Expression analysis of BsAPase14 acid phosphatase, a stress responsive boiling-stable protein from Triticum aestivum

Sharma, Arun Dev; Rakhra, Gurmeen; Singh, Jatinder

doi:10.1007/s12892-011-0054-y

Cited by 5 publications

(4 citation statements)

References 19 publications

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“…The boiling stable proteins were extracted as described previously [Sharma et al, 2006;Sharma et al, 2012]. Briefly, tissues were homogenized with chilled mortar and pestle in extraction buffer [50mM Tris buffer (pH 7.0)].…”

Section: Extraction Of Boiling Stable Proteinsmentioning

confidence: 99%

Accumulation of class-III type of boiling stable Peroxidases in response to plant growth hormone ABA in Triticum aestivum cultivars

Sharma¹,

Rakhra²,

Mamik³

et al. 2014

Plant Sci Today

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Abscisic acid (ABA) is a key plant growth and stress hormone involved in many biological processes. It has been shown to be involved in Reactive Oxygen Species (ROS) generation. Class-III Peroxidases (PODs) are known to maintain oxidative stress induced-ROS at sublethal levels in plants under abiotic stress conditions, but, studies documenting how ABA regulates boiling stable class-III PODs are still a matter of conjuncture. In this study, the ABA-induced changes on ROS and ROS scavenging class III boiling stable POD were studied in the embryos of different cultivars of wheat. Simultaneous analysis of ROS contents, activities of ROS-scavenging class-III boiling stable POD enzymes gave an integrative view of physiological state and detoxifying potential under conditions of sensitivity and tolerance. Indices of oxidative stress viz., superoxide radical and H2O2 content increased under ABA treatment in a genotype dependent manner. It was observed that cultivars: PBW 550, HD 2967 and PBW 621 have more efficient mechanism to scavenge ROS species as shown by increase in BsPOD activity accompanied by enhanced expression of boiling stable POD isoenzymes. Based on results it can be inferred that embryos of cvs. PBW 550, HD 2967 and PBW 621 have more capacity to perform biological antioxidative reactions to combat ABA-induced oxidative stress.

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Section: Extraction Of Boiling Stable Proteinsmentioning

confidence: 99%

Accumulation of class-III type of boiling stable Peroxidases in response to plant growth hormone ABA in Triticum aestivum cultivars

Sharma¹,

Rakhra²,

Mamik³

et al. 2014

Plant Sci Today

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“…The boiling soluble proteins were extracted as described previously (Sharma et al, 2012). The total soluble protein content was determined using BSA as a standard according to Lowry et al (1951).…”

Section: Extraction Of Boiling Soluble Proteinsmentioning

confidence: 99%

“…Hence, in order to find out the role of BSPs in drought stress adaptation, it is imperative that expression profile of BSPs should be studied in stress susceptible and tolerant cultivars of a crop. In the continuation of our previous study (Sharma et al, 2012), the present study was conducted in two different cultivars of wheat: 'PBW 527' (tolerant), 'PBW 343' (susceptible) so as to characterize their response to drought at protein level at different stages of their development. The assessment and expression analysis of BSPs observed in this study under drought stress could unravel the underlying mechanisms of drought tolerance and possible implication of BSPs as a biochemical marker for water stress adaptation.…”

Section: Introductionmentioning

confidence: 99%

Studies on the Accumulation of Drought-Induced Boiling Soluble Proteins (Hydrophilins) at Vegetative and Reproductive Phases of Drought Tolerant and Susceptible Cultivars of <i>Triticum aestivum</i>

2014

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Drought is one of the major environmental constraints affecting the crops worldwide. Expression of boiling soluble proteins (BSPs) is of paramount importance, because they play important roles in the water stress responses and also in plant metabolism.In this study, the effect of drought on BSPs at vegetative (shoots) and reproductive (seeds) phases of drought tolerant (cv. 'PBW 527') and drought susceptible (cv. 'PBW 343') cultivars of Triticum aestivum were carried at three different developmental stages.The boiling soluble protein profiles of shoots and seeds were outlined via SDS-PAGE followed by immune-blot analysis using anti-HSP, anti-APase, anti-LEA, anti-SOD, anti-AQUA and anti-CAT antibodies. Western blot analysis revealed that expression of BSPs was modulated differentially in a stress, tissue, developmental stage and cultivar dependent manner. For instance, enhanced expression of seeds BSPs (APase, LEA, CAT, AQUA) was observed in the tolerant cv. 'PBW 527' after drought stress.However, no such enhancement was observed in the susceptible cultivar. Similarly, in shoots of cv. 'PBW 527', a substantial increase of BSP (SOD) expression was established after drought stress treatment, indicating their role in drought stress adaptation.Further, to gain an insight into the role of BSPs, a time course pre-and post-stress kinetic studies were also conducted in the seeds of tolerant and susceptible wheat cultivars. Based upon the observations, the possible role of boiling soluble proteins (hydrophilins) in water stress tolerance is discussed.

show abstract

Section: Extraction Of Boiling Soluble Proteinsmentioning

confidence: 99%

Studies on the Accumulation of Drought-Induced Boiling Soluble Proteins (Hydrophilins) at Vegetative and Reproductive Phases of Drought Tolerant and Susceptible Cultivars of <i>Triticum aestivum</i>

2014

Self Cite

View full text Add to dashboard Cite

Drought is one of the major environmental constraints affecting the crops worldwide. Expression of boiling soluble proteins (BSPs) is of paramount importance, because they play important roles in the water stress responses and also in plant metabolism. In this study, the effect of drought on BSPs at vegetative (shoots) and reproductive (seeds) phases of drought tolerant (cv. ‘PBW 527’) and drought susceptible (cv. ‘PBW 343’) cultivars of Triticumaestivum were carried at three different developmental stages. The boiling soluble protein profiles of shoots and seeds were outlined via SDS-PAGE followed by immune-blot analysis using anti-HSP, anti-APase, anti-LEA, anti-SOD, anti-AQUA and anti-CAT antibodies. Western blot analysis revealed that expression of BSPs was modulated differentially in a stress, tissue, developmental stage and cultivar dependent manner. For instance, enhanced expression of seeds BSPs (APase, LEA, CAT, AQUA) was observed in the tolerant cv. ‘PBW 527’ after drought stress. However, no such enhancement was observed in the susceptible cultivar. Similarly, in shoots of cv. ‘PBW 527’, a substantial increase of BSP (SOD) expression was established after drought stress treatment, indicating their role in drought stress adaptation. Further, to gain an insight into the role of BSPs, a time course pre- and post-stress kinetic studies were also conducted in the seeds of tolerant and susceptible wheat cultivars. Based upon the observations, the possible role of boiling soluble proteins (hydrophilins) in water stress tolerance is discussed.

show abstract

Expression analysis of BsAPase14 acid phosphatase, a stress responsive boiling-stable protein from Triticum aestivum

Cited by 5 publications

References 19 publications

Accumulation of class-III type of boiling stable Peroxidases in response to plant growth hormone ABA in Triticum aestivum cultivars

Accumulation of class-III type of boiling stable Peroxidases in response to plant growth hormone ABA in Triticum aestivum cultivars

Studies on the Accumulation of Drought-Induced Boiling Soluble Proteins (Hydrophilins) at Vegetative and Reproductive Phases of Drought Tolerant and Susceptible Cultivars of <i>Triticum aestivum</i>

Studies on the Accumulation of Drought-Induced Boiling Soluble Proteins (Hydrophilins) at Vegetative and Reproductive Phases of Drought Tolerant and Susceptible Cultivars of <i>Triticum aestivum</i>

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