Expression and activity of trypsin and pepsin during larval development of Indian walking catfish ( Clarias magur )

Mir, Ishfaq Nazir; Srivastava, Prem Prakash; Bhat, Irfan Ahmad; Muralidhar, A. P.; Varghese, Tincy; Gireesh‐Babu, P.; Jain, Kartik

doi:10.1016/j.aquaculture.2018.03.049

Cited by 23 publications

(15 citation statements)

References 34 publications

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“…From then on, the expression of pep gene stained sharply increasing until 30 dph. This finding is in agreement with the early detection of pep gene expression in other fish species: Pagrus major [1,37] , Clarias magur [8] , Acipenser persicus [26] . In the present study, the pattern of specific pepsin activity was similar with the total activity, the pepsin activity was detected at hatching and then showed slight fluctuations until 15 dph.…”

Section: Discussionsupporting

confidence: 89%

“…The expression of try in S. chuatsi larvae were detected at hatching and stained slight fluctuations until 15 dph, these results agree with other reports in larvae of several fish species [1,11,26] . Then, the expression of try gradually decreased until 30 dph, similar findings about try expression have been reported in other species such as Lates calcarifer [3] , Clarias magur [8] , Lutjanus guttatus [27] and…”

Section: Discussionsupporting

confidence: 85%

“…As the same time, it has been considered that the zooplankton not necessarily possess the adequate nutrients for S. chuatsi, which can cause this species show strong piscivory in the early larval stage [8] . In this way, if we want to improve the survival during the larval stage, it is necessary to conduct specific studies, based on in digestive physiology of species, that allow for evaluation of the digestive enzymatic capacity of the larvae.…”

Section: Introductionmentioning

confidence: 99%

“…It is necessary to evaluate the comprehensively digestive ability of S. chuatsi larva from the aspects of gene expression and enzyme activity detection. Recently, the use of molecular biological approaches have complemented traditional methods in expressing the profiles of digestive enzyme precursors in fish [1,8,11,12] and thus providing insight into both temporal and spatial expression patterns of genes involved in the development and functionality of digestive systems during early ontogeny. As such, this study looks to investigate the early ontogeny of S. chuatsi through an integrative investigation to understand the general patterns of activity through the effect of feeding status, morphological changes, and gene expression on final enzymatic capacity.…”

Section: Introductionmentioning

confidence: 99%

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Development of digestive enzymatic activity and gene expression during the early ontogeny of Chinese perch (Siniperca chuatsi)

Tang

Liang

et al. 2021

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Chinese perch (Siniperca chuatsi) is one of the economically important freshwater species fish for aquaculture in China. This study aimed to determine the ontogenetic development of the digestive enzyme activity (trypsin, pepsin, amylase, lipase, chymotrypsin and alkaline phosphatase) and related gene expressions of S. chuatsi larvae from hatching to 30 days post-hatching (dph). The larvae were fed with live fry fish twice a day. Results indicated that it was low detection of enzyme activity and gene expression of trypsin, chymotrypsin, lipase, amylase and alkaline phosphatase before mouth opened, the last two enzymes showed an activity close to zero. Different from other carnivorous fish, specific activity and gene expression of trypsin, chymotrypsin and lipase in S. chuatsi larvae were not increased after starting the first feeding. Interestingly, the amylase and alkaline phosphatase specific activity progressively increased over development, indicating that the larvae have certain ability to digest carbohydrates. Pepsin activity and gene expression started to sharply increase after 15 dph, other digestive enzyme activity showed downward trends. The development pattern of digestive enzymes may affect the ability of S. chuatsi to digest the zooplankton, which leads to the formation of unique feeding habit of the S. chuatsi larvae. This study also will provide the necessary theoretical basis for the artificial opening diet of the S. chuatsi larvae.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of digestive enzymatic activity and gene expression during the early ontogeny of Chinese perch (Siniperca chuatsi)

Tang

Liang

et al. 2021

Preprint

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“…Total RNA was extracted from the whole larval samples taken at the abovementioned time points of the study, using Trizol TM reagent (Invitrogen, United States) as per manufacturer's instructions. The genomic DNA was removed from the extracted RNA using DNase I (Thermo Scientific, United States) treatment following our previously published study (Mir et al, 2018b). The concentration of RNA as well as the purity was determined by NanoDrop spectrophotometer (Thermo Scientific, United States) at an optical density of 260/280.…”

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels

et al. 2020

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This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae.

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Expression and activity of key lipases during the larval development of walking catfish (Clarias magur)

et al. 2019

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The study was conducted to investigate the expression and activity of key lipolytic enzymes during the ontogenetic development of Clarias magur. After partial characterization, the messenger RNA (mRNA) expression analysis of lipoprotein lipase (LPL), pancreatic triacylglycerol lipase (PL), and bile salt-activated lipase (BAL) genes along with the specific lipase activity were performed in larvae from Day 1 after hatching till 34-day posthatch (dph). Heterogeneous patterns of mRNA expression were shown by the important lipolytic enzymes and were detected before first exogenous feeding during the yolk-sac stage. LPL started increasing from 13 dph and peaked at 16 dph followed by a declining trend till 34 dph. However, the PL observed to be peaking at 9, 22, and 30 dph. Similarly, BAL showed an increasing trend from 11 to 22 dph with a significantly high level of mRNA expression at 16 dph. Later, the specific lipase activity was evaluated which appears at Day 1 after hatching with a progressive increase from 7 to 16 dph and a further declining trend afterwards with a peak at 22 dph. The results indicated the development of exocrine pancreas at 16 dph. Furthermore, the transcript levels and the activity of lipases were regulated with the age. Hence, the present study can be helpful in devising different strategies containing optimum lipid levels at a suitable stage of development for improving the survival during larval rearing. Furthermore, the study could be a baseline for elucidating the optimized dietary lipid levels of this catfish during its larval rearing.

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Expression and activity of trypsin and pepsin during larval development of Indian walking catfish ( Clarias magur )

Cited by 23 publications

References 34 publications

Development of digestive enzymatic activity and gene expression during the early ontogeny of Chinese perch (Siniperca chuatsi)

Development of digestive enzymatic activity and gene expression during the early ontogeny of Chinese perch (Siniperca chuatsi)

Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels

Expression and activity of key lipases during the larval development of walking catfish (Clarias magur)

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