Expression Analysis of Two Cancer-testis Genes, FBXO39 and TDRD4, in Breast Cancer Tissues and Cell Lines

Abstract

Breast cancer accounts for one third of new cancer cases among women. The need for biomarkers for early detection is the stimulus to researchers to evaluate altered expression of genes in tumours. Cancer-testis (CT) genes are a group with limited expression in normal tissues except testis but up-regulation in a wide variety of cancers. We here evaluated expression of two CT genes named FBXO39 and TDRD4 in 32 invasive ductal carcinoma samples, 10 fibroadenomas and 6 normal breast tissue samples, in addition to two breast cancer cell lines, MCF-7 and MDA-MB-231, by the means of quantitative real time RT-PCR. FBXO39 showed significant up-regulation in invasive ductal carcinoma samples in comparison with normal samples. It also was expressed in both cell lines and after RHOXF1 gene knock down it was down-regulated in MCF-7 but up-regulated in the MDA-MB-231 cell line. TDRD4 was not expressed in the MCF-7 cell line and any of the tissue samples except testis. However, it was expressed in MDA-MB-231 and was up-regulated after RHOXF1 gene knock down. Our results show that FBXO39 but not TDRD4 can be used for cancer detection and if proved to be immunogenic, might be a putative candidate for breast cancer immunotherapy.

Lactobacillus acidophilus and Lactobacillus crispatus Culture Supernatants Downregulate Expression of Cancer-testis Genes in the MDA-MB-231 Cell Line

“…In addition, they have been shown to regulate gene expression by simultaneous decrease in histone acetylation and increase in DNA methylation (Ghadimi et al, 2012). Cancer-testis antigens (CTAs) are a group of tumor associated antigens with restricted expression in normal tissues except testis and expression in different tumors (Seifi-Alan et al, 2013). Their expression has been shown to be epigenetically regulated (Ghafouri-Fard and Modarressi, 2009).…”

Roles of the Bcl-2/Bax Ratio, Caspase-8 and 9 in Resistance of Breast Cancer Cells to Paclitaxel

“…It is susceptible to apoptosis and applied for various investigations on apoptosis or survival of cancer cells (Simstein et al, 2003;Ghafouri-Fard et al, 2012;Hamedeyazdan et al, 2012;Darakhshan et al, 2013;Seifi-Alan et al, 2013). Developing resistant cancer cells to specific chemotherapeutic agents can provide valuable model systems to investigate molecular mechanisms of chemoresistance (Rastogi and Sinha, 2009;McDermott et al, 2014).…”

Knockdown of FBXO39 inhibits proliferation and promotes apoptosis of human osteosarcoma U‑2OS cells

“…F-box proteins recognize and combine substrates in the SCF complex, and participate in a series of cellular processes, including cell cycle and immune responses (10)(11)(12)(13). It has been reported that F-box protein 39 (FBXO39) was highly expressed in normal human testis and abnormally expressed in a variety of cancer cell types (14,15). Furthermore, it has been demonstrated that patients with colon cancer exhibit a high level of serum FBXO39 expression compared with healthy individuals (14,15).…”