Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera

Li, Zhaoqun; Zhang, Shuai; Luo, Junyu; Zhu, Jing; Cui, Jinjie; Dong, Shuang-Lin

doi:10.1007/s10886-015-0574-x

Cited by 46 publications

(43 citation statements)

References 56 publications

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“…3), which implied their function in chemoreception in this period 39, 40 . Consistent with our results here, a previous RNA sequencing (RNAseq) study in S. litura revealed that the expression levels of many SlitCSPs could be enhanced in whole insect bodies, including thorax, wings, labial palps, tarsi, proboscis, pheromone glands and ejaculatory ducts 38, 41, 42 . One interpretation of those data is that SlitCSPs are functional in nonhead tissues where they are used in noncanonical chemosensory roles.…”

Section: Discussionsupporting

confidence: 93%

Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura

Zhou

et al. 2017

Sci Rep

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While it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. Insect chemosensory proteins (CSPs) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. Recent studies have identified a number of CSPs expressed in midgut in Lepidoptera insects, which started to reveal their roles in chemical recognition and stimulating appetite in midgut. In this study, we examined expression patterns in midgut of 21 Spodoptera litura CSPs (SlitCSPs) characterized from a previously reported transcriptome, and three CSPs were identified to be expressed highly in midgut. The orthologous relationships between midgut expressed CSPs in S. litura and those in Bombyx mori and Plutella xylostella also suggest a conserved pattern of CSP expression in midgut. We further demonstrated that the expression of midgut-CSPs may change in response to different host plants, and SlitCSPs could bind typical chemicals from host plant in vitro. Overall, our results suggested midgut expressed SlitCSPs may have functional roles, likely contributing to specialization and adaption to different ecosystems. Better knowledge of this critical component of the chemsensation signaling pathways in midguts may improve our understanding of food preference processes in a new perspective.

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Section: Discussionsupporting

confidence: 93%

Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura

Zhou

et al. 2017

Sci Rep

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“…One CSP ( CSP13 ) was highly expressed in both antennae and mouthparts. The percentage (71.42%) of CSPs that were highly expressed in chemosensory organs of P. americana was greater than in many other insect species; for example, one of five (20%) in Sitobion avenae (Xue et al ., ), two of 17 (11.76%) in Hyphantria cunea (Sanyal et al ., ), two of six (33.33%) in Apis cerana (Li et al ., ), five of 22 (22.72%) in Cnaphalocrocis medinalis (Liu et al ., ) and three of 27 (11.11%) in Helicoverpa armigera (Li et al ., ). Thus, in addition to PameOBPs , many PameCSPs may also play important roles in the chemical perception of P. americana .…”

Section: Resultsmentioning

confidence: 99%

Identification of odorant‐binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana

Zhang

et al. 2017

Insect Molecular Biology

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The American cockroach (Periplaneta americana) is an urban pest with a precise chemosensory system that helps it achieve complex physiological behaviours, including locating food and mating. However, its chemosensory mechanisms have not been well studied. Here, we identified 71 putative odorant carrier protein genes in P. americana, including 57 new odorant-binding proteins (OBPs) and 11 chemosensory proteins (CSPs). To identify their physiological functions, we investigated their tissue expression patterns in antennae, mouthparts, legs, and the remainder of the body of both sexes, and determined that most of these genes were expressed in chemosensory organs. A phylogenetic tree showed that the putative pheromone-binding proteins of P. americana were in different clades from those of moths. Two genes, PameOBP24 and PameCSP7, were expressed equally in antennae of both sexes and highly expressed amongst the OBPs and CSPs. These genes were expressed in Escherichia coli and the resultant proteins were purified. The binding affinities of 74 common odorant compounds were tested with recombinant PameOBP24 and PameCSP7. Both proteins bound a variety of ligands. Our findings provide a foundation for future research into the chemosensory mechanisms of P. americana and help in identifying potential target genes for managing this pest.

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“…The two most abundant CSPs have different sex-specific expression patterns in antennae of Bombyx mori 47. HarmCSP6, sharing 84.4% homology and closely clustered with MS|comp149356_c0, was reported to be highly transcribed in pheromone glands and display high binding affinity for pheromone components48. MS|comp158640_c0 and MS|comp149055_c0 shared 58.9% and 89.7% identity with HarmCSP4 which was detected to be exclusively present in proboscis and could help solubilizing terpenoids present in flower nectar45.…”

Section: Discussionmentioning

confidence: 99%

Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata

Liu

Wang

Lei

et al. 2017

Sci Rep

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Sensory system plays important roles in a wide array of insect’s behavior and physiological events, including the host landing and locating, feeding, flying, sex responding, mating and oviposition which happen independently and in sequence. The armyworm Mythimna separata (Lepidoptera: Noctuidae) of migratory insect is destructive for alimentarn crop and economic crop throughout the world. Here we present the high throughput sequencing of the head transcriptome and identify members of the major sensory genes which are crucial for armyworm’s success worldwide, including 8 opsins, 22 chemosensory proteins, 50 odorant binding proteins, 60 odorant receptors, 8 gustatory receptors, 24 ionotropic receptors, and 2 sensory neuron membrane proteins. It is worth noting that a duplication of the LW opsin gene exists in this insect. Several genes were clustered with functionally validated genes, such as Co-receptors of OR and IR, PBPs, PRs, CO2 GRs, bitter GRs and sweet GRs, were also identified. The transcriptome gene library provided the basis for further studies that elucidate the fundamental molecular mechanism of biology and control in M. separata. Our research exhibits the first comprehensive catalogue of the sensory genes fundamental for success and distribution in M. separata, which are potential novel targets for pest control strategies.

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Expression Analysis and Binding Assays in the Chemosensory Protein Gene Family Indicate Multiple Roles in Helicoverpa armigera

Cited by 46 publications

References 56 publications

Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura

Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura

Identification of odorant‐binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana

Sensory genes identification with head transcriptome of the migratory armyworm, Mythimna separata

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