Expression analysis of squalene synthase gene in mevalonate pathway of <i>Sanghuangporus baumii</i>

Wang, Shixin; Wang, Xutong; Liu, Zengcai; Sun, Tingting; Zou, Li

doi:10.1080/13102818.2022.2060759

Cited by 1 publication

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“…Some stress-responsive elements involved in the defense against environmental perturbations were also identified [34]. The most important thing to note is that all of these gene promoters contain the MeJA responsive element, the CGTCA motif, which is also an important reason why they are closely related to the accumulation of triterpenoids in response to MeJA treatment [25,26]. These results are basically consistent with previous conclusions, "MeJA acts on CGTCA-motif elements of key gene promoter through signal transduction.…”

Section: Discussionsupporting

confidence: 88%

“…To determine whether the expression of the five triterpenoid biosynthesis genes respond to MeJA treatment, we cloned and analyzed their promoter regions. Among them, the SQS and LS gene promoters have been shown to contain MeJA-responsive elements [25,26]. We also identified MeJA-responsive elements in the promoters of the other three genes (Figure 3a-c).…”

Section: Analysis Of the Promoter Regions Of Five Genes Involved In T...mentioning

confidence: 67%

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New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species Sanghuangporusbaumii (Pilát) L.W. Zhou & Y.C. Dai

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Tong

et al. 2022

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Triterpenoids are secondary metabolites produced by the fungus Sanghuangporus baumii that have important pharmacological activities. However, the yield of triterpenoids is low and cannot meet market demand. Here, we treated S. baumii with several concentrations of MeJA (methyl jasmonate) and found that the total triterpenoid content was highest (23.31 mg/g) when the MeJA concentration was 250 μmol/L. qRT-PCR was used to quantify the transcription of five key genes involved in triterpenoid biosynthesis. The results showed that the relative transcription of most genes increased with increasing MeJA concentration, indicating that MeJA is a potent inducer of triterpenoid biosynthesis in S. baumii. To further explore whether other terpenoid biosynthesis pathways are also involved in the accumulation of triterpenoids induced by MeJA, we measured the contents of cis-Zeatin (cZ), gibberellins (GAs), and the transcript levels of related biosynthesis genes. We found that MeJA significantly inhibited the biosynthesis of cZ, GAs, and the transcription of related genes. The repressive effects of MeJA on cZ and GA accumulation were further confirmed by growth rate and biomass assays. In conclusion, our study provides an effective method to enhance the triterpenoid content of S. baumii, and also provides novel insights into the mechanism of MeJA-induced triterpenoid biosynthesis.

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Section: Discussionsupporting

confidence: 88%

Section: Analysis Of the Promoter Regions Of Five Genes Involved In T...mentioning

confidence: 67%

New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species Sanghuangporusbaumii (Pilát) L.W. Zhou & Y.C. Dai

Liu

Tong

et al. 2022

JoF

Self Cite

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show abstract

Expression analysis of squalene synthase gene in mevalonate pathway of Sanghuangporus baumii

Cited by 1 publication

References 27 publications

New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species Sanghuangporusbaumii (Pilát) L.W. Zhou & Y.C. Dai

New Insights into Methyl Jasmonate Regulation of Triterpenoid Biosynthesis in Medicinal Fungal Species Sanghuangporusbaumii (Pilát) L.W. Zhou & Y.C. Dai

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