Expression analyses of digestive enzymes during early development and in adults of the chame fishDormitator latifrons

Abstract

This study describes the expression of genes that encode digestive enzymes (trypsin, pepsinogen, maltase-glucoamylase, sucrase-isomaltase, alkaline phosphatase and leucine aminopeptidase) using qRT-PCR in 0-to 7-day post-hatching (dph) chame (Dormitator latifrons). Additionally, adults liver and intestine were analysed for enzyme expression.Results showed that transcripts for all enzymes are present in both adult and larvae tissues. In adults, the expression of maltase-glucoamylase and sucrose-isomaltase showed the highest values in the middle intestine, while the liver and anterior and posterior intestine showed low expression levels. Other enzymes showed low expression in all tissues, with the exception of leucine aminopeptidase, which showed high expression in liver. In larvae whole-body samples of D. latifrons, maltase-glucoamylase and sucrose-isomaltase showed the highest expression from 3 to 5 dph, while other enzymes maintained low levels from hatching to 7 dph. The highest expression of disaccharidases such as maltaseglucoamylase and sucrose-isomaltase corresponded to the beginning of exogenous feeding, suggesting that this species exhibits an herbivorous profile. However, the presence of proteolytic enzymes may indicate that D. latifrons is programmed to be able to digest other substrates. This information will contribute to the development of larval feeding protocols to produce chame juveniles in laboratory conditions, as prior larval feeding essays have not been successful, using either live feeds or commercial fish larval diets. K E Y W O R D S chame, digestive enzyme, Dormitator latifrons, gene expression How to cite this article: Morelos-Castro, Román-Reyes JC, Luis-Villaseñor IE, Ramírez-Pérez JS, Rodríguez-Montes de Oca GA. Expression analyses of digestive enzymes during early development and in adults of the chame fish Dormitator latifrons. Aquac Res. 2020;51:265-275. https ://doi.

Development of digestive enzymatic activity and gene expression during the early ontogeny of Chinese perch (Siniperca chuatsi)

“…Almost digestive enzyme genes expression (excepted amy and pep) were detected at hatching, it's concurrent with the digestive enzyme activities. The expression of try in S. chuatsi larvae were detected at hatching and stained slight fluctuations until 15 dph, these results agree with other reports in larvae of several fish species [1,11,26] . Then, the expression of try gradually decreased until 30 dph, similar findings about try expression have been reported in other species such as Lates calcarifer [3] , Clarias magur [8] , Lutjanus guttatus [27] and…”

“…showed that the expression of alp gene was detected at hatching, but it remained a very low level until 10 dph. Similar pattern has been observed in Dormitator latifrons [11] , the expression of alp was not high at early stage. This might be related to the development of the gut of fish, as well as other genes involved in the development of the digestive tract such as leucine aminopeptidase (lap).…”

“…It is necessary to evaluate the comprehensively digestive ability of S. chuatsi larva from the aspects of gene expression and enzyme activity detection. Recently, the use of molecular biological approaches have complemented traditional methods in expressing the profiles of digestive enzyme precursors in fish [1,8,11,12] and thus providing insight into both temporal and spatial expression patterns of genes involved in the development and functionality of digestive systems during early ontogeny. As such, this study looks to investigate the early ontogeny of S. chuatsi through an integrative investigation to understand the general patterns of activity through the effect of feeding status, morphological changes, and gene expression on final enzymatic capacity.…”

Understanding the early ontogenetic stages of Mugil liza (Mugilidae): Morphological traits and digestive/metabolic profile of pre‐juveniles after recruitment

“…Data are the mean ± activity was found in early and late pre-juveniles. This suggests that this activity could correspond to a sucrase-independent maltaseglucoamylase complex (Bertucci et al, 2019;Brun et al, 2020;Chaudet et al, 2019;Dhital et al, 2013;Morelos-Castro et al, 2020).…”

Contribution to some aspects of the digestive tract anatomy and food evacuation of the Pacific fat sleeper Dormitator latrifrons

“…However, the functions and capabilities of fish stomachs have changed (they have even been lost in some species) as fish evolved (Castro et al 2014). As is the case of some species of the sleeper family, such as D. latifrons, which, according to some reports, lacks a true stomach and has detritivorous (Morelos-Castro et al 2019) and omnivorous habits too (Freire-Lascano 2016). In general, the study of the passage of food through the digestive tract of fish suggests that, in addition to the specific anatomical-physiological characteristics of each species, there are factors that influence the transit time of food, such as culture temperature, stress, intestinal size and length, body size, hardness and composition of diets (Logothetis et al 2001, Booth et al 2008, Uscanga et al 2010, Andersen 2012, Khan & Seyhan 2019.…”