Expression analysis of self-incompatibility-associated genes in non-heading Chinese cabbage

Abstract

ABSTRACT. In Brassicaceae, a self-incompatibility (SI) system mediates pollen-pistil interactions. Self-pollen could be recognized and rejected by incompatible pistils. Several components involved in the SI response have been determined, including S-locus receptor kinase (SRK), S-locus cysteine-rich protein/S-locus protein 11, and arm repeatcontaining protein 1 (ARC1). However, the components involved in the SI system of Brassicaceae are not fully understood. Here, we detected expression patterns of 24 SI-related genes in non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino) after compatible and incompatible pollination, and potential interaction relationships of these genes were predicted. SRK and ARC1 transcripts increased initially 0.25 h after incompatible pollination, while kinase-associated protein phosphatase had an expression pattern that was opposite that of SRK transcripts during self-pollination. Plant U-box 8 was not required in the SI response of non-heading Chinese cabbage. Our results showed that the SI signal of non-heading Chinese cabbage could occur within 0.25 h after self-pollination. The hypothetical interaction relationships indicated that plastid-lipid-associated protein and malate dehydrogenase could be negatively regulated by chaperonin 10, glutathione transferase, cytidylate kinase/uridylate kinase, and methionine synthase by indirect interactions. Our findings could be helpful to better understand potential roles of these components in the SI system of non-heading Chinese cabbage.

Identification and Validation of Reference Genes for RT-qPCR Analysis in Non-Heading Chinese Cabbage Flowers

“…To validate the reference genes confirmed above, the relative expression profiles of SRK , which is expressed specifically in stigma (Stein et al, 1996 ) and Exo70A1 , which was reported to be without significant difference in expressions level between compatible and incompatible floral tissues (Wang et al, 2014b ) were measured and normalized with three sets of reference genes according to the rankings of reference genes mentioned above. Briefly, the most stable reference genes ( ACT7 and EF1 α for stigma samples of SI at different developmental stages, DNAJ and UKN1 for different floral tissues of SI samples and SC-SI mixed samples) either alone or combination, and the least stable reference gene ( ACP for stigma samples of SI at different developmental stages, CYP for different floral tissues of SI samples and SC-SI mixed samples), were used as reference genes for RT-qPCR analysis.…”

The poplar pangenome provides insights into the evolutionary history of the genus

“…The genes involved in reproduction encoded for S-locus lectin protein kinase family proteins, receptor protein kinases, receptor-like protein kinases, and lectin protein kinase family proteins (S-locus proteins hereafter), and about 38.21% of them were homologs of S-locus cysteine-rich protein (SCR) in Brassicaceae (Supplementary Data 11). The SCR gene encoded proteins as male determinants of recognition specificity and controlled self-incompatibility (SI) along with the S-locus receptor kinase (SRK) 31–33 . The large-effect SNPs and frameshift indels should cause the S-locus protein to lose its function and reduce the SI of the host.…”

Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum

“…Furthermore, some other proteins associated with oxidative phosphorylation, such as CAN70186 (spot 134), EMJ11768 (spot 139), also had higher expression levels at 2 h in the cross-pollination pistil. Research showed than NADPH was thought as SI related protein [ 47 ], so these proteins may act to maintain higher respiration rate to meet the demands for ATP in SI responses [ 48 ]. In addition, this kind of DEP exhibited in the early pollination response also indicated that the differential reaction referring to respiration demands for ATP may take place when pollen-stigma interacted.…”