Expression analysis of a MATE-type transporter gene ofArabidopsisand its orthologues in rice and chickpea under salt stress

Abstract

Salinity is one of the major abiotic stresses that adversely affect the crop growth and productivity. Salt responsive genes belonging to MATE efflux proteins reportedly play a significant role imparting salt tolerance to plants. In the present study, AT5G52050, a putative salt responsive gene from Arabidopsis thaliana encoding MATE efflux family protein which functions as an antiporter and its orthologues in rice (LOC_Os02g45380) and chickpea (LOC101489496) have been identified. The expression pattern of these genes was validated by quantitative reverse transcription polymerase chain reaction. In silico analysis of the upstream promoter regions of these genes revealed the presence of several conserved sequence motifs related to salt response either in single or in multiple copies. A phylogenetic analysis to understand the evolutionary relationship of MATE family genes in Arabidopsis, rice and chickpea revealed conservation of MATE family genes between dicots and monocots. The genes identified in this study may serve as promising candidates for further elucidation of the salt tolerance mechanism in plants.

Genome-wide identification and functional prediction of salt- stress related long non-coding RNAs (lncRNAs) in chickpea (Cicer arietinum L.)

“…It was observed that miRNA: car-miRNA015, Cat-miR159g-3p and Cat-miR172c.2 were targeting auxin response mutant (AXR4), Myb and Ethylene-responsive AP2 transcription factors respectively under stress along with several other interaction is shown in Table S9. We observed that several target genes of miRNAs were reported to play important role in salt stress like MATE efflux family protein (Nimmy et al 2015 ), Pentatricopeptide repeat-containing protein (Jiang et al 2015 , Chen et al 2018 ), Peroxidase (M'barek et al 2017 , Jin et al 2019 ), Ribonuclease (Zheng et al 2014 ), Squamosa promoter-binding-like protein (Hou et al 2018 , Wang et al 2019a ). This shows that identified lncRNAs in this study, play important role in indirect regulation of various salt stress genes.…”

“…Serval other eTMs like, MATE efflux family protein were also playing significant role under salt stress. MATE gene family mostly encodes transporter genes involved in various physiological process in plants (Nimmy et al 2015 ). Role and function of MATE efflux family under abiotic stress has been studied in various other crops like, rice (Du et al 2021 ), soybean (Liu et al 2016 ), tomato (Santos et al 2017 ), etc.…”

Overexpression of Grain Amaranth (Amaranthus hypochondriacus) AhERF or AhDOF Transcription Factors in Arabidopsis thaliana Increases Water Deficit- and Salt-Stress Tolerance, Respectively, via Contrasting Stress-Amelioration Mechanisms

“…Pertinent to this property was a reported transcriptional profile of salt-stressed Arabidopsis roots that revealed the enrichment MATE transporters [ 97 ]. In addition, putative salt responsive MATE genes were identified in Arabidopsis, rice, and chickpea [ 98 ] whereas an Arabidopsis Golgi-localized MATE transporter was found to be induced by excessive iron and osmotic stress conditions [ 99 ]. In like manner, the up-regulation of a cyclic nucleotide gated Ca 2+ -permeable cation channel 5 gene in SS plants coincided with the proposed role for a similar channel in the conferral of salt tolerance to Arabidopsis [ 100 ].…”

Genome-Wide Analysis of Multidrug and Toxic Compound Extrusion (MATE) Family inGossypium raimondiiandGossypium arboreumand Its Expression Analysis Under Salt, Cadmium, and Drought Stress

“…In higher plants, the land plants that have lignified tissues (the xylem) for conducting water and minerals throughout the plant, the MATE genes have been found to be involved in the transportation and transiting of xenobiotic and other small organic molecules, such as inositol hexakisphosphate, yokonolide B, p -chlorophenoxyisobutyric acid, toyocamycin and terfestatin ( Diener et al 2001 ; Tiwari et al 2014 ). Salt-responsive genes belonging to MATE efflux proteins have been reported to play a significant role in conferring salt tolerance in rice and chickpea ( Nimmy et al 2015 ). In addition, putative salt-responsive genes from the model plant Arabidopsis thaliana encoding the MATE efflux family have been identified and found to enhance salt tolerance ( Li et al 2002 ).…”