Expressed sequence tags (ESTs) based identification of genes and expression analysis of leukocyte cell-derived chemotaxin-2 (LECT2) from Epinephelus bruneus

Harikrishnan, Ramasamy; Kim, Ju-Sang; Kim, Man-Chul; Balasundaram, Chellam; Heo, Moon‐Soo

doi:10.1016/j.gene.2011.08.029

Cited by 9 publications

(3 citation statements)

References 60 publications

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“…Moreover, three disulphide bonds exist between six evolutionarily conserved cysteine residues in mammals, cyprinids and catfish (1,23,37). However, consistent with most teleost species (19,22,37), On-LECT2 contains four conserved cysteine residues and lacks the second and third cysteine residues, implying that LECT2 might had been passed down through several generations and modified in most fish lineages. Similar to higher vertebrates and other fish lineages (18,23,24,38), the highest transcriptional level of On-lect2 was detected in the liver, whereas the lower levels were observed in the other tissues.…”

Section: Discussionmentioning

confidence: 98%

“…This result highlighted the evolutionary importance of LECT2 because it is highly conserved across a wide variety of species. Moreover, three disulphide bonds exist between six evolutionarily conserved cysteine residues in mammals, cyprinids and catfish ( 1 , 23 , 37 ). However, consistent with most teleost species ( 19 , 22 , 37 ), On-LECT2 contains four conserved cysteine residues and lacks the second and third cysteine residues, implying that LECT2 might had been passed down through several generations and modified in most fish lineages.…”

Section: Discussionmentioning

confidence: 99%

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LECT2 Protects Nile Tilapia (Oreochromis niloticus) Against Streptococcus agalatiae Infection

Zhang

Fan

et al. 2021

Front. Immunol.

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Leukocyte cell-derived chemotaxin 2 (LECT2) is a multifunctional cytokine that especially plays an important role in innate immune. However, the roles of LECT2 in the immune response of the economically important fish Nile tilapia (Oreochromis niloticus) against bacterial infection remains unclear. In this study, a lect2 gene from Nile tilapia (On-lect2) was identified, and its roles in the fish’s immune response against bacterial infection were determined and characterised. On-lect2 contains an open reading frame of 456 bp that encodes a peptide of 151 amino acids, as well as the conservative peptidase M23 domain. On-LECT2 is 62%–84% identical to other fish species and about 50% identical to mammals. The highest transcriptional level of On-lect2 was detected in the liver, whereas the lowest levels were detected in the other tissues. Moreover, the On-LECT2 protein is located mainly in the brain and head kidney. The transcriptional levels of On-lect2 substantially increased in the head kidney, brain, liver and spleen after Streptococcus agalactiae infection. Knockdown On-lect2 led to higher mortality due to liver necrosis or haemorrhage and splenomegaly. In vitro analysis indicated that the recombinant protein of On-LECT2 improved phagocytic activity of head kidney-derived macrophages. In vivo challenge experiments revealed several functions of On-LECT2 in the immune response of Nile tilapia against bacterial infection, including promotion of inflammation, reduction of tissue damages and improvement of survival rate.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

LECT2 Protects Nile Tilapia (Oreochromis niloticus) Against Streptococcus agalatiae Infection

Zhang

Fan

et al. 2021

Front. Immunol.

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“…白细胞衍生趋化因子 2 (leukocyte cell-derived chemotaxin 2, LECT2)是一个多功能细胞因子，参与肝移植及再生 (Sato et al, 2004;Ohtomi et al, 2007)、类风湿性关节炎 (Okumura et al, 2008)、肾淀粉样病变 (Larsen et al, 2010)以及肿瘤细胞生长和迁移 (Ong et al, 2011)等多种病理和生理过程。近年来，鱼类 LECT2 的研究发展迅速，且主要集中于其与病原微生物感染免疫反应的相关性。目前已克隆了鲤鱼 (Cyprinus carpio) (Fujiki et al, 2000) 、虹鳟 (Oncorhynchus mykiss, rainbow trout) (Bayne et al, 2001)、斑马鱼(Danio rerio) (Lin et al, 2007)、大黄鱼(Pseudosciaena crocea) )、香鱼 (Plecoglossus altivelis) (Yang et al, 2009)、赤点石斑鱼(Epinephelus akaara) )、斜带石斑鱼 (E. coioides) (Wei et al, 2011)和褐石斑鱼(E. bruneus) (Harikrishnan et al, 2012) Figure 1 Purification of recombinant rainbow trout LECT2 using cation-exchange chromatography A：重组虹鳟 LECT2 UNOsphere S 阳离子交换柱分离纯化洗脱峰；B：洗脱峰 SDS-PAGE 检测; C：洗脱峰 Western blot 检测; 1: 洗脱峰 1; 2: 洗脱峰 2。…”

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Yeast-based production, purification and bioactivity assay of rainbow trout LECT2

Shi

Zhang²,

Yang³

et al. 2013

Zoological Research

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Leukocyte cell-derived chemotaxin 2 (LECT2) is a secretory cytokine that functions in many physiological and pathological processes. We used a Pichia pastoris expression system for the recombinant expression of rainbow trout LECT2. The recombinant LECT2 was purified by UNOsphere S Cation exchange and size-exclusion chromatography columns. The obtained target protein was highly pure (>96% homogeneity) and the yield was >120 mg/L of yeast cultures. An in vitro chamber assay revealed that recombinant LECT2 could induce chemotactic responses in rainbow trout head kidney-derived macrophages. Recombinant LECT2 not only enhanced macrophage respiratory burst activity and bactericidal activity, but also changed macrophage gene expression. In summary, we established a rapid and efficient method to prepare active recombinant rainbow trout LECT2 using a yeast expression system and column chromatography. Bioactive recombinant LECT2 is essential for studies on protein functions.

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Sequencing of the first ayu (Plecoglossus altivelis) macrophage transcriptome and microarray development for investigation the effect of LECT2 on macrophages

Hang

Yin

et al. 2013

Fish & Shellfish Immunology

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Expressed sequence tags (ESTs) based identification of genes and expression analysis of leukocyte cell-derived chemotaxin-2 (LECT2) from Epinephelus bruneus

Cited by 9 publications

References 60 publications

LECT2 Protects Nile Tilapia (Oreochromis niloticus) Against Streptococcus agalatiae Infection

LECT2 Protects Nile Tilapia (Oreochromis niloticus) Against Streptococcus agalatiae Infection

Yeast-based production, purification and bioactivity assay of rainbow trout LECT2

Sequencing of the first ayu (Plecoglossus altivelis) macrophage transcriptome and microarray development for investigation the effect of LECT2 on macrophages

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