Expression and activity of V‐H⁺‐ATPase in gill and kidney of marbled eel Anguilla marmorata in response to salinity challenge

Abstract

The full-length complementary (c)DNA of vacuolar-type-H(+) -ATPase B1 gene (vhab1) in marbled eel Anguilla marmorata with 1741 base pairs (bp) was identified. It contained a 1512 bp open reading frame encoding a polypeptide with 503 amino acids (55·9 kDa), an 83 bp 5'-untranslated region (UTR) and a 146 bp 3'-UTR. The expression levels of A. marmorata vhab1 in gill and kidney of A. marmorata were evaluated at different intervals during the exposure to various salinities (0, 10 and 25). The results indicated that the expression levels of A. marmorata vhab1 messenger (m)RNA in gill and kidney had a significant increase and reached the highest level at 1 h in brackish water (BW, salinity 10) group and 6 h in seawater (SW, salinity 25) group. Therefore, salinity did affect the relative expression level of A. marmorata vhab1 mRNA in gills, which exhibited the enhancement by c. 44 times in SW group when compared with that in fresh water. No remarkable difference in the expression of A. marmorata vhab1 mRNA was observed after 15 days of SW exposure (P > 0·05). V-H(+) -ATPase activity exhibited an increase by two- to three-fold when compared with that in gill and kidney from the control group. The consequence primarily suggested that A. marmorata vhab1 gene product in elvers from A. marmorata plays an important role in adaptation response to SW.

V-ATPase subunit B plays essential roles in the molting process of Chinese mitten crab, Eriocheir sinensis

“…The tissue expression pattern of EsVATB is similar to that of other aquatic crustaceans, with the highest expression identified in the osmoregulatory organ, that is, the gills (Ali et al, 2017;Li et al, 2015). Crustacean gills are a multifunctional organ essential for osmotic and ionic homeostasis, except for their role in gas exchange in crustaceans (Freire et al, 2008).…”

Draft genome assembly of Tenualosa ilisha, Hilsa shad, provides resource for osmoregulation studies

“…The kidney is an important organ in euryhaline fish, for ion homeostasis and osmoregulation of body fluids during the process of adaptation to various salinities 66 . In Hilsa, Under water homeostasis category, highest number of genes were found in renal water homeostasis, which may be the indication of importance of this process in Hilsa.…”

Physiological mechanism of osmoregulatory adaptation in anguillid eels

“…In FW teleosts, the nephron consists of a renal corpuscle and proximal and distal renal tubules, followed by a collecting duct, whereas nephrons in SW teleosts typically lack the distal tubule (Akira et al 2005 ; Teranishi 2010 ). In particular, the kidney plays a major role in euryhaline teleost such as eel as it helps in maintaining body fluid homeostasis during the course of adaptation to different salinities (Li et al 2015 ; Martinez et al 2005a ; Teranishi et al 2013 ). The kidney is fully by glomerular functions as in any other euryhaline fish (Cliff and Beyenbach 1988 ; Kenyon et al 1985 ; Teranishi 2010 ).…”

“…Guanylin, uroguanylin, and lympho-guanylin are members of a family of heat-stable peptides that mediate their actions via the guanylate cyclase receptor isoform termed GC-C (Cutler et al 2007 ; Li et al 2015 ), which is likely to play a major role in osmoregulation in both freshwater and marine teleosts (Comrie et al 2001 ). The major function of guanylin is regulation of water and electrolytes (particularly Cl − ) transport of the intestine, and uroguanylin also participates in renal handling of water and electrolytes (Yuge et al 2003 ).…”