performance of the inoculated plants [70,71].…”

Leaf proteome comparison of two GM common bean varieties and their non‐GM counterparts by principal component analysis

“…Second‐dimension SDS‐PAGE gels were run in an SE 600 Ruby System (GE Healthcare) under 15 mA per gel for 30 min and 30 mA per gel for 4 h. The temperature was kept at 10 °C using a MultiTemp III thermostatic circulator (GE Healthcare). Protein spots were visualized by staining the gels with 0.01 g L −1 Coomassie Brilliant Blue G‐250 (Bio‐Rad, Hercules, CA, USA) as described previously …”

Proteomic analysis of salt‐responsive proteins in oat roots (Avena sativa L.)

“…Transcriptomic approaches are important, as quantitative analysis of gene expression at the protein level is essential for determining plant responses to salt stress. 11 In recent years, high-resolution protein separation using two-dimensional gel electrophoresis , coupled with protein identification using mass spectrometry, has become a powerful tool to study plant proteomics. 4 Variations in plant proteome under salt stress and other conditions have been reported in many plants, including rice, wheat, tomato, barley and maize.…”

“…In recent years, high‐resolution protein separation using two‐dimensional gel electrophoresis (2‐DE), coupled with protein identification using mass spectrometry, has become a powerful tool to study plant proteomics . Variations in plant proteome under salt stress and other conditions have been reported in many plants, including rice, wheat, tomato, barley and maize . Most of these studies were based on short‐term salt stress (i.e., 0.5 to 48 hours), while some were based on longer‐term salt stress .…”